Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells.

Abstract:

:Visualization of morphological dynamics of live cells with nanometer resolution under physiological conditions is highly desired, but challenging. It has been demonstrated that high-speed atomic force microscopy is a powerful technique for visualizing dynamics of biomolecules under physiological conditions. However, application of high-speed atomic force microscopy for imaging larger objects such as live mammalian cells has been complicated because of the collision between the cantilever and samples. Here, we demonstrate that attaching an extremely long (~3 μm) and thin (~5 nm) tip by amorphous carbon to the cantilever allows us to image the surface structure of live cells with the spatiotemporal resolution of nanometers and seconds. We demonstrate that long-tip high-speed atomic force microscopy is capable of imaging morphogenesis of filopodia, membrane ruffles, pit formation, and endocytosis in COS-7, HeLa cells and hippocampal neurons.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Shibata M,Uchihashi T,Ando T,Yasuda R

doi

10.1038/srep08724

subject

Has Abstract

pub_date

2015-03-04 00:00:00

pages

8724

issn

2045-2322

pii

srep08724

journal_volume

5

pub_type

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