CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through modulation of TGF-β signaling.

Abstract:

:Previous studies have shown that CD61 (integrin-β3) promotes the differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) into germ-like cells. However, the mechanism remains unclear. In this study, we showed that overexpression of CD61 in canine adipose-derived mesenchymal stem cells (cADMSCs) promotes their differentiation into primordial germ cell (PGC)-like cells. Quantitative real-time PCR, immunocytochemistry and western blot detected higher levels of PGC-specific markers in CD61-overexpressed cADMSCs compared with those in control cells. Moreover, phosphorylation of Smad2, a downstream mediator of transforming growth factor beta (TGF-β), was increased in CD61-overexpressed cADMSCs than that in control cells. However, the expression of PGC-specific markers was downregulated in cADMSCs treated with a TGF-β inhibitor. These results suggested that CD61 could induce cADMSCs to differentiate into PGC-like cells by relying on the activation of TGF-β pathway. ADMSCs possess a considerable potential in treating the infertility of rare animal species.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Fang J,Wei Y,Lv C,Peng S,Zhao S,Hua J

doi

10.1038/srep43851

subject

Has Abstract

pub_date

2017-03-03 00:00:00

pages

43851

issn

2045-2322

pii

srep43851

journal_volume

7

pub_type

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