Abstract:
:Proton-sensing ovarian cancer G-protein coupled receptor (OGR1) plays an important role in pH homeostasis. Acidosis occurs at sites of intestinal inflammation and can induce endoplasmic reticulum (ER) stress and the unfolded protein response (UPR), an evolutionary mechanism that enables cells to cope with stressful conditions. ER stress activates autophagy, and both play important roles in gut homeostasis and contribute to the pathogenesis of inflammatory bowel disease (IBD). Using a human intestinal epithelial cell model, we investigated whether our previously observed protective effects of OGR1 deficiency in experimental colitis are associated with a differential regulation of ER stress, the UPR and autophagy. Caco-2 cells stably overexpressing OGR1 were subjected to an acidic pH shift. pH-dependent OGR1-mediated signalling led to a significant upregulation in the ER stress markers, binding immunoglobulin protein (BiP) and phospho-inositol required 1α (IRE1α), which was reversed by a novel OGR1 inhibitor and a c-Jun N-terminal kinase (JNK) inhibitor. Proton-activated OGR1-mediated signalling failed to induce apoptosis, but triggered accumulation of total microtubule-associated protein 1 A/1B-light chain 3, suggesting blockage of late stage autophagy. Our results show novel functions for OGR1 in the regulation of ER stress through the IRE1α-JNK signalling pathway, as well as blockage of autophagosomal degradation. OGR1 inhibition might represent a novel therapeutic approach in IBD.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Maeyashiki C,Melhem H,Hering L,Baebler K,Cosin-Roger J,Schefer F,Weder B,Hausmann M,Scharl M,Rogler G,de Vallière C,Ruiz PAdoi
10.1038/s41598-020-57657-9subject
Has Abstractpub_date
2020-01-29 00:00:00pages
1438issue
1issn
2045-2322pii
10.1038/s41598-020-57657-9journal_volume
10pub_type
杂志文章abstract::We report localised-surface-plasmon (LSP) enhanced deep-ultraviolet light-emitting diodes (deep-UV LEDs) using Al nanoparticles for LSP coupling. Polygonal Al nanoparticles were fabricated on the top surfaces of the deep-UV LEDs using the oblique-angle deposition method. Both the top- and bottom-emission electrolumine...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04380
更新日期:2014-03-14 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-62685-6
更新日期:2020-04-02 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) is associated with cognitive impairment. We investigated whether alterations of intranetwork and internetwork functional connectivity with T2DM progression exist, by using resting-state functional MRI. MRI data were analysed from 19 T2DM patients with normal cognition (DMCN) and 19 T2DM...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32980
更新日期:2016-09-13 00:00:00
abstract::Recently, there has been a debate whether implicit Theory of Mind can be reliably measured using anticipatory looking tasks. Previous anticipatory looking paradigms used video stimuli to measure implicit Theory of Mind; however, numerous replications of these paradigms were unsuccessful. This lack of replications may ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80614-5
更新日期:2021-01-13 00:00:00
abstract::Histological chorioamnionitis (HCA) is an infection of fetal membranes and complicates 5.2% to 28.5% of all live births. HCA is associated with increased mortality and morbidity in both premature and term neonates. Exposure to HCA may have long-term consequences, including an increased risk for allergic disorders and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42205-x
更新日期:2019-04-10 00:00:00
abstract::Aging is a highly controlled biological process characterized by a progressive deterioration of various cellular activities. One of several hallmarks of aging describes a link to transcriptional alteration, suggesting that it may impact the steady-state mRNA levels. We analyzed the mRNA steady-state levels of polyCAG-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10692
更新日期:2015-06-02 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-67918-2
更新日期:2020-07-09 00:00:00
abstract::Elevated ground-level ozone (O3), which is an important aspect of air quality related to public health, has been causing increasing concern. This study investigated the spatiotemporal distribution of ground-level O3 concentrations in China using a dataset from the Chinese national air quality monitoring network during...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03929-w
更新日期:2017-06-16 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-23445-9
更新日期:2018-03-22 00:00:00
abstract::Drug-induced cholestasis is a leading cause of drug withdrawal. However, the use of primary human hepatocytes (PHHs), the gold standard for predicting cholestasis in vitro, is limited by their high cost and batch-to-batch variability. Mature hepatocyte characteristics have been observed in human induced hepatocytes (h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38694
更新日期:2016-12-09 00:00:00
abstract::Brain imaging genetics intends to uncover associations between genetic markers and neuroimaging quantitative traits. Sparse canonical correlation analysis (SCCA) can discover bi-multivariate associations and select relevant features, and is becoming popular in imaging genetic studies. The L1-norm function is not only ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13930-y
更新日期:2017-10-25 00:00:00
abstract::This prospective multicenter randomized comparative cross-over trial aimed at evaluating the influence of hemodialysis vs post-dilution hemodiafiltration with high-flux dialyzers in solute clearance and biocompatibility profile. 32 patients were sequentially dialyzed with Leoceed-21HX, Polypure-22S+, Rexsys-27H and VI...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/s41598-019-54404-7
更新日期:2019-12-04 00:00:00
abstract::Polar bears are ice-associated marine mammals that are known to swim and dive, yet their aquatic behaviour is poorly documented. Reductions in Arctic sea ice are clearly a major threat to this species, but understanding polar bears' potential behavioural plasticity with respect to the ongoing changes requires knowledg...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27947-4
更新日期:2018-06-26 00:00:00
abstract::Production of reactive nitrogen species (NO) is a key step in the immune response following infections. NO induces lesions to bacterial DNA, thus limiting bacterial growth within hosts. Using two pathogenic bacteria, Bacillus cereus and Shigella flexneri, we show that the DNA-repair protein Mfd (Mutation-Frequency-Dec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29349
更新日期:2016-07-20 00:00:00
abstract::Cardiovascular diseases are directly linked to smoking habits, which has both physiological and anatomical effects on the systemic and retinal circulations, and these changes can be detected with fundus photographs. Here, we aimed to 1- design a Convolutional Neural Network (CNN), using retinal photographs, to differe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43670-0
更新日期:2019-05-09 00:00:00
abstract::Plant genomes encode several classes of small regulatory RNAs (sRNAs) that play critical roles in both development and stress responses. Panax notoginseng (Burk.) F.H. Chen (P. notoginseng) is an important traditional Chinese herbal medicinal plant species for its haemostatic effects. Therefore, the root yield of P. n...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09670-8
更新日期:2017-08-25 00:00:00
abstract::To tailor the properties of colloidal materials, precise control over the self-assembly of their constituents is a prerequisite. Here, we govern the assembly of silica particles by functionalization with supramolecular moieties which interact with each other via directional and reversible hydrogen bonding. Through a g...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19282-5
更新日期:2018-01-19 00:00:00
abstract::This research aimed to explore the potential and mechanism of heat modified sawdust combined with Festuca arundinacea for the remediation of Pb-contaminated soil. We determined Pb concentration and biochemical indices in plants and soils, analyzed microbial communities in soil, and studied Pb distribution in subcellul...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61668-x
更新日期:2020-03-13 00:00:00
abstract::Models of exchange-bias in thin films have been able to describe various aspects of this technologically relevant effect. Through appropriate choices of free parameters the modelled hysteresis loops adequately match experiment, and typical domain structures can be simulated. However, the use of these parameters, notab...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04508
更新日期:2014-03-28 00:00:00
abstract::Studies in mice suggest that early life represents a critical time window, where antibiotics may exert profound and lasting effects on the gut microbiota and metabolism. We aimed to test the hypothesis that prenatal antibiotic exposure is associated with increased risk of childhood overweight in a population-based coh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48065-9
更新日期:2019-08-08 00:00:00
abstract::Quantum physics is undoubtedly the most successful theory of the microscopic world, yet the complexities which arise in applying it even to simple atomic and molecular systems render the description of basic collision probabilities a formidable task. For this reason, approximations are often employed, the validity of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33425-8
更新日期:2018-10-10 00:00:00
abstract::Bacterial environmental and surgical site infection (SSI) surveillance was implemented from 2011-2016 in a UK Equine Referral Veterinary Hospital and identified 81 methicillin-resistant Staphylococcus aureus (MRSA) isolates. A cluster of MRSA SSIs occurred in early 2016 with the isolates confirmed as ST398 by multiloc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05559-8
更新日期:2017-07-14 00:00:00
abstract::Soils - constituting the largest terrestrial carbon pool - are vulnerable to climatic warming. Currently existing uncertainties regarding carbon fluxes within terrestrial systems can be addressed by studies of past carbon cycle dynamics and related climate change recorded in sedimentary successions. Here we show an ex...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31930
更新日期:2016-08-24 00:00:00
abstract::Zolpidem is not a typical GABAA receptor hypnotic. Unlike benzodiazepines, zolpidem modulates tonic GABA currents in the rat dorsal motor nucleus of the vagus, exhibits residual effects in mice lacking the benzodiazepine binding site, and improves speech, cognitive and motor function in human patients with severe brai...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28674
更新日期:2016-06-27 00:00:00
abstract::Cellular reprogramming involves profound alterations in genome-wide gene expression that is precisely controlled by a hypothetical epigenetic code. Small molecules have been shown to artificially induce epigenetic modifications in a sequence independent manner. Recently, we showed that specific DNA binding hairpin pyr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00544
更新日期:2012-01-01 00:00:00
abstract::Anticarsia gemmatalis (velvetbean caterpillar) and Chrysodeixis includens (soybean looper) are two important defoliation pests of soybeans. In the present study, we have investigated the susceptibility and brush border membrane-binding properties of both species to Bacillus thuringiensis Cry1Ea toxin. Bioassays perfor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54850-3
更新日期:2019-12-03 00:00:00
abstract::A new species of fossil conifer wood, Podocarpoxylon donghuaiense sp. nov., is described from the late Eocene of Nadu Formation in Baise Basin of the Guangxi Province, South China. This fossil wood is characterized by distinct growth rings, circular to oval tracheids in cross section, 1-2-seriate opposite pits on radi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32294
更新日期:2016-08-30 00:00:00
abstract::We performed continuous recordings (May 2015 - January 2017) of hydraulic pressure and electrical conductivity of groundwater in the 190 m-long horizontal S13 borehole drilled next to the deep underground laboratories of Gran Sasso (LNGS-INFN), located in the core of the Gran Sasso carbonate aquifer (central Italy) at...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34444-1
更新日期:2018-10-29 00:00:00
abstract::Curcumin is an important bioactive component of turmeric and also one of the important natural products, which has been investigated extensively. The precise mode of action of curcumin and its impact on system level protein networks are still not well studied. To identify the curcumin governed regulatory action on pro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69011-0
更新日期:2020-07-21 00:00:00
abstract::Sweet potato is an important food and bio-energy crop, and investigating the mechanisms underlying salt tolerance will provide information for salt-tolerant breeding of this crop. Here, the root transcriptomes of the salt-sensitive variety Lizixiang and the salt-tolerant line ND98 were compared to identify the genes a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40819
更新日期:2017-01-13 00:00:00