Abstract：
:Filamentous fungi naturally grow on solid surfaces, yet most genetic and biochemical analyses are still performed in liquid cultures. Here, we report a multiplexing platform using high-throughput photometric continuous reading that allows parallel quantification of hyphal growth and reporter gene expression directly on solid medium, thereby mimicking natural environmental conditions. Using this system, we have quantified fungal growth and expression of secondary metabolite GFP-based reporter genes in saprophytic Aspergillus and phytopathogenic Fusarium species in response to different nutrients, stress conditions and epigenetic modifiers. With this method, we provide not only novel insights into the characteristic of fungal growth but also into the metabolic and time-dependent regulation of secondary metabolite gene expression.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Cánovas D,Studt L,Marcos AT,Strauss Jdoi
10.1038/s41598-017-03598-9subject
Has Abstractpub_date
2017-06-27 00:00:00pages
4289issue
1issn
2045-2322pii
10.1038/s41598-017-03598-9journal_volume
7pub_type
杂志文章abstract：:H5N1 highly pathogenic avian influenza virus (HPAIV) poses a huge threat to public health and the global economy. These viruses cause systemic infection in poultry and accidental human infection leads to severe pneumonia, associated with high mortality rates. The hemagglutinin (HA) of H5N1 HPAIV possesses multiple bas...
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pub_type： 杂志文章
doi：10.1038/s41598-020-74604-w
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journal_title：Scientific reports
pub_type： 杂志文章,已发布勘误
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abstract：:Somatic mutations of many cancer genes tend to co-occur (termed co-mutations) in certain patterns during tumor initiation and progression. However, the genetic and epigenetic mechanisms that contribute to the co-mutations of these cancer genes have yet to be explored. Here, we systematically investigated the associati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35270
更新日期：2016-10-20 00:00:00
abstract：:Huntington's disease (HD) is a monogenetic neurodegenerative disorder that is caused by the expansion of a polyglutamine region within the huntingtin (HTT) protein, but there is still an incomplete understanding of the molecular mechanisms that drive pathology. Expression of the mutant form of HTT is a key aspect of d...
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pub_type： 杂志文章
doi：10.1038/s41598-020-78790-5
更新日期：2020-12-17 00:00:00
abstract：:Endothelial and mesenchymal stromal cells (ECs/MSCs) are crucial components of hematopoietic bone marrow stem cell niches. Both cell types appear to be required to support the maintenance and expansion of multipotent hematopoietic cells, i.e. hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs). With the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49221-x
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abstract：:Invasive species are characterized by their ability to colonize new habitats and establish populations away from their native range. In this sense, these plants are expected to have plastic responses to adapt to the environmental pressures during the invasion process. Hence, the role of natural selection is essential ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76922-5
更新日期：2020-11-18 00:00:00
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pub_type： 杂志文章
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abstract：:Clinical data from 172 cases of uterine fibroids with different appearances on MRI-T2WI and accepted ultrasound guided high intensity focused ultrasound (USgHIFU) treatment were retrospectively analyzed. This study aimed to evaluate the clinical safety and efficacy of ablating different types of fibroids, classified b...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-09-07 00:00:00
abstract：:Rising global temperatures are proving to be detrimental for the agriculture. Hence, strategies are needed to induce thermotolerance in food crops to sustain the food production. GABA (γ-aminobutyric acid), a non-protein amino acid, can partially protect plants from high-temperature stress. This study hypothesises tha...
journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32043
更新日期：2016-08-24 00:00:00
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pub_type： 临床试验,杂志文章
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更新日期：2017-06-16 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-11-25 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43832
更新日期：2017-03-07 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25904-9
更新日期：2018-05-22 00:00:00
abstract：:The Genomics of Drug Sensitivity in Cancer (GDSC) and Cancer Cell Line Encyclopedia (CCLE) are two major studies that can be used to mine for therapeutic biomarkers for cancers of a large variety. Model validation using the two datasets however has proved challenging. Both predictions and signatures do not consistentl...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-11-09 00:00:00
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journal_title：Scientific reports
pub_type： 临床试验,杂志文章
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更新日期：2019-01-24 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-12-11 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-09-27 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-08-30 00:00:00
abstract：:Intravitreal Conbercept (IVC) is the latest applied and effective treatment for the management of retinopathy of prematurity (ROP). However, conbercept escapes from the vitreous into the general circulation and reduce systemic VEGF concentrations. Thus, there are concerns about systemic complications, in these prematu...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-07-16 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17891-0
更新日期：2017-12-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22055-9
更新日期：2018-02-28 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-05-03 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16414-1
更新日期：2017-11-23 00:00:00
abstract：:Previous work has shown that human vision performs spatial integration of luminance contrast energy, where signals are squared and summed (with internal noise) over area at detection threshold. We tested that model here in an experiment using arrays of micro-pattern textures that varied in overall stimulus area and sp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29764
更新日期：2016-07-27 00:00:00
abstract：:Electrophysiological recordings in cortex in vivo have revealed a rich variety of dynamical regimes ranging from irregular asynchronous states to a diversity of synchronized states, depending on species, anesthesia, and external stimulation. The average population firing rate in these states is typically low. We study...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-09-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69382-4
更新日期：2020-07-23 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54956-8
更新日期：2019-12-09 00:00:00
