Abstract:
:In this study we characterise three tandem promoters (PR1-1, PR1-2 and PR1-3) within the PR1 regulatory region of the Escherichia coli kps capsule gene cluster. Transcription from promoter PR1-2 was dependent on the activity of the upstream promoter PR1-1, which activated PR1-2 via transcription coupled DNA supercoiling. During growth at 37 °C a temporal pattern of transcription from all three promoters was observed with maximum transcriptional activity evident during mid-exponential phase followed by a sharp decrease in activity as the cells enter stationary phase. The growth phase dependent transcription was regulated by Integration Host Factor (IHF), which bound within the PR1 region to repress transcription from PR1-2 and PR1-3. This pattern of transcription was mirrored by growth phase dependent expression of the K1 capsule. Overall these data reveal a complex pattern of transcriptional regulation for an important virulence factor with IHF playing a role in regulating growth phase expression.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Jia J,King JE,Goldrick MC,Aldawood E,Roberts ISdoi
10.1038/s41598-017-17891-0subject
Has Abstractpub_date
2017-12-20 00:00:00pages
17924issue
1issn
2045-2322pii
10.1038/s41598-017-17891-0journal_volume
7pub_type
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pub_type: 临床试验,杂志文章
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