Abstract：
:Helicobacter pullorum is an avian bacterium that causes gastroenteritis, intestinal bowel and hepatobiliary diseases in humans. Although H. pullorum has been shown to activate the mammalian innate immunity with release of nitric oxide (NO), the proteins that afford protection against NO and reactive nitrogen species (RNS) remain unknown. Here several protein candidates of H. pullorum, namely a truncated (TrHb) and a single domain haemoglobin (SdHb), and three peroxiredoxin-like proteins (Prx1, Prx2 and Prx3) were investigated. We report that the two haemoglobin genes are induced by RNS, and that SdHb confers resistance to nitrosative stress both in vitro and in macrophages. For peroxiredoxins, the prx2 and prx3 expression is enhanced by peroxynitrite and hydrogen peroxide, respectively. Mutation of prx1 does not alter the resistance to these stresses, while the single ∆prx2 and double ∆prx1∆prx2 mutants have decreased viability. To corroborate the physiological data, the biochemical analysis of the five recombinant enzymes was done, namely by stopped-flow spectrophotometry. It is shown that H. pullorum SdHb reacts with NO much more quickly than TrHb, and that the three Prxs react promptly with peroxynitrite, Prx3 displaying the highest reactivity. Altogether, the results unveil SdHb and Prx3 as major protective systems of H. pullorum against nitrosative stress.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Parente MR,Forte E,Falabella M,Boneca IG,Teixeira M,Giuffrè A,Saraiva LMdoi
10.1038/s41598-017-10375-1subject
Has Abstractpub_date
2017-08-30 00:00:00pages
9909issue
1issn
2045-2322pii
10.1038/s41598-017-10375-1journal_volume
7pub_type
杂志文章abstract：:The therapeutic management and health challenges caused by atrial fibrillation (AF) differ between different groups. The purpose of this study was to investigate the clinical features of patients hospitalized with AF and to explore the use of anticoagulation treatments in Han and Uygur patients in Xinjiang, northwest ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45884
更新日期：2017-04-10 00:00:00
abstract：:The motion of a laser-heated Janus particle is experimentally measured under a rotating electric field. Directionally circular motions of the Janus particle following or countering the direction of the rotating electric field are observed in the low-frequency region (from 1 to 6 kHz) depending on the direction of elec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24343-w
更新日期：2018-04-13 00:00:00
abstract：:The amount of reduction in opioid doses and its effect on postoperative pain outcomes in chronic kidney disease (CKD) patients in the perioperative setting remains unclear. This study aimed to investigate differences in postoperative pain outcomes after major laparoscopic surgery between patients with CKD and those wi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40627-1
更新日期：2019-03-08 00:00:00
abstract：:Drought is one of the major impediments in wheat productivity. Traditional breeding and marker assisted QTL introgression had limited success. Available wheat genomic and RNA-seq data can decipher novel drought tolerance mechanisms with putative candidate gene and marker discovery. Drought is first sensed by root tiss...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49915-2
更新日期：2019-09-26 00:00:00
abstract：:L-arginine:glycine amidinotransferase (AGAT) and its metabolites creatine and homoarginine (HA) have been linked to cardiovascular pathologies in both human and murine studies, but the underlying molecular mechanisms are poorly understood. Here, we report the first analysis of heart transcriptome variation using micro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61638-3
更新日期：2020-03-16 00:00:00
abstract：:Networks are a powerful abstraction with applicability to a variety of scientific fields. Models explaining their morphology and growth processes permit a wide range of phenomena to be more systematically analysed and understood. At the same time, creating such models is often challenging and requires insights that ma...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06284
更新日期：2014-09-05 00:00:00
abstract：:In this paper, we evaluated the power of microbiome measures taken at three time points over the growth test period (weaning, 15 and 22 weeks) to foretell growth and carcass traits in 1039 individuals of a line of crossbred pigs. We measured prediction accuracy as the correlation between actual and predicted phenotype...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43031-x
更新日期：2019-04-25 00:00:00
abstract：:Inter-digital capacitors (IDCs) with aerosol-deposition (AD) high-k dielectric layer were compared via simulation and measurements of bare IDCs and AD IDCs at room temperature and subjected to a post-annealing process for realizing capacitive super-sensing applications. IDCs with thin AD films can provide higher capac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37416-7
更新日期：2019-01-24 00:00:00
abstract：:Patients with asymptomatic occlusion in the internal carotid arteries (ICA) have been shown to have a better preserved hemodynamic status of the brain as compared to patients with symptoms. This study was aimed to explore the cerebral perfusion alterations in asymptomatic patients using multi-parametric arterial spin-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02094-4
更新日期：2017-05-12 00:00:00
abstract：:Recently, mesoporous carbon nitride (MCN) has aroused extensive interest for its potential applications in organocatalysis, photo- and electrochemistry and CO2 capture. However, further surface functionalization of MCN for advanced nanomaterials and catalysis still remains very challenging. Here we show that acidic ca...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12901
更新日期：2015-08-05 00:00:00
abstract：:The ability to accurately forecast seizures could significantly improve the quality of life of patients with drug-refractory epilepsy. Prediction capabilities rely on the adequate identification of seizure activity precursors from electroencephalography recordings. Although a long list of features has been proposed, n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33969-9
更新日期：2018-10-19 00:00:00
abstract：:Solute segregating to grain boundary can stabilize the microstructure of nanocrystalline materials, but a lot of solutes also cause embrittlement effect on interfacial strength. Therefore, uncovering the solute effect on grain boundary strength is very important for nanocrystalline alloys design. In this work, we have...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74065-1
更新日期：2020-10-08 00:00:00
abstract：:A robust method is presented for the segmentation of the full cerebral vasculature in 4-dimensional (4D) computed tomography (CT). The method consists of candidate vessel selection, feature extraction, random forest classification and postprocessing. Image features include among others the weighted temporal variance i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15617-w
更新日期：2017-11-15 00:00:00
abstract：:The Arabidopsis Tóxicos en Levadura (ATL) protein family is a class of E3 ubiquitin ligases with a characteristic RING-H2 Zn-finger structure that mediates diverse physiological processes and stress responses in plants. We carried out a genome-wide survey of grapevine (Vitis vinifera L.) ATL genes and retrieved 96 seq...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38260
更新日期：2016-12-02 00:00:00
abstract：:Bleaching, the loss of algal symbionts, occurs in marine photosymbiotic organisms at water temperatures minimally exceeding average summer SST (sea surface temperatures). Pre-adaptation allows organisms to persist under warmer conditions, providing the tolerance can be carried to new habitats. Here we provide evidence...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30930
更新日期：2016-08-09 00:00:00
abstract：:Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst. This study was to investigate the effect of asynchronous embryo transfer on uterine receptivity in mice. Embryos under different stages were transferred into two oviduct sides of a recipient mouse on day 1 o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15897
更新日期：2015-11-04 00:00:00
abstract：:Upper urinary tract urothelial carcinoma (UT-UC) is rare and treatment options or prognostic markers are limited. There is increasing evidence indicating that urothelial carcinoma may be an endocrine-related cancer. The aim of this study was to analyze the prognostic effect of estrogen receptor beta (ERβ) on the outco...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24263
更新日期：2016-04-07 00:00:00
abstract：:Modulation of photoluminescence of atomically thin transition metal dichalcogenide two-dimensional materials is critical for their integration in optoelectronic and photonic device applications. By coupling with different plasmonic array geometries, we have shown that the photoluminescence intensity can be enhanced an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41175
更新日期：2017-01-30 00:00:00
abstract：:Proteasomes are a critical component of quality control that regulate turnover of short-lived, unfolded, and misfolded proteins. Proteasome activity has been therapeutically targeted and considered as a treatment option for several chronic lung disorders including pulmonary fibrosis. Although pharmacologic inhibition ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49020-4
更新日期：2019-08-29 00:00:00
abstract：:The advent of random start protocols to shorten the time needed to store oocytes in women with malignancies has represented an important improvement in the field of fertility preservation. However, Randomized Controlled Trials are difficult to implement in this area and available evidence that supports this approach r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67151-x
更新日期：2020-06-22 00:00:00
abstract：:The association between gene polymorphisms and plasma virus load at the set point (SP-PVL) was investigated in Mauritian macaques inoculated with SIV. Among 44 macaques inoculated with 50 AID50, six individuals were selected: three with SP-PVL among the highest and three with SP-PVL among the lowest. The exons of 390 ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44235-x
更新日期：2019-05-28 00:00:00
abstract：:Our understanding of a variety of phenomena in physics, biology and economics crucially depends on the analysis of multivariate time series. While a wide range tools and techniques for time series analysis already exist, the increasing availability of massive data structures calls for new approaches for multidimension...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15508
更新日期：2015-10-21 00:00:00
abstract：:Cancer-specific promoter driven replication of oncolytic adenovirus (Ad) is cancer-specific, but shows low transcriptional activity. Thus, we generated several chimeric α-fetoprotein (AFP) promoter variants, containing reconstituted enhancer and silencer regions, to preferentially drive Ad replication in hepatocellula...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20268-6
更新日期：2018-02-02 00:00:00
abstract：:Application of PCR to multiplexing assays is not trivial; it requires multiple fluorescent labels for amplicon detection and sophisticated software for data interpretation. Alternative PCR-free methods exploiting new concepts in nanotechnology exhibit high sensitivities but require multiple labeling and/or amplificati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02033
更新日期：2013-01-01 00:00:00
abstract：:That stalks reorient after flowering to face upwards is a common phenomenon in many flowering plants, indicating the potential importance of fruit orientation on seed dispersal. But this idea has not been subject to an empirical test. We examined this hypothesis by analysing the evolutionary correlation between fruit ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20146
更新日期：2016-02-02 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-69653-0
更新日期：2020-07-24 00:00:00
abstract：:Our understanding of the structure and regulation of Plasmodium vivax genes is limited by our inability to grow the parasites in long-term in vitro cultures. Most P. vivax studies must therefore rely on patient samples, which typically display a low proportion of parasites and asynchronous parasites. Here, we present ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07275-9
更新日期：2017-08-10 00:00:00
abstract：:Diagnostic testing often assesses the cardiovascular or respiratory systems in isolation, ignoring the major pathophysiologic interactions between the systems in many diseases. When both systems are assessed currently, multiple modalities are utilized in costly fashion with burdensome logistics and decreased accessibi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04676-8
更新日期：2017-07-13 00:00:00
abstract：:Increasing human activity along the coast has amplified the extinction risk of inshore delphinids. Informed selection and prioritisation of areas for the conservation of inshore delphinids requires a comprehensive understanding of their distribution and habitat use. In this study, we applied an ensemble species distri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69863-6
更新日期：2020-09-01 00:00:00
abstract：:The high metabolic activity associated with endurance flights and intense fuelling of migrant birds may produce large quantities of reactive oxygen species, which cause oxidative damage. Yet it remains unknown how long-lived birds prepare for oxidative challenges prior to extreme flights. We combined blood measurement...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54057-6
更新日期：2019-11-26 00:00:00
