An appraisal of whole-room indirect calorimeters and a metabolic cart for measuring resting and active metabolic rates.

Abstract:

:Whole-room indirect calorimeters (WRICs) have traditionally been used for real-time resting metabolic rate (RMR) measurements, while metabolic rate (MR) during short-interval exercises has commonly been measured by metabolic carts (MCs). This study aims to investigate the feasibility of incorporating short-interval exercises into WRIC study protocols by comparing the performance of WRICs and an MC. We assessed the 40-min RMR of 15 subjects with 2-day repeats and the 10-15 min activity MR (AMR) of 14 subjects at three intensities, using a large WRIC, a small WRIC, and an MC. We evaluated the biases between the instruments and quantified sources of variation using variance component analysis. All three instruments showed good agreement for both RMR (maximum bias = 0.07 kcal/min) and AMR assessment (maximum bias = 0.53 kcal/min). Moreover, the majority of the variability was between-subject and between-intensity variation, whereas the types of instrument contributed only a small amount to total variation in RMR (2%) and AMR (0.2%) data. In Conclusion, the good reproducibility among the instruments indicates that they may be used interchangeably in well-designed studies. Overall, WRICs can serve as an accurate and versatile means of assessing MR, capable of integrating RMR and short-interval AMR assessments into a single protocol.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Chen S,Scott C,Pearce JV,Farrar JS,Evans RK,Celi FS

doi

10.1038/s41598-020-71001-1

subject

Has Abstract

pub_date

2020-08-31 00:00:00

pages

14343

issue

1

issn

2045-2322

pii

10.1038/s41598-020-71001-1

journal_volume

10

pub_type

杂志文章
  • Functional analysis of missense variants in the TRESK (KCNK18) K channel.

    abstract::A loss of function mutation in the TRESK K2P potassium channel (KCNK18), has recently been linked with typical familial migraine with aura. We now report the functional characterisation of additional TRESK channel missense variants identified in unrelated patients. Several variants either had no apparent functional ef...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep00237

    authors: Andres-Enguix I,Shang L,Stansfeld PJ,Morahan JM,Sansom MS,Lafrenière RG,Roy B,Griffiths LR,Rouleau GA,Ebers GC,Cader ZM,Tucker SJ

    更新日期:2012-01-01 00:00:00

  • Late p65 nuclear translocation in glioblastoma cells indicates non-canonical TLR4 signaling and activation of DNA repair genes.

    abstract::Glioblastoma (GBM) is the most aggressive brain primary malignancy. Toll-like receptor 4 (TLR4) has a dual role in cell fate, promoting cell survival or death depending on the context. Here, we analyzed TLR4 expression in different grades of astrocytoma, and observed increased expression in tumors, mainly in GBM, comp...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-79356-1

    authors: Moretti IF,Lerario AM,Trombetta-Lima M,Sola PR,da Silva Soares R,Oba-Shinjo SM,Marie SKN

    更新日期:2021-01-14 00:00:00

  • Immobilization of Polyoxometalate in the Metal-Organic Framework rht-MOF-1: Towards a Highly Effective Heterogeneous Catalyst and Dye Scavenger.

    abstract::A series of three remarkable complexes [PMo12O40]@[Cu6O(TZI)3(H2O)9]4·OH·31H2O (H3TZI = 5-tetrazolylisophthalic acid; denoted as HLJU-1, HLJU = Heilongjiang University), [SiMo12O40]@[Cu6O(TZI)3(H2O)9]4·32H2O (denoted as HLJU-2), and [PW12O40]@[Cu6O(TZI)3(H2O)6]4·OH·31H2O (denoted as HLJU-3) have been isolated by using...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep25595

    authors: Sun JW,Yan PF,An GH,Sha JQ,Li GM,Yang GY

    更新日期:2016-05-09 00:00:00

  • Ice-core evidence of earliest extensive copper metallurgy in the Andes 2700 years ago.

    abstract::The importance of metallurgy for social and economic development is indisputable. Although copper (Cu) was essential for the wealth of pre- and post-colonial societies in the Andes, the onset of extensive Cu metallurgy in South America is still debated. Comprehensive archaeological findings point to first sophisticate...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep41855

    authors: Eichler A,Gramlich G,Kellerhals T,Tobler L,Rehren T,Schwikowski M

    更新日期:2017-01-31 00:00:00

  • FR58P1a; a new uncoupler of OXPHOS that inhibits migration in triple-negative breast cancer cells via Sirt1/AMPK/β1-integrin pathway.

    abstract::Highly malignant triple-negative breast cancer (TNBC) cells rely mostly on glycolysis to maintain cellular homeostasis; however, mitochondria are still required for migration and metastasis. Taking advantage of the metabolic flexibility of TNBC MDA-MB-231 cells to generate subpopulations with glycolytic or oxidative p...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-31367-9

    authors: Urra FA,Muñoz F,Córdova-Delgado M,Ramírez MP,Peña-Ahumada B,Rios M,Cruz P,Ahumada-Castro U,Bustos G,Silva-Pavez E,Pulgar R,Morales D,Varela D,Millas-Vargas JP,Retamal E,Ramírez-Rodríguez O,Pessoa-Mahana H,Pavani M,Fer

    更新日期:2018-09-04 00:00:00

  • Laterals take it better - Emerging and young lateral roots survive lethal salinity longer than the primary root in Arabidopsis.

    abstract::Plant responses to salinity have been extensively studied over the last decades. Despite the vast accumulated knowledge, the ways Arabidopsis lateral roots (LR) cope with lethal salinity has not been fully resolved. Here we compared the primary root (PR) and the LR responses during events leading to lethal salinity (N...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60163-7

    authors: Ambastha V,Friedmann Y,Leshem Y

    更新日期:2020-02-24 00:00:00

  • Different extent in decline of infant mortality by region and cause in Shenyang, China.

    abstract::To compare the pattern of cause of death of infant mortality rates by urban/rural areas as well as to generate knowledge for potential strategies to decrease this mortality, we carried out a study by analyzing the infant mortality data from the Shenyang Women and Children Health Care Centre. From 1997 to 2014, 970,583...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep24527

    authors: Huang YH,Wu QJ,Li LL,Li D,Li J,Zhou C,Wu L,Zhu J,Gong TT

    更新日期:2016-04-14 00:00:00

  • Distribution and clinical associations of integrating conjugative elements and cag pathogenicity islands of Helicobacter pylori in Indonesia.

    abstract::The clinical associations and correlations with other virulence factors such as cag pathogenicity island (PAI) of the Integrating Conjugative Elements Helicobacter pylori TFSS (ICEHptfs), a new type IV secretion system (TFSS) in H. pylori has not been described. Among 103 studied strains from Indonesia, almost all str...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-24406-y

    authors: Waskito LA,Miftahussurur M,Lusida MI,Syam AF,Suzuki R,Subsomwong P,Uchida T,Hamdan M,Nasronudin,Yamaoka Y

    更新日期:2018-04-17 00:00:00

  • Three cooperative mechanisms required for recovery after brain damage.

    abstract::Stroke is one of the main causes of human disabilities. Experimental observations indicate that several mechanisms are activated during the recovery of functional activity after a stroke. Here we unveil how the brain recovers by explaining the role played by three mechanisms: Plastic adaptation, hyperexcitability and ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-50946-y

    authors: Berger D,Varriale E,van Kessenich LM,Herrmann HJ,de Arcangelis L

    更新日期:2019-11-01 00:00:00

  • Radiotherapy-Compatible Robotic System for Multi-Landmark Positioning in Head and Neck Cancer Treatments.

    abstract::The spine flexibility creates one of the most significant challenges to proper positioning in radiation therapy of head and neck cancers. Even though existing immobilization techniques can reduce the positioning uncertainty, residual errors (2-3 mm along the cervical spine) cannot be mitigated by single translation-ba...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-50797-7

    authors: Ostyn M,Wang S,Kim YS,Kim S,Yeo WH

    更新日期:2019-10-07 00:00:00

  • Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.

    abstract::The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves. To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey)...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-54360-2

    authors: Chen Y,Zhou J,Ren K,Zou C,Liu J,Yao G,He J,Zhao G,Huang W,Hu B,Chen Y,Xiong K,Jin Y

    更新日期:2019-11-28 00:00:00

  • Dipeptidyl Peptidase-4 Inhibitor Increases Vascular Leakage in Retina through VE-cadherin Phosphorylation.

    abstract::The inhibitors of CD26 (dipeptidyl peptidase-4; DPP4) have been widely prescribed to control glucose level in diabetic patients. DPP4-inhibitors, however, accumulate stromal cell-derived factor-1α (SDF-1α), a well-known inducer of vascular leakage and angiogenesis both of which are fundamental pathophysiology of diabe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29393

    authors: Lee CS,Kim YG,Cho HJ,Park J,Jeong H,Lee SE,Lee SP,Kang HJ,Kim HS

    更新日期:2016-07-06 00:00:00

  • Genetic diversity of PRRSV 1 in Central Eastern Europe in 1994-2014: origin and evolution of the virus in the region.

    abstract::More than 20 years after the first outbreaks, the phylogenetic picture of PRRSV is still incomplete and full of gaps, especially in regards of PRRSV 1. Due to the exceptional diversity observed at the eastern borders of Europe and the low number of available sequences from Central Eastern European countries, the autho...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-26036-w

    authors: Balka G,Podgórska K,Brar MS,Bálint Á,Cadar D,Celer V,Dénes L,Dirbakova Z,Jedryczko A,Márton L,Novosel D,Petrović T,Sirakov I,Szalay D,Toplak I,Leung FC,Stadejek T

    更新日期:2018-05-17 00:00:00

  • Cromolyn sodium delays disease onset and is neuroprotective in the SOD1G93A Mouse Model of amyotrophic lateral sclerosis.

    abstract::Accumulating evidence suggests that neuroinflammatory processes are implicated in the initiation and progression of amyotrophic lateral sclerosis (ALS). Previous reports have demonstrated an increase in microgliosis and astrogliosis in the lumbar spinal cord of SOD1G93A transgenic mice before the onset of symptoms, a ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-53982-w

    authors: Granucci EJ,Griciuc A,Mueller KA,Mills AN,Le H,Dios AM,McGinty D,Pereira J,Elmaleh D,Berry JD,Paganoni S,Cudkowicz ME,Tanzi RE,Sadri-Vakili G

    更新日期:2019-11-27 00:00:00

  • Extracellular cholesterol oxidase production by Streptomyces aegyptia, in vitro anticancer activities against rhabdomyosarcoma, breast cancer cell-lines and in vivo apoptosis.

    abstract::In recent years, microbial cholesterol oxidases have gained great attention due to its widespread use in medical applications for serum cholesterol determination. Streptomyces aegyptia strain NEAE-102 exhibited high level of extracellular cholesterol oxidase production using a minimum medium containing cholesterol as ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-20786-3

    authors: El-Naggar NE,Soliman HM,El-Shweihy NM

    更新日期:2018-02-09 00:00:00

  • Abundance and nuclear antigen reactivity of intestinal and fecal Immunoglobulin A in lupus-prone mice at younger ages correlate with the onset of eventual systemic autoimmunity.

    abstract::Our recent studies, using (SWRxNZB)F1 (SNF1) mice, showed a potential contribution of the gut microbiota and pro-inflammatory immune responses of the gut mucosa to systemic autoimmunity in lupus. Here, using this mouse model, we determined the abundance and the nAg reactivity of IgA antibody produced in the intestine ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-71272-8

    authors: Sun W,Gudi RR,Johnson BM,Vasu C

    更新日期:2020-08-31 00:00:00

  • Fossilized Melts in Mantle Wedge Peridotites.

    abstract::The shallow oxidized asthenosphere may contain a small fraction of potassic silicate melts that are enriched in incompatible trace elements and volatiles. Here, to determine the chemical composition of such melt, we analysed fossilized melt inclusions, preserved as multiphase solid inclusions, from an orogenic garnet ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-28264-6

    authors: Naemura K,Hirajima T,Svojtka M,Shimizu I,Iizuka T

    更新日期:2018-07-04 00:00:00

  • Evaluation of Macaca radiata as a non-human primate model of Dengue virus infection.

    abstract::Dengue virus (DENV) causes a wide range of illnesses in humans, including dengue fever and dengue haemorrhagic fever. Current animal models of DENV infection are limited for understanding infectious diseases in humans. Bonnet monkeys (Macaca radiata), a type of Old World monkey, have been used to study experimental an...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21582-9

    authors: Kato F,Ishida Y,Kawakami A,Takasaki T,Saijo M,Miura T,Hishiki T

    更新日期:2018-02-21 00:00:00

  • Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins.

    abstract::Serratia marcescens is one of the important nosocomial pathogens which rely on quorum sensing (QS) to regulate the production of biofilm and several virulence factors. Hence, blocking of QS has become a promising approach to quench the virulence of S. marcescens. For the first time, QS inhibitory (QSI) and antibiofilm...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-16507-x

    authors: Sethupathy S,Ananthi S,Selvaraj A,Shanmuganathan B,Vigneshwari L,Balamurugan K,Mahalingam S,Pandian SK

    更新日期:2017-11-27 00:00:00

  • Transcriptomic Analysis of Aedes aegypti in Response to Mosquitocidal Bacillus thuringiensis LLP29 Toxin.

    abstract::Globally, Aedes aegypti is one of the most dangerous mosquitoes that plays a crucial role as a vector for human diseases, such as yellow fever, dengue, and chikungunya. To identify (1) transcriptomic basis of midgut (2) key genes that are involved in the toxicity process by a comparative transcriptomic analysis betwee...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-30741-x

    authors: Batool K,Alam I,Wu S,Liu W,Zhao G,Chen M,Wang J,Xu J,Huang T,Pan X,Yu X,Guan X,Xu L,Zhang L

    更新日期:2018-08-23 00:00:00

  • Increased blood COASY DNA methylation levels a potential biomarker for early pathology of Alzheimer's disease.

    abstract::Early diagnosis of dementia including Alzheimer's disease (AD) is an urgent medical and welfare issue. However, to date, no simple biometrics have been available. We reported that blood DNA methylation levels of the COASY gene, which encodes coenzyme A synthase, were increased in individuals with AD and amnestic mild ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-69248-9

    authors: Kobayashi N,Shinagawa S,Niimura H,Kida H,Nagata T,Tagai K,Shimada K,Oka N,Shikimoto R,Noda Y,Nakajima S,Mimura M,Shigeta M,Kondo K

    更新日期:2020-07-22 00:00:00

  • Collision of millimetre droplets induces DNA and protein transfection into cells.

    abstract::Nonperturbing and simple transfection methods are important for modern techniques used in biotechnology. Recently, we reported that electrospraying can be applied to DNA transfection in cell lines, bacteria, and chicken embryos. However, the transfection efficiency was only about 2%. To improve the transfection rate, ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep00289

    authors: Ikemoto K,Sakata I,Sakai T

    更新日期:2012-01-01 00:00:00

  • Qubit lattice coherence induced by electromagnetic pulses in superconducting metamaterials.

    abstract::Quantum bits (qubits) are at the heart of quantum information processing schemes. Currently, solid-state qubits, and in particular the superconducting ones, seem to satisfy the requirements for being the building blocks of viable quantum computers, since they exhibit relatively long coherence times, extremely low diss...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29374

    authors: Ivić Z,Lazarides N,Tsironis GP

    更新日期:2016-07-12 00:00:00

  • Surface and Bulk Carbide Transformations in High-Speed Steel.

    abstract::We have studied the transformation of carbides in AISI M42 high-speed steels in the temperature window used for forging. The annealing was found to result in the partial transformation of the large, metastable M2C carbides into small, more stable grains of M6C, with an associated change in the crystal orientation. In ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep16202

    authors: Godec M,Večko Pirtovšek T,Šetina Batič B,McGuiness P,Burja J,Podgornik B

    更新日期:2015-11-05 00:00:00

  • Dynamic features of water molecules in superconcentrated aqueous electrolytes.

    abstract::An existence of ions dissolved in water has significant effects on bulk properties of water. Superconcentrated conditions have been recently proposed to provide a new concept of lithium ion batteries in order to overcome limitations for practical applications. In those conditions, water would undergo significant chang...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-27706-5

    authors: Han S

    更新日期:2018-06-19 00:00:00

  • Inhibition of protein aggregation by zwitterionic polymer-based core-shell nanogels.

    abstract::Protein aggregation is a process by which misfolded proteins polymerizes into aggregates and forms fibrous structures with a β-sheet conformation, known as amyloids. It is an undesired outcome, as it not only causes numerous neurodegenerative diseases, but is also a major deterrent in the development of protein biopha...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep45777

    authors: Rajan R,Matsumura K

    更新日期:2017-04-04 00:00:00

  • Subcellular localization of the sigma-1 receptor in retinal neurons - an electron microscopy study.

    abstract::The Sigma-1 receptor (S1R) is known to play a protective role in the central nervous system including the retina. A major barrier for understanding the underlying mechanism is an ambiguity of S1R subcellular localizations. We thus conducted the first electron microscopy (EM) study of S1R subcellular distribution in th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep10689

    authors: Mavlyutov TA,Epstein M,Guo LW

    更新日期:2015-06-02 00:00:00

  • Fully printed flexible and disposable wireless cyclic voltammetry tag.

    abstract::A disposable cyclic voltammetry (CV) tag is printed on a plastic film by integrating wireless power transmitter, polarized triangle wave generator, electrochemical cell and signage through a scalable gravure printing method. By proximity of 13.56 MHz RF reader, the printed CV tag generates 320 mHz of triangular sweep ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08105

    authors: Jung Y,Park H,Park JA,Noh J,Choi Y,Jung M,Jung K,Pyo M,Chen K,Javey A,Cho G

    更新日期:2015-01-29 00:00:00

  • Influence of tunnel ionization to third-harmonic generation of infrared femtosecond laser pulses in air.

    abstract::Here we present an experimental as well as theoretical study of third-harmonic generation in tightly focused femtosecond filaments in air at the wavelength of [Formula: see text]. At low intensities, longitudinal phase matching is dominating in the formation of 3rd harmonics, whereas at higher intensities locked X-wav...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74263-x

    authors: Tamulienė V,Juškevičiūtė G,Buožius D,Vaičaitis V,Babushkin I,Morgner U

    更新日期:2020-10-15 00:00:00

  • A novel metabarcoding diagnostic tool to explore protozoan haemoparasite diversity in mammals: a proof-of-concept study using canines from the tropics.

    abstract::Haemoparasites are responsible for some of the most prevalent and debilitating canine illnesses across the globe, whilst also posing a significant zoonotic risk to humankind. Nowhere are the effects of such parasites more pronounced than in developing countries in the tropics where the abundance and diversity of ectop...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-49118-9

    authors: Huggins LG,Koehler AV,Ng-Nguyen D,Wilcox S,Schunack B,Inpankaew T,Traub RJ

    更新日期:2019-09-02 00:00:00