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Collision of millimetre droplets induces DNA and protein transfection into cells.

Abstract：

:Nonperturbing and simple transfection methods are important for modern techniques used in biotechnology. Recently, we reported that electrospraying can be applied to DNA transfection in cell lines, bacteria, and chicken embryos. However, the transfection efficiency was only about 2%. To improve the transfection rate, physical properties of the sprayed droplets were studied in different variations of the method. We describe a highly efficient technique (30-93%) for introduction of materials such as DNA and protein into living cells by electrospraying droplets of a high conductivity liquid onto cells incubated with the material for transfection. Electric conductivity has a sizable influence on the success of transfection. In contrast, molecular weight of the transfected material, types of ions in the electrospray solution, and the osmotic pressure do not influence transfection efficiency. The physical analysis revealed that collision of cells with millimetre-sized droplets activates intracellular uptake.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Ikemoto K,Sakata I,Sakai T

doi

10.1038/srep00289

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

289

issn

2045-2322

journal_volume

2

pub_type

杂志文章

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