Abstract：
:Two-dimensional (2D) cell cultures growing on plastic do not recapitulate the three dimensional (3D) architecture and complexity of human tumors. More representative models are required for drug discovery and validation. Here, 2D culture and 3D mono- and stromal co-culture models of increasing complexity have been established and cross-comparisons made using three standard cell carcinoma lines: MCF7, LNCaP, NCI-H1437. Fluorescence-based growth curves, 3D image analysis, immunohistochemistry and treatment responses showed that end points differed according to cell type, stromal co-culture and culture format. The adaptable methodologies described here should guide the choice of appropriate simple and complex in vitro models.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Stock K,Estrada MF,Vidic S,Gjerde K,Rudisch A,Santo VE,Barbier M,Blom S,Arundkar SC,Selvam I,Osswald A,Stein Y,Gruenewald S,Brito C,van Weerden W,Rotter V,Boghaert E,Oren M,Sommergruber W,Chong Y,de Hoogt R,Graedoi
10.1038/srep28951subject
Has Abstractpub_date
2016-07-01 00:00:00pages
28951issn
2045-2322pii
srep28951journal_volume
6pub_type
杂志文章abstract：:Vitiligo is an acquired depigmentation disorder largely caused by defective melanocyte- or autoimmunity-induced melanocyte destruction. The aryl hydrocarbon receptor (AHR) is essential for melanocyte homeostasis and immune process, and abnormal AHR was observed in vitiligo. We previously identified the T allele of AHR...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13542
更新日期：2015-09-15 00:00:00
abstract：:Only few applications are currently dealing with personalized adverse drug reactions (ADRs) prediction in case of polypharmacy. The study aimed to develop a patient-tailored ADR web application, considering characteristics from 734 drugs and relevant patient related factors. The application was designed in Python usin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66611-8
更新日期：2020-06-12 00:00:00
abstract：:The expansion of agriculture is shrinking pristine forest areas worldwide, jeopardizing the persistence of their wild inhabitants. The Udzungwa red colobus monkey (Procolobus gordonorum) is among the most threatened primate species in Africa. Primarily arboreal and highly sensitive to hunting and habitat destruction, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14862
更新日期：2015-10-07 00:00:00
abstract：:Pyrene-conjugated compounds are detected by label-assisted laser desorption/ionization mass spectrometry (LA-LDI MS) without matrixes. We found that 6-amidopyrene derivatives were highly detectable by the LDI MS instrument equipped with a 355 nm laser. In a certain case of a 6-amidopyrene derivative, a molecular ion p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17853
更新日期：2015-12-15 00:00:00
abstract：:Metabolomics has emerged as a powerful approach to comprehensively interrogate cellular biochemistry. As such, we applied an untargeted liquid chromatography-mass spectrometry metabolomic strategy to elucidate metabolome changes in the anthracnose-causing hemibiotrophic sorghum pathogen, Colletotrichum sublineolum. An...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38692-7
更新日期：2019-03-01 00:00:00
abstract：:In arthropods, retinoid X receptor (RXR) is a highly conserved nuclear hormone receptor. By forming a heterodimeric complex with the ecdysone receptor (EcR), RXR is known to be vital importance for various physiological processes. However, in comparison to EcR, the RXR signaling pathway and its roles in crustacean rep...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-03-24 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29283
更新日期：2016-07-04 00:00:00
abstract：:Human induced pluripotent stem cells (hiPSCs) represent an almost limitless source of cells for disease modelling and drug screening applications. Here we established an efficient and robust 3D platform for cardiomyocyte (CMs) production from hiPSCs, solely through small-molecule-based temporal modulation of the Wnt s...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-06-25 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-04-16 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-09-12 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-01-23 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-08-31 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09393-w
更新日期：2017-08-22 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-10-29 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-57184-2
更新日期：2020-01-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02746
更新日期：2013-09-25 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51176-y
更新日期：2019-10-10 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-06-30 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-03-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-11-08 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40619
更新日期：2017-01-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51708-6
更新日期：2019-10-23 00:00:00
abstract：:Non-homologous end joining (NHEJ) is a major DNA double-strand break (DSB) repair mechanism. We characterized here a series of plasmid-based DSB templates that were repaired in Xenopus egg extracts via the canonical, Ku-dependent NHEJ pathway. We showed that the template with compatible ends was efficiently repaired w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27797
更新日期：2016-06-21 00:00:00
abstract：:The transcriptional responses of yeast cells to diverse stresses typically include gene activation and repression. Specific stress defense, citric acid cycle and oxidative phosphorylation genes are activated, whereas protein synthesis genes are coordinately repressed. This view was achieved from comparative transcript...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26170-5
更新日期：2018-05-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-07-22 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43006-y
更新日期：2019-04-29 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04485
更新日期：2014-03-27 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11520
更新日期：2015-07-09 00:00:00
abstract：:Species of Pomacea, commonly known as apple snails, are native to South America, and have become widely distributed agricultural and environmental pests in southern China since their introduction in the 1980s. However, only since 2010 have researchers recognized that at least two species, P. canaliculata and P. macula...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-19000-7
更新日期：2018-01-19 00:00:00
