Abstract：
:Non-homologous end joining (NHEJ) is a major DNA double-strand break (DSB) repair mechanism. We characterized here a series of plasmid-based DSB templates that were repaired in Xenopus egg extracts via the canonical, Ku-dependent NHEJ pathway. We showed that the template with compatible ends was efficiently repaired without end processing, in a manner that required the kinase activity of DNA-PKcs but not ATM. Moreover, non-compatible ends with blunt/3'-overhang, blunt/5'-overhang, and 3'-overhang/5'-overhang were predominantly repaired with fill-in and ligation without the removal of end nucleotides. In contrast, 3'-overhang/3'-overhang and 5'-overhang/5'-overhang templates were processed by resection of 3-5 bases and fill-in of 1-4 bases prior to end ligation. Therefore, the NHEJ machinery exhibited a strong preference for precise repair; the presence of neither non-compatible ends nor protruding single strand DNA sufficiently warranted the action of nucleases. ATM was required for the efficient repair of all non-compatible ends including those repaired without end processing by nucleases, suggesting its role beyond phosphorylation and regulation of Artemis. Finally, dephosphorylation of the 5'-overhang/3'-overhang template reduced the efficiency of DNA repair without increasing the risk of end resection, indicating that end protection via prompt end ligation is not the sole mechanism that suppresses the action of nucleases.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zhu S,Peng Adoi
10.1038/srep27797subject
Has Abstractpub_date
2016-06-21 00:00:00pages
27797issn
2045-2322pii
srep27797journal_volume
6pub_type
杂志文章abstract：:Increased ultraviolet B (UVB) radiation due to global change can affect plant growth and metabolism. Here, we evaluated the capacity of quinoa to resist under short acute UVB irradiation. Quinoa was daily exposed for 30 or 60 min to 1.69 W m-2 UVB. The results showed that 30 min exposure in 9 d-course did not cause se...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18710-2
更新日期：2018-01-10 00:00:00
abstract：:We have analyzed the compositions of boron-carbon system, in which the [Formula: see text] compound is identified as structural stability at high pressure. The first-principles calculation is used to identify the phase diagram, electronic structure, and superconductivity of [Formula: see text]. Our results have demons...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-10-22 00:00:00
abstract：:Regulatory T cells (Tregs) expand in peripheral lymphoid organs and can produce immunosuppressive cytokines to support tumor growth. IL-10 abrogation efficiently induces Treg formation but dampens tumoral neuropilin-1 (Nrp-1) Treg signaling, which simultaneously augments Th1 and Th17 immunity. These effects are associ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24249
更新日期：2016-04-14 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-01-29 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
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更新日期：2020-06-23 00:00:00
abstract：:Thiamine monophosphate kinase (ThiL) catalyzes the last step of thiamine pyrophosphate (TPP) synthesis, the ATP-dependent phosphorylation of thiamine monophosphate (TMP) to thiamine pyrophosphate. We solved the structure of ThiL from the human pathogen A. baumanii in complex with a pair of substrates TMP and a non-hyd...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40558-x
更新日期：2019-03-13 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41108-1
更新日期：2019-03-18 00:00:00
abstract：:In this article we advance a cutting-edge methodology for the study of the dynamics of plant movements of nutation. Our approach, unlike customary kinematic analyses of shape, period, or amplitude, is based on three typical signatures of adaptively controlled processes and motions, as reported in the biological and be...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-11-10 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07334
更新日期：2014-12-05 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05519
更新日期：2014-07-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-04-14 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-08-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19729
更新日期：2016-01-28 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-11-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-02-12 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43700
更新日期：2017-03-03 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43435
更新日期：2017-03-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01678
更新日期：2013-01-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01873
更新日期：2013-01-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43555-2
更新日期：2019-05-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25440
更新日期：2016-05-05 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-07-02 00:00:00
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journal_title：Scientific reports
pub_type： 临床试验,杂志文章,多中心研究
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更新日期：2018-10-18 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06030
更新日期：2014-08-12 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48029-z
更新日期：2019-08-13 00:00:00
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journal_title：Scientific reports
pub_type： 已发布勘误
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更新日期：2018-05-22 00:00:00
abstract：:Curcumin is an important bioactive component of turmeric and also one of the important natural products, which has been investigated extensively. The precise mode of action of curcumin and its impact on system level protein networks are still not well studied. To identify the curcumin governed regulatory action on pro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69011-0
更新日期：2020-07-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43036-6
更新日期：2019-04-30 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27680
更新日期：2016-06-10 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33935-5
更新日期：2018-11-01 00:00:00
