Abstract:
:The absence of Ataxia-Telangiectasia mutated protein kinase (ATM) is associated with neurological, metabolic and cardiovascular defects. The protein has been associated with mitochondria and its absence results in mitochondrial dysfunction. Furthermore, it can be activated in the cytosol by mitochondrial oxidative stress and mediates a cellular anti-oxidant response through the pentose phosphate pathway (PPP). However, the precise location and function of ATM within mitochondria and its role in oxidative phosphorylation is still unknown. We show that ATM is found endogenously within cardiac myocyte mitochondria under normoxic conditions and is consistently associated with the inner mitochondrial membrane. Acute ex vivo inhibition of ATM protein kinase significantly decreased mitochondrial electron transfer chain complex I-mediated oxidative phosphorylation rate but did not decrease coupling efficiency or oxygen consumption rate during β-oxidation. Chemical inhibition of ATM in rat cardiomyoblast cells (H9c2) significantly decreased the excited-state autofluorescence lifetime of enzyme-bound reduced NADH and its phosphorylated form, NADPH (NAD(P)H; 2.77 ± 0.26 ns compared to 2.57 ± 0.14 ns in KU60019-treated cells). This suggests an interaction between ATM and the electron transfer chain in the mitochondria, and hence may have an important role in oxidative phosphorylation in terminally differentiated cells such as cardiomyocytes.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Blignaut M,Loos B,Botchway SW,Parker AW,Huisamen Bdoi
10.1038/s41598-019-41108-1subject
Has Abstractpub_date
2019-03-18 00:00:00pages
4782issue
1issn
2045-2322pii
10.1038/s41598-019-41108-1journal_volume
9pub_type
杂志文章abstract::Small ruminant lentiviruses (SRLVs) are found in sheep in Germany and Iran. SRLVs have been classified into four genotypes: A-C and E. Genotype A has been subdivided into 20 subtypes. Previous studies suggested that, first, the ancestors of genotype A are those SRLVs found in Turkey, second, the evolution of SRLVs is ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58990-9
更新日期:2020-02-10 00:00:00
abstract::High Refractive Index (HRI) dielectric nanoparticles have been proposed as an alternative to metallic ones due to their low absorption and magnetodielectric response in the VIS and NIR ranges. For the latter, important scattering directionality effects can be obtained. Also, systems constituted by dimers of HRI dielec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11401-y
更新日期:2017-09-11 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) is a devastating cause of diarrhea in pigs worldwide. Most of studies have focused on molecular and pathogenic characterization of PEDV, whereas there were limited studies in understanding the role of gut microbiota (GM) in viral-associated diarrhea. Here, using the Illumina MiSe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17830-z
更新日期:2017-12-12 00:00:00
abstract::While considered an extracellular pathogen, Pseudomonas aeruginosa has been reported to be engulfed by macrophages in cellular and animal models. However, the role of macrophages in P. aeruginosa clearance in vivo remains poorly studied. The major outer membrane porin OprF has been recently shown to be involved in P. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79678-0
更新日期:2021-01-11 00:00:00
abstract::Chromosomal toxin-antitoxin (TA) systems are widespread genetic elements among bacteria, yet, despite extensive studies in the last decade, their biological importance remains ambivalent. The ability of TA-encoded toxins to affect stress tolerance when overexpressed supports the hypothesis of TA systems being associat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65504-0
更新日期:2020-06-08 00:00:00
abstract::Although the power conversion efficiency (PCE) of perovskite solar cells (PSCs) reached up to 23%, their short lifetime and fast degradation still remain as the main challenges. In this work, a new facile optical method based on the high power UV-irradiation is presented for the recovery of the degraded PSCs. Addition...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45756-1
更新日期:2019-07-01 00:00:00
abstract::The accumulation of single nucleotide polymorphisms (SNPs) in the displacement loop (D-loop) of mitochondrial DNA (mtDNA) has been described in various types of cancers, and their association with cancer risk and disease outcome has been extensively identified. In the present study, we investigated the association bet...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02408
更新日期:2013-01-01 00:00:00
abstract::The glycocalyx regulates the interaction of mammalian cells with extracellular molecules, such as cytokines. However, it is unknown to which extend the glycocalyx of distinct cancer cells control the binding and uptake of nanoparticles. In the present study, exome sequencing data of cancer patients and analysis of dis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79882-y
更新日期:2020-12-31 00:00:00
abstract::We aimed to investigate the changes of olfaction of major depressive disorder (MDD) before and after medical treatment, and to preliminarily scrutinize the association between the olfactory function and the severity of depressive symptoms, response inhibition, and emotional responding. Forty-eight medicine-naïve MDD p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63416-7
更新日期:2020-04-14 00:00:00
abstract::Adding fluoride into bioactive glasses leads to fluorapatite formation and a decrease in glass transition temperature. Recently, chloride has been introduced into glasses as an alternative to fluoride. The presence of the large chloride ion lowers glass crystallisation tendency and increases glass molar volume, which ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19544-2
更新日期:2018-01-22 00:00:00
abstract::Surface plasmon resonators can drastically redistribute incident light over different output wave vectors and polarizations. This can lead for instance to sub-diffraction sized nanoapertures in metal films that beam and to nanoparticle antennas that enable efficient conversion of photons between spatial modes, or heli...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09966
更新日期:2015-04-30 00:00:00
abstract::The dermal striated muscle panniculus carnosus (PC), prevalent in lower mammals with remnants in humans, is highly regenerative, and whose function is purported to be linked to defence and shivering thermogenesis. Given the heterogeneity of responses of different muscles to disease, we set out to characterize the PC i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52071-2
更新日期:2019-11-04 00:00:00
abstract::Anxiety and depression are commonly found in patients with diabetes, but little is known about how the anxiety and depression symptoms of diabetes patients and the health-related quality of life (HRQoL) over time influence each other. Therefore, we conducted a survey among patients with diabetes (T1) and repeated the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57647-x
更新日期:2020-05-20 00:00:00
abstract::Vibrio vulnificus causes fatal infections in humans, and antibiotics are commonly used in treatment regimens against V. vulnificus infection. However, the therapeutic effects of antibiotics are limited by multidrug resistance. In this study, we demonstrated that an antimicrobial peptide (AMP), HPA3PHis, loaded onto a ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14127-z
更新日期:2017-10-19 00:00:00
abstract::In a developing nervous system, endogenous electric field (EF) influence embryonic growth. We reported the EF-directed migration of both rat Schwann cells (SCs) and oligodendrocyte precursor cells (OPCs) and explored the molecular mechanism using RNA-sequencing assay. However, previous studies revealed the differentia...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74085-x
更新日期:2020-10-08 00:00:00
abstract::No validated screening method currently exists for Chronic Obstructive Pulmonary Disease (COPD) in smokers. Therefore, we constructed a predictive model with simple parameters that can be applied for COPD screening to detect fixed airflow limitation. This observational cross-sectional study included a random sample of...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-018-31198-8
更新日期:2018-09-06 00:00:00
abstract::We investigated the extent to which pre-pregnancy obesity mediates the association between maternal place of birth and severe pre-eclampsia in the PreCARE cohort of pregnant women in Paris (n = 9,579). Adjusted path analysis logistic regression models were used to assess the role of pre-pregnancy obesity as a mediator...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究
doi:10.1038/s41598-020-62032-9
更新日期:2020-03-23 00:00:00
abstract::Given that the biological processes governing the oncogenesis of pancreatic cancers could present useful therapeutic targets, there is a pressing need to molecularly distinguish between different clinically relevant pancreatic cancer subtypes. To address this challenge, we used targeted proteomics and other molecular ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58290-2
更新日期:2020-01-27 00:00:00
abstract::The current economics of the fish protein industry demand rapid, accurate and expressive prediction algorithms at every step of protein production especially with the challenge of global climate change. This help to predict and analyze functional and nutritional quality then consequently control food allergies in hype...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10890-1
更新日期:2017-09-07 00:00:00
abstract::Significant aggregation between ZnO nanoparticles (ZnO NPs) dispersed in polar and nonpolar solvents hinders the formation of high quality thin film for the device application and impedes their excellent electron transporting ability. Herein a bifunctional coordination complex, titanium diisopropoxide bis(acetylaceton...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75070-0
更新日期:2020-10-22 00:00:00
abstract::We previously identified novel bioactive polysaccharides from Bactrocera cucurbitae and Antheraea yamamai that activate innate immunity in RAW264 murine macrophages. However, in terms of potential applications in the cultivation of prawns, there were problems with the availability of these insects. However, we have no...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27241-3
更新日期:2018-06-11 00:00:00
abstract::Innate immunity has been extended to respond environmental pathogen other than microbial components. Here we explore a novel pollen/TLR4 innate immunity in allergic inflammation. In experimental allergic conjunctivitis induced by short ragweed (SRW) pollen, typical allergic signs, stimulated IL-33/ST2 signaling and ov...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36150
更新日期:2016-10-31 00:00:00
abstract::Differentiating recurrent brain tumor from radiation necrosis is often difficult. This study aims to investigate the efficacy of 11C-methionine (MET)-PET radiomics for distinguishing recurrent brain tumor from radiation necrosis, as compared with conventional tumor-to-normal cortex (T/N) ratio evaluation. We enrolled ...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-019-52279-2
更新日期:2019-10-30 00:00:00
abstract::Calotropis gigantea (C. gigantea) extract with an ecofriendly nanotechnology approach could provide promising antimicrobial activity against skin pathogens. This study investigates the antimicrobial capability of green synthesized binary ZnO-CuO nanocomposites from C. gigantea against non-MDR (Staphylococcus aureus an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79547-w
更新日期:2021-01-08 00:00:00
abstract::Ultracentrifugationon sucrose density gradientappears to be the best purification protocol for extracellular vesicle (EVs) purification. After this step, to reduce disulfide bridges linking exogenous proteins to the vesicles, the collected samples are routinely washed and treated with dithiothreitol (DTT). Such incuba...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47598-3
更新日期:2019-09-10 00:00:00
abstract::Although blood-based liquid biopsies have emerged as a promising non-invasive method to detect biomarkers in various cancers, limited progress has been made for brain tumors. One major obstacle is the blood-brain barrier (BBB), which hinders efficient passage of tumor biomarkers into the peripheral circulation. The ob...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24516-7
更新日期:2018-04-26 00:00:00
abstract::We report on neutron diffraction, thermal expansion, magnetostriction, dielectric, and specific heat measurements on polycrystalline FeCr2S4 in external magnetic fields. The ferrimagnetic ordering temperatures TC ≈ 170 K and the transition at TOO ≈ 10 K, which has been associated with orbital ordering, are only weakly...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06079
更新日期:2014-08-15 00:00:00
abstract::It has been reported that in patients with operable stage I non-small cell lung cancer (NSCLC), overall survival (OS) is better in those who undergo hypofractionated stereotactic radiation therapy (HSRT) than in those who undergo surgery. However, the reason that HSRT has a better OS has not been fully explored. Here,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04978-x
更新日期:2017-07-07 00:00:00
abstract::Identifying patients at risk of poor outcome after mild traumatic brain injury (MTBI) is essential to aid prognostics and treatment. Diffuse axonal injury (DAI) may be the primary pathologic feature of MTBI but is normally not detectable by conventional imaging technology. This lack of sensitivity of clinical imaging ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13628-1
更新日期:2017-10-23 00:00:00
abstract::Arthroplasty is a surgical procedure to restore the function of the joint of patient suffering from knee osteoarthritis. However, postoperative functional deficits are reported even after a rehabilitation program. In order to determine the origin of functional deficits of patient suffering from knee osteoarthritis and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62146-0
更新日期:2020-03-24 00:00:00