Abstract：
:Pyrene-conjugated compounds are detected by label-assisted laser desorption/ionization mass spectrometry (LA-LDI MS) without matrixes. We found that 6-amidopyrene derivatives were highly detectable by the LDI MS instrument equipped with a 355 nm laser. In a certain case of a 6-amidopyrene derivative, a molecular ion peak [M](+•) and a characteristic fragment ion peak [M-42](+•) were detected in an amount of only 10 fmol. The latter peak, corresponding to the 6-aminopyrene fragment, might be generated in situ by the removal of ketene (CH2=C=O) from the parent molecule. A photoaffinity amidopyrene derivative of an antitumor macrolide aplyronine A (ApA-PaP) was synthesized, which showed potent cytotoxicity and actin-depolymerizing activity. In an LDI MS analysis of the MeOH- and water-adducts of ApA-PaP, oxime N-O bonds as well as amidopyrene N-acetyl moieties were preferentially cleaved, and their internal structures were confirmed by MS/MS analysis. Amidopyrene moiety might enhance fragmentation and stabilize the cleaved fragments by intramolecular or intermolecular weak interactions including hydrogen bonding. Our chemical probe methods might contribute to a detailed analysis of binding modes between various ligands and target biomacromolecules that include multiple and weak interactions.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Yoneda K,Hu Y,Kita M,Kigoshi Hdoi
10.1038/srep17853subject
Has Abstractpub_date
2015-12-15 00:00:00pages
17853issn
2045-2322pii
srep17853journal_volume
5pub_type
杂志文章abstract：:Magnesium-based thermoelectric materials (Mg2X, X = Si, Ge, Sn) have received considerable attention due to their availability, low toxicity, and reasonably good thermoelectric performance. The synthesis of these materials with high purity is challenging, however, due to the reactive nature and high vapour pressure of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04348-7
更新日期：2017-06-21 00:00:00
abstract：:Mortality of glioblastoma multiforme (GBM) has not improved over the last two decades despite medical breakthroughs in the treatment of other types of cancers. Nanoparticles hold tremendous promise to overcome the pharmacokinetic challenges and off-target adverse effects. However, an inhibitory effect of nanoparticles...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59146-5
更新日期：2020-02-11 00:00:00
abstract：:Glomerular permeability and subsequent albuminuria are early clinical markers for glomerular injury in hypertensive nephropathy. Albuminuria predicts mortality and cardiovascular morbidity. AT1 receptor blockers protect from albuminuria, cardiovascular morbidity and mortality. A blood pressure independent, molecular m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39513
更新日期：2016-12-22 00:00:00
abstract：:High mortality and dependent living after hip fracture pose a significant public health concern. Retrospective study was conducted with 481 hip fracture patients (≥65 years of age) undergoing surgery from March 2009 to May 2014. The Hip-MFS was calculated by Comprehensive Geriatric Assessment (CGA). The primary outcom...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42966
更新日期：2017-02-24 00:00:00
abstract：:Based on a hybrid pumping method consisting of a 1150 nm continuous-wave pump source and a 1950 nm pulsed pump source, we demonstrate a power controllable gain-switched fiber laser in dual wavebands at ~ 3 μm and ~ 2.1 μm. Different pumping schemes for pumping a Ho3+-doped ZBLAN fiber are studied. Using only the 1950 ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80238-9
更新日期：2021-01-13 00:00:00
abstract：:Malignant peripheral nerve sheath tumors (MPNST) are aggressive cancers that occur spontaneously (sporadic MPNST) or from benign plexiform neurofibromas in neurofibromatosis type 1 (NF1) patients. MPNSTs metastasize easily, are therapy resistant and are frequently fatal. The molecular changes underlying the malignant ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59789-4
更新日期：2020-02-19 00:00:00
abstract：:Colorectal cancer (CRC) treatment is associated with a high morbidity which may result in a reduced health-related quality of life (HRQoL). The pre-operative measurement of handgrip strength (HGS) might be a tool to predict the patient's outcome after CRC surgery. The aim of this study was to evaluate the association ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69806-1
更新日期：2020-08-03 00:00:00
abstract：:Lariatin A, an 18-residue lasso peptide encoded by the five-gene cluster larABCDE, displays potent and selective anti-mycobacterial activity. The structural feature is an N-terminal macrolactam ring, through which the C-terminal passed to form the rigid lariat-protoknot structure. In the present study, we established ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30375
更新日期：2016-07-26 00:00:00
abstract：:Transplantation of stem cell-derived insulin producing cells (IPCs) has been proposed as an alternative to islet transplantation for the treatment of diabetes mellitus. However, current IPC differentiation protocols are focused on generating functional cells from the pluripotent stem cells and tend to rely on multiste...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41823-9
更新日期：2019-03-29 00:00:00
abstract：:We have recently reported that Kupffer cells consist of two subsets, radio-resistant resident CD68+ Kupffer cells and radio-sensitive recruited CD11b+ Kupffer cells/macrophages (Mφs). Non-alcoholic steatohepatitis (NASH) is characterized not only by hepatic steatosis but also chronic inflammation and fibrosis. In the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34466
更新日期：2016-10-06 00:00:00
abstract：:Although 3,5,3'-triiodothyronine (T3) is considered to be the primary bioactive thyroid hormone (TH) due to its high affinity for TH nuclear receptors (TRs), new data suggest that 3,5-diiodothyronine (T2) can also regulate transcriptional networks. To determine the functional relevance of these bioactive THs, RNA-seq ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14913-9
更新日期：2017-11-08 00:00:00
abstract：:Processing the meaning of action language correlates with somatotopic activity in premotor cortex (PMC). A previous neurostimulation study supported a causal contribution of PMC activity to action verb understanding, but the direction of the effect was unexpected: inhibiting PMC made participants respond faster to act...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29600-6
更新日期：2018-07-30 00:00:00
abstract：:Male Wistar rats were exposed to herbicide, pendimethalin (PND) at varying oral doses of 62.5, 125 and 250 mg/kg b.w. for 14 days. Toxiological effects were assessed in terms of oxidative stress, DNA damage, histopathological alterations and induction of anti-inflammatory and apoptotic responses linked Bax, Bcl-2, IFN...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35484-3
更新日期：2018-11-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20016
更新日期：2016-01-28 00:00:00
abstract：:p53 and aldehyde dehydrogenase (ALDH) have been implicated in key tumorigenesis processes including cancer initiating cell (CIC) maintenance; however, the relationship between these two mediators remains poorly defined. In this study, ALDH isoform expression diversity was revealed in CICs with disparate p53 functional...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57758-5
更新日期：2020-01-23 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39199-x
更新日期：2019-02-21 00:00:00
abstract：:It has been a long-standing puzzle why buckled dimers of the Si(001) surface appeared symmetric below ~20 K in scanning tunneling microscopy (STM) experiments. Although such symmetric dimer images were concluded to be due to an artifact induced by STM measurements, its underlying mechanism is still veiled. Here, we de...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27868
更新日期：2016-06-13 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML version of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-24796-z
更新日期：2018-04-19 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-65652-3
更新日期：2020-05-27 00:00:00
abstract：:Cell penetrating peptides (CPPs) are peptides that can be translocated into cells and used as a carrier platform for the intracellular uptake of cargo molecules. Subject to the source of CPP sequences and their positively charged nature, the cytotoxicity and immunogenicity of conventional CPPs needs to be optimized to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11719
更新日期：2015-06-26 00:00:00
abstract：:Crystallization is the bottleneck in macromolecular crystallography; even when a protein crystallises, crystal packing often influences ligand-binding and protein-protein interaction interfaces, which are the key points of interest for functional and drug discovery studies. The human hypoxia-inducible factor prolyl hy...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76307-8
更新日期：2020-12-15 00:00:00
abstract：:Since 2001, hundreds of thousands of hours of underwater acoustic recordings have been made throughout the Southern Ocean south of 60° S. Detailed analysis of the occurrence of marine mammal sounds in these circumpolar recordings could provide novel insights into their ecology, but manual inspection of the entirety of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78995-8
更新日期：2021-01-12 00:00:00
abstract：:Pachychoroid spectrum diseases have attracted increasing attention, though their pathophysiology has yet to be fully elucidated. In this study, we assessed the vascular diameters of vortex veins in pachychoroid spectrum diseases such as central serous chorioretinopathy (CSC), pachychoroid neovasculopathy without polyp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75789-w
更新日期：2020-11-11 00:00:00
abstract：:Concurrent chemoradiation therapy (CCRT) is the treatment of choice for locally advanced non-small cell lung cancer (LA-NSCLC). Several clinical trials that combine programmed cell death 1 (PD-1) axis inhibitors with radiotherapy are in development for patients with LA-NSCLC. However, the effect of CCRT on programmed ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11949-9
更新日期：2017-09-12 00:00:00
abstract：:While previous studies separately demonstrate EEG spectral modulations during speech preparation and ERP responses to the listened speech, it is unclear whether these responses are related on a trial-by-trial basis between a speaker and listener. In order to determine whether these responses are related in real-time, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31038-9
更新日期：2018-08-27 00:00:00
abstract：:Ciliate Mesodinium species are commonly distributed in diverse aquatic systems worldwide. Among Mesodinium species, M. rubrum is closely associated with microbial food webs and red tide formation and is known to acquire chloroplasts from its cryptophyte prey for use in photosynthesis. For these reasons, Mesodinium has...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50659-2
更新日期：2019-10-01 00:00:00
abstract：:Reciprocity is frequently assumed to require complex cognitive abilities. Therefore, it has been argued that reciprocity may be restricted to animals that can meet these demands. Here, we provide evidence for the potential presence of direct reciprocity in teleost fishes. We demonstrate that in pairs of coral reef rab...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14556
更新日期：2015-09-25 00:00:00
abstract：:Demand for clinical decision support systems in medicine and self-diagnostic symptom checkers has substantially increased in recent years. Existing platforms rely on knowledge bases manually compiled through a labor-intensive process or automatically derived using simple pairwise statistics. This study explored an aut...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05778-z
更新日期：2017-07-20 00:00:00
abstract：:The auditory steady-state response (ASSR) has been used to detect auditory processing deficits in patients with psychiatric disorders. However, the methodology of ASSR recording from the brain surface has not been standardized in preclinical studies, limiting its use as a translational biomarker. The sites of maximal ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44936-3
更新日期：2019-06-11 00:00:00
abstract：:Cyanobacteria exhibit a great capacity to adapt to different environmental conditions through changes in gene expression. Although this plasticity has been extensively studied in the model cyanobacterium Synechocystis sp. PCC 6803, a detailed analysis of the coordinated transcriptional adaption across varying conditio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22168
更新日期：2016-02-29 00:00:00
