当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Late p65 nuclear translocation in glioblastoma cells indicates non-canonical TLR4 signaling and activation of DNA repair genes.

Late p65 nuclear translocation in glioblastoma cells indicates non-canonical TLR4 signaling and activation of DNA repair genes.

Abstract：

:Glioblastoma (GBM) is the most aggressive brain primary malignancy. Toll-like receptor 4 (TLR4) has a dual role in cell fate, promoting cell survival or death depending on the context. Here, we analyzed TLR4 expression in different grades of astrocytoma, and observed increased expression in tumors, mainly in GBM, compared to non-neoplastic brain tissue. TLR4 role was investigated in U87MG, a GBM mesenchymal subtype cell line, upon LPS stimulation. p65 nuclear translocation was observed in late phase, suggesting TLR4-non-canonical pathway activation. In fact, components of ripoptosome and inflammasome cascades were upregulated and they were significantly correlated in GBMs of the TCGA-RNASeq dataset. Moreover, an increased apoptotic rate was observed when the GBM-derived U87MG cells were co-treated with LPS and Temozolomide (TMZ) in comparison to TMZ alone. Increased TLR4 immunostaining was detected in nuclei of U87MG cells 12 h after LPS treatment, concomitant to activation of DNA repair genes. Time-dependent increased RAD51, FEN1 and UNG expression levels were confirmed after LPS stimulation, which may contribute to tumor cell fitness. Moreover, the combined treatment with the RAD51 inhibitor, Amuvatinib in combination with, TMZ after LPS stimulation reduced tumor cell viability more than with each treatment alone. In conclusion, our results suggest that stimulation of TLR4 combined with pharmacological inhibition of the DNA repair pathway may be an alternative treatment for GBM patients.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Moretti IF,Lerario AM,Trombetta-Lima M,Sola PR,da Silva Soares R,Oba-Shinjo SM,Marie SKN

doi

10.1038/s41598-020-79356-1

subject

Has Abstract

pub_date

2021-01-14 00:00:00

pages

1333

issue

1

issn

2045-2322

pii

10.1038/s41598-020-79356-1

journal_volume

11

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Acoustic levitation with optimized reflective metamaterials.

    abstract：:The simplest and most commonly used acoustic levitator is comprised of a transmitter and an opposing reflecting surface. This type of device, however, is only able to levitate objects along one direction, at distances multiple of half of a wavelength. In this work, we show how a customised reflective acoustic metamate...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-60978-4

    authors： Polychronopoulos S,Memoli G

    更新日期：2020-03-06 00:00:00

  • Improved risk stratification in prevention by use of a panel of selected circulating microRNAs.

    abstract：:Risk stratification is crucial in prevention. Circulating microRNAs have been proposed as biomarkers in cardiovascular disease. Here a miR panel consisting of miRs related to different cardiovascular pathophysiologies, was evaluated to predict outcome in the context of prevention. MiR-34a, miR-223, miR-378, miR-499 an...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-04040-w

    authors： Keller T,Boeckel JN,Groß S,Klotsche J,Palapies L,Leistner D,Pieper L,Stalla GK,Lehnert H,Silber S,Pittrow D,Maerz W,Dörr M,Wittchen HU,Baumeister SE,Völker U,Felix SB,Dimmeler S,Zeiher AM

    更新日期：2017-07-03 00:00:00

  • An activator of mTOR inhibits oxLDL-induced autophagy and apoptosis in vascular endothelial cells and restricts atherosclerosis in apolipoprotein E⁻/⁻ mice.

    abstract：:Oxidized low-density lipoprotein (oxLDL) inhibits mammalian target of rapamycin (mTOR) and induces autophagy and apoptosis in vascular endothelial cells (VECs) that play very critical roles for the cardiovascular homostasis. We recently defined 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one (3BDO) as a ne...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep05519

    authors： Peng N,Meng N,Wang S,Zhao F,Zhao J,Su L,Zhang S,Zhang Y,Zhao B,Miao J

    更新日期：2014-07-01 00:00:00

  • Autophagy is induced and supports virus replication in Enterovirus A71-infected human primary neuronal cells.

    abstract：:Enterovirus A71 (EV-A71), which belongs to the family Picornaviridae, can invade the central nervous system (CNS) and cause severe CNS complications or death. The EV-A71 antigen has been detected in the neurons in the brains of humans who died from EV-A71 infection. However, the effect of EV-A71 infection on human neu...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71970-3

    authors： Lin JY,Huang HI

    更新日期：2020-09-17 00:00:00

  • Marigold flower like structured Cu2NiSnS4 electrode for high energy asymmetric solid state supercapacitors.

    abstract：:The growth in energy devices and the role of supercapacitors are increasingly important in today's world. Designing an electrode material for supercapacitors using metals that have high performance, superior structure, are eco-friendly, inexpensive and highly abundant is essentially required for commercialization. In ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-75879-9

    authors： Isacfranklin M,Yuvakkumar R,Ravi G,Hong SI,Shini F,Thambidurai M,Dang C,Velauthapillai D

    更新日期：2020-11-05 00:00:00

  • Experimental proof of emergent subharmonic attenuation zones in a nonlinear locally resonant metamaterial.

    abstract：:High-performance locally resonant metamaterials represent the next frontier in materials technology due to their extraordinary properties obtained through materials design, enabling a variety of potential applications. The most exceptional feature of locally resonant metamaterials is the subwavelength size of their un...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-68894-3

    authors： Zega V,Silva PB,Geers MGD,Kouznetsova VG

    更新日期：2020-07-21 00:00:00

  • Alcohol intake and cardiovascular risk factors: A Mendelian randomisation study.

    abstract：:Mendelian randomisation studies from Asia suggest detrimental influences of alcohol on cardiovascular risk factors, but such associations are observed mainly in men. The absence of associations of genetic variants (e.g. rs671 in ALDH2) with such risk factors in women - who drank little in these populations - provides ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep18422

    authors： Cho Y,Shin SY,Won S,Relton CL,Davey Smith G,Shin MJ

    更新日期：2015-12-21 00:00:00

  • Analysis of genome-wide variants through bulked segregant RNA sequencing reveals a major gene for resistance to Plasmodiophora brassicae in Brassica oleracea.

    abstract：:Two cabbage (Brassica oleracea) cultivars 'Tekila' and 'Kilaherb' were identified as resistant to several pathotypes of Plasmodiophora brassicae. In this study, we identified a clubroot resistance gene (Rcr7) in 'Tekila' for resistance to pathotype 3 of P. brassicae from a segregating population derived from 'Tekila' ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-36187-5

    authors： Dakouri A,Zhang X,Peng G,Falk KC,Gossen BD,Strelkov SE,Yu F

    更新日期：2018-12-05 00:00:00

  • Latexin deficiency in mice up-regulates inflammation and aggravates colitis through HECTD1/Rps3/NF-κB pathway.

    abstract：:The function of Latexin (LXN) in inflammation has attracted attention. However, no data are available regarding its role in colitis. We report that LXN is a suppressor of colitis. LXN deficiency leads to the severity of colitis in DSS-induced mice, and LXN is required for the therapeutic effect of retinoic acid on col...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66789-x

    authors： Li Y,Huang B,Yang H,Kan S,Yao Y,Liu X,Pu S,He G,Khan TM,Qi G,Zhou Z,Shu W,Chen M

    更新日期：2020-06-17 00:00:00

  • Fam64a is a novel cell cycle promoter of hypoxic fetal cardiomyocytes in mice.

    abstract：:Fetal cardiomyocytes actively proliferate to form the primitive heart in utero in mammals, but they stop dividing shortly after birth. The identification of essential molecules maintaining this active cardiomyocyte proliferation is indispensable for potential adult heart regeneration. A recent study has shown that thi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-04823-1

    authors： Hashimoto K,Kodama A,Honda T,Hanashima A,Ujihara Y,Murayama T,Nishimatsu SI,Mohri S

    更新日期：2017-06-30 00:00:00

  • Author Correction: Dysregulated JAK2 expression by TrkC promotes metastasis potential, and EMT program of metastatic breast cancer.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-020-68128-6

    authors： Kim MS,Jeong J,Seo J,Kim HS,Kim SJ,Jin W

    更新日期：2020-07-06 00:00:00

  • A novel Mitosomal β-barrel Outer Membrane Protein in Entamoeba.

    abstract：:Entamoeba possesses a highly divergent mitochondrion-related organelle known as the mitosome. Here, we report the discovery of a novel protein in Entamoeba, which we name Mitosomal β-barrel Outer Membrane Protein of 30 kDa (MBOMP30). Initially identified through in silico analysis, we experimentally confirmed that MBO...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep08545

    authors： Santos HJ,Imai K,Makiuchi T,Tomii K,Horton P,Nozawa A,Ibrahim M,Tozawa Y,Nozaki T

    更新日期：2015-02-25 00:00:00

  • Stable N-doped & FeNi-decorated graphene non-precious electrocatalyst for Oxygen Reduction Reaction in Acid Medium.

    abstract：:NiFe nanoparticles-decorated & N-doped graphene is introduced as an effective and stable non-precious electrocatalyst for ORR in the acid medium. Compared to conventional Pt/C electrodes under the same conditions, the proposed nanocatalyst shows closer onset potential and current density. Typically, the observed onset...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-22114-1

    authors： Barakat NAM,El-Deen AG,Ghouri ZK,Al-Meer S

    更新日期：2018-02-28 00:00:00

  • The potential of Gantry beamline large momentum acceptance for real time tumour tracking in pencil beam scanning proton therapy.

    abstract：:Tumour tracking is an advanced radiotherapy technique for precise treatment of tumours subject to organ motion. In this work, we addressed crucial aspects of dose delivery for its realisation in pencil beam scanning proton therapy, exploring the momentum acceptance and global achromaticity of a Gantry beamline to perf...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71821-1

    authors： Fattori G,Zhang Y,Meer D,Weber DC,Lomax AJ,Safai S

    更新日期：2020-09-18 00:00:00

  • Fibroblasts play a potential role in bone destruction via osteopontin related caldesmon expression and polymerization in human non-functioning pituitary adenomas.

    abstract：:Non-functioning pituitary adenomas (NFPAs) are the most frequent pituitary tumors. The elucidation of the mechanisms of aggressive NFPAs in bone destruction is required in order to guide the clinical diagnosis and treatment of NFPAs. In the present study, we investigated the differential proteomics of fibroblasts isol...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-17679-2

    authors： Zhang LY,Ge XL,Li Z,Tang YJ,Xiong YY,Li XJ,Liu JF,Wanggou SY,Li CT,Yang K,Chen X,Hu ZL,Liu YS,Liu ZX

    更新日期：2017-12-13 00:00:00

  • NOTCH1 is critical for fibroblast-mediated induction of cardiomyocyte specialization into ventricular conduction system-like cells in vitro.

    abstract：:Cardiac fibroblasts are present throughout the myocardium and are enriched in the microenvironment surrounding the ventricular conduction system (VCS). Several forms of arrhythmias are linked to VCS abnormalities, but it is still unclear whether VCS malformations are cardiomyocyte autonomous or could be linked to cros...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-73159-0

    authors： Ribeiro da Silva A,Neri EA,Turaça LT,Dariolli R,Fonseca-Alaniz MH,Santos-Miranda A,Roman-Campos D,Venturini G,Krieger JE

    更新日期：2020-09-30 00:00:00

  • Predictive computational modeling to define effective treatment strategies for bone metastatic prostate cancer.

    abstract：:The ability to rapidly assess the efficacy of therapeutic strategies for incurable bone metastatic prostate cancer is an urgent need. Pre-clinical in vivo models are limited in their ability to define the temporal effects of therapies on simultaneous multicellular interactions in the cancer-bone microenvironment. Inte...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep29384

    authors： Cook LM,Araujo A,Pow-Sang JM,Budzevich MM,Basanta D,Lynch CC

    更新日期：2016-07-14 00:00:00

  • Global identification and analysis of isozyme-specific possible substrates crosslinked by transglutaminases using substrate peptides in mouse liver fibrosis.

    abstract：:The transglutaminase (TG) family comprises eight isozymes that form the isopeptide bonds between glutamine and lysine residues and contribute to the fibrotic diseases via crosslinking-mediated stabilization of ECM and the activation of TGF-β in several tissues. However, despite a growing body of evidence implicating T...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep45049

    authors： Tatsukawa H,Tani Y,Otsu R,Nakagawa H,Hitomi K

    更新日期：2017-03-22 00:00:00

  • Association between sea lice (Lepeophtheirus salmonis) infestation on Atlantic salmon farms and wild Pacific salmon in Muchalat Inlet, Canada.

    abstract：:Growth in salmon aquaculture over the past two decades has raised concerns regarding the potential impacts of the industry on neighboring ecosystems and wild fish productivity. Despite limited evidence, sea lice have been identified as a major cause for the decline in some wild Pacific salmon populations on the west c...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-22458-8

    authors： Nekouei O,Vanderstichel R,Thakur K,Arriagada G,Patanasatienkul T,Whittaker P,Milligan B,Stewardson L,Revie CW

    更新日期：2018-03-05 00:00:00

  • Chaotic optical power dropouts driven by low frequency bias forcing in a mid-infrared quantum cascade laser.

    abstract：:Mid-infrared quantum cascade lasers operating under external optical feedback and external periodic bias forcing are shown to exhibit a deterministic chaotic pattern composed of frequencies which are linked to the one of the forcing. Results also show that both the amplitude and the frequency of the forcing play a key...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-40861-7

    authors： Spitz O,Wu J,Carras M,Wong CW,Grillot F

    更新日期：2019-03-14 00:00:00

  • Genetic variation across RNA metabolism and cell death gene networks is implicated in the semantic variant of primary progressive aphasia.

    abstract：:The semantic variant of primary progressive aphasia (svPPA) is a clinical syndrome characterized by neurodegeneration and progressive loss of semantic knowledge. Unlike many other forms of frontotemporal lobar degeneration (FTLD), svPPA has a highly consistent underlying pathology composed of TDP-43 (a regulator of RN...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-46415-1

    authors： Bonham LW,Steele NZR,Karch CM,Broce I,Geier EG,Wen NL,Momeni P,Hardy J,Miller ZA,Gorno-Tempini ML,Hess CP,Lewis P,Miller BL,Seeley WW,Manzoni C,Desikan RS,Baranzini SE,Ferrari R,Yokoyama JS,International FTD-Genomic

    更新日期：2019-07-26 00:00:00

  • A framework to identify structured behavioral patterns within rodent spatial trajectories.

    abstract：:Animal behavior is highly structured. Yet, structured behavioral patterns-or "statistical ethograms"-are not immediately apparent from the full spatiotemporal data that behavioral scientists usually collect. Here, we introduce a framework to quantitatively characterize rodent behavior during spatial (e.g., maze) navig...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-79744-7

    authors： Donnarumma F,Prevete R,Maisto D,Fuscone S,Irvine EM,van der Meer MAA,Kemere C,Pezzulo G

    更新日期：2021-01-11 00:00:00

  • PD-L1 predicts poor prognosis for nasopharyngeal carcinoma irrespective of PD-1 and EBV-DNA load.

    abstract：:Programmed death-1 (PD-1) is an immunosuppressive receptor functionally bound with programmed death-ligand 1 (PD-L1), which has been reported in various malignancies. However, only a few studies are available for the clinical significance of PD-1/PD-L1 in nasopharyngeal carcinoma (NPC). In this study, we aim to invest...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep43627

    authors： Zhou Y,Shi D,Miao J,Wu H,Chen J,Zhou X,Hu D,Zhao C,Deng W,Xie C

    更新日期：2017-03-03 00:00:00

  • High-throughput and automated diagnosis of antimicrobial resistance using a cost-effective cellphone-based micro-plate reader.

    abstract：:Routine antimicrobial susceptibility testing (AST) can prevent deaths due to bacteria and reduce the spread of multi-drug-resistance, but cannot be regularly performed in resource-limited-settings due to technological challenges, high-costs, and lack of trained professionals. We demonstrate an automated and cost-effec...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep39203

    authors： Feng S,Tseng D,Di Carlo D,Garner OB,Ozcan A

    更新日期：2016-12-15 00:00:00

  • Deep placement of controlled-release urea effectively enhanced nitrogen use efficiency and fresh ear yield of sweet corn in fluvo-aquic soil.

    abstract：:Application of controlled-release urea (CRU) improves crop yield and nitrogen use efficiency (NUE) compared with conventional urea. However, the effectiveness of CRU differs with fertilization placement. A two site-year field experiment was carried out in fluvo-aquic soil in central China to study the effects of two N...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56912-y

    authors： Liu W,Xiong Y,Xu X,Xu F,Hussain S,Xiong H,Yuan J

    更新日期：2019-12-30 00:00:00

  • DaReUS-Loop: accurate loop modeling using fragments from remote or unrelated proteins.

    abstract：:Despite efforts during the past decades, loop modeling remains a difficult part of protein structure modeling. Several approaches have been developed in the framework of crystal structures. However, for homology models, the modeling of loops is still far from being solved. We propose DaReUS-Loop, a data-based approach...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-32079-w

    authors： Karami Y,Guyon F,De Vries S,Tufféry P

    更新日期：2018-09-12 00:00:00

  • Mdm2 enhances ligase activity of parkin and facilitates mitophagy.

    abstract：:Loss-of-function mutations in the E3 ubiquitin ligase parkin have been implicated in the death of dopaminergic neurons in the substantia nigra, which is the root cause of dopamine deficit in the striatum in Parkinson's disease. Parkin ubiquitinates proteins on mitochondria that lost membrane potential, promoting the e...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-61796-4

    authors： Kook S,Zhan X,Thibeault K,Ahmed MR,Gurevich VV,Gurevich EV

    更新日期：2020-03-19 00:00:00

  • First-In-Human Phase 1 Clinical Trials - A Single-Center Experience In The Era Of Modern Oncotherapeutics.

    abstract：:In the era of precision medicine the treatment options for cancer patients and subsequent outcomes are expected to improve. We present a review of patients enrolled in first-in-human Phase1 trials at University of Alabama at Birmingham. Between 1/2015-6/2017, 162 cancer patients (whole cohort, WC) were enrolled on pha...

    journal_title：Scientific reports

    pub_type： 杂志文章,meta分析,评审

    doi：10.1038/s41598-020-64906-4

    authors： Paluri RK,Li P,Anderson A,Nandagopal L,McArdle T,Young M,Robert F,Naik G,Saleh M

    更新日期：2020-05-13 00:00:00

  • Solving the controversy of healthier organic fruit: Leaf wounding triggers distant gene expression response of polyphenol biosynthesis in strawberry fruit (Fragaria x ananassa).

    abstract：:The claim that organic agriculture produces higher levels of phytochemicals has been controversial for decades. Using strawberries as a model crop in field conditions, a preharvest leaf wounding stress was applied to study the production of phytochemicals in fruits. As a result phenolic compounds (PCs) and total solub...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-55033-w

    authors： Ibanez F,Bang WY,Lombardini L,Cisneros-Zevallos L

    更新日期：2019-12-17 00:00:00

  • Fish oil intake induces UCP1 upregulation in brown and white adipose tissue via the sympathetic nervous system.

    abstract：:Brown adipose tissue (BAT) plays a central role in regulating energy homeostasis, and may provide novel strategies for the treatment of human obesity. BAT-mediated thermogenesis is regulated by mitochondrial uncoupling protein 1 (UCP1) in classical brown and ectopic beige adipocytes, and is controlled by sympathetic n...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep18013

    authors： Kim M,Goto T,Yu R,Uchida K,Tominaga M,Kano Y,Takahashi N,Kawada T

    更新日期：2015-12-17 00:00:00