Transcriptomic Analysis of Aedes aegypti in Response to Mosquitocidal Bacillus thuringiensis LLP29 Toxin.

Abstract:

:Globally, Aedes aegypti is one of the most dangerous mosquitoes that plays a crucial role as a vector for human diseases, such as yellow fever, dengue, and chikungunya. To identify (1) transcriptomic basis of midgut (2) key genes that are involved in the toxicity process by a comparative transcriptomic analysis between the control and Bacillus thuringiensis (Bt) toxin (LLP29 proteins)-treated groups. Next-generation sequencing technology was used to sequence the midgut transcriptome of A. aegypti. A total of 17130 unigenes, including 574 new unigenes, were identified containing 16358 (95.49%) unigenes that were functionally annotated. According to differentially expressed gene (DEG) analysis, 557 DEGs were annotated, including 226 upregulated and 231 downregulated unigenes in the Bt toxin-treated group. A total of 442 DEGs were functionally annotated; among these, 33 were specific to multidrug resistance, 6 were immune-system-related (Lectin, Defensin, Lysozyme), 28 were related to putative proteases, 7 were lipase-related, 8 were related to phosphatases, and 30 were related to other transporters. In addition, the relative expression of 28 DEGs was further confirmed through quantitative real time polymerase chain reaction. The results provide a transcriptomic basis for the identification and functional authentication of DEGs in A. aegypti.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Batool K,Alam I,Wu S,Liu W,Zhao G,Chen M,Wang J,Xu J,Huang T,Pan X,Yu X,Guan X,Xu L,Zhang L

doi

10.1038/s41598-018-30741-x

subject

Has Abstract

pub_date

2018-08-23 00:00:00

pages

12650

issue

1

issn

2045-2322

pii

10.1038/s41598-018-30741-x

journal_volume

8

pub_type

杂志文章
  • Helicobacter pylori colonization critically depends on postprandial gastric conditions.

    abstract::The risk of Helicobacter pylori infection is highest in childhood, but the colonization process of the stomach mucosa is poorly understood. We used anesthetized Mongolian gerbils to study the initial stages of H. pylori colonization. Prandial and postprandial gastric conditions characteristic of humans of different ag...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep00994

    authors: Bücker R,Azevedo-Vethacke M,Groll C,Garten D,Josenhans C,Suerbaum S,Schreiber S

    更新日期:2012-01-01 00:00:00

  • Same habitat types but different use: evidence of context-dependent habitat selection in roe deer across populations.

    abstract::With the surge of GPS-technology, many studies uncovered space use of mobile animals and shed light on the underlying behavioral mechanisms of habitat selection. Habitat selection and variation in either occurrence or strength of functional responses (i.e. how selection changes with availability) have given new insigh...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-23111-0

    authors: William G,Jean-Michel G,Sonia S,Christophe B,Atle M,Nicolas M,Maryline P,Clément C

    更新日期:2018-03-23 00:00:00

  • Climate change drives trait-shifts in coral reef communities.

    abstract::Climate change is expected to have profound, partly unforeseeable effects on the composition of functional traits of complex ecosystems, such as coral reefs, and some ecosystem properties are at risk of disappearing. This study applies a novel spatially explicit, individual-based model to explore three critical life h...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-38962-4

    authors: Kubicek A,Breckling B,Hoegh-Guldberg O,Reuter H

    更新日期:2019-03-06 00:00:00

  • Periostin as a novel biomarker for postoperative recurrence of chronic rhinosinitis with nasal polyps.

    abstract::We previously reported that chronic rhinosinusitis with nasal polyps (CRSwNP) was subdivided into four chronic rhinosinusitis (CRS) subtypes using the JESREC scoring system. We sought to identify the gene expression profile and biomarkers related with CRSwNP by RNA-sequence. RNA-sequencing was performed to identify di...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-29612-2

    authors: Ninomiya T,Noguchi E,Haruna T,Hasegawa M,Yoshida T,Yamashita Y,Okano M,Yoshida N,Haruna S,Sakuma Y,Ohta S,Ono J,Izuhara K,Okada M,Kidoguchi M,Tokunaga T,Okamoto M,Kanno M,Sakashita M,Takabayashi T,Narita N,Fujie

    更新日期:2018-07-30 00:00:00

  • Synthetic surfactant- and cross-linker-free preparation of highly stable lipid-polymer hybrid nanoparticles as potential oral delivery vehicles.

    abstract::The toxicity associated with concentrated synthetic surfactants and the poor stability at gastrointestinal condition are two major constraints for practical applications of solid lipid nanoparticles (SLN) as oral delivery vehicles. In this study, a synthetic surfactant-free and cross-linker-free method was developed t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-02867-x

    authors: Wang T,Xue J,Hu Q,Zhou M,Chang C,Luo Y

    更新日期:2017-06-05 00:00:00

  • Exploratory Data Analysis of Adverse Birth Outcomes and Exposure to Oxides of Nitrogen Using Interactive Parallel Coordinates Plot Technique.

    abstract::We propose that a parallel coordinates plot can be used to study multidimensional data particularly to explore discovery of patterns across the variables. This can assist researchers from the health sciences to visualize their cohort data with interactive data analysis. The study used data from Mother and Child in the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-64471-w

    authors: Mitku AA,Zewotir T,North D,Naidoo RN

    更新日期:2020-04-30 00:00:00

  • An improved overlap extension PCR for simultaneous multiple sites large fragments insertion, deletion and substitution.

    abstract::The existing molecular cloning methods are often limited by the availability of suitable restriction sites. It is still a challenge for simultaneous cloning of multiple fragments into different sites of a single vector. Here we developed a novel method named improved overlap extension PCR (IOEP) for restriction enzyme...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-52122-8

    authors: Guo W,Xie B,Jiang M,Zhu XJ,Qiu M,Dai ZM

    更新日期:2019-10-30 00:00:00

  • Identification of key metabolic changes in renal interstitial fibrosis rats using metabonomics and pharmacology.

    abstract::Renal fibrosis is one of the important pathways involved in end-stage renal failure. Investigating the metabolic changes in the progression of disease may enhance the understanding of its pathogenesis and therapeutic information. In this study, (1)H-nuclear magnetic resonance (NMR)-based metabonomics was firstly used ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27194

    authors: Zhao L,Dong M,Liao S,Du Y,Zhou Q,Zheng H,Chen M,Ji J,Gao H

    更新日期:2016-06-03 00:00:00

  • Qubit lattice coherence induced by electromagnetic pulses in superconducting metamaterials.

    abstract::Quantum bits (qubits) are at the heart of quantum information processing schemes. Currently, solid-state qubits, and in particular the superconducting ones, seem to satisfy the requirements for being the building blocks of viable quantum computers, since they exhibit relatively long coherence times, extremely low diss...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29374

    authors: Ivić Z,Lazarides N,Tsironis GP

    更新日期:2016-07-12 00:00:00

  • IRE1α-XBP1 is a novel branch in the transcriptional regulation of Ucp1 in brown adipocytes.

    abstract::The unfolded protein response (UPR) not only resolves endoplasmic reticulum (ER) stress, but also regulates cellular physiological functions. In this study, we first linked the UPR to the physiological roles of brown adipose tissue (BAT). BAT is one of the tissues that control energy homeostasis in the body. Brown adi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep16580

    authors: Asada R,Kanemoto S,Matsuhisa K,Hino K,Cui M,Cui X,Kaneko M,Imaizumi K

    更新日期:2015-11-16 00:00:00

  • Theoretical investigation of heterogeneous wettability in porous media using NMR.

    abstract::It is highly important to understand the heterogeneous wettability properties of porous media for enhanced oil recovery (EOR). However, wettability measurements are still challenging in directly investigating the wettability of porous media. In this paper, we propose a multidimensional nuclear magnetic resonance (NMR)...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-31803-w

    authors: Wang J,Xiao L,Liao G,Zhang Y,Guo L,Arns CH,Sun Z

    更新日期:2018-09-07 00:00:00

  • Stress affects the epigenetic marks added by natural transposable element insertions in Drosophila melanogaster.

    abstract::Transposable elements are emerging as an important source of cis-acting regulatory sequences and epigenetic marks that could influence gene expression. However, few studies have dissected the role of specific transposable element insertions on epigenetic gene regulation. Bari-Jheh is a natural transposon that mediates...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-30491-w

    authors: Guio L,Vieira C,González J

    更新日期:2018-08-15 00:00:00

  • A transcriptome atlas of rabbit revealed by PacBio single-molecule long-read sequencing.

    abstract::It is widely acknowledged that transcriptional diversity largely contributes to biological regulation in eukaryotes. Since the advent of second-generation sequencing technologies, a large number of RNA sequencing studies have considerably improved our understanding of transcriptome complexity. However, it still remain...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-08138-z

    authors: Chen SY,Deng F,Jia X,Li C,Lai SJ

    更新日期:2017-08-09 00:00:00

  • Surface enhanced Raman spectroscopy of Chlamydia trachomatis and Neisseria gonorrhoeae for diagnostics, and extra-cellular metabolomics and biochemical monitoring.

    abstract::SERS spectra excited at 785 nm of the bacteria Chlamydia trahomatis (elementary bodies, EB) and Neisseria gonorrheoae, the causative pathogens for the two most common sexually transmitted diseases (STD), chlamydia and gonorrhea, respectively, are reported. Although both are Gram-negative bacteria, the SERS signatures ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-23562-5

    authors: Chen Y,Premasiri WR,Ziegler LD

    更新日期:2018-03-26 00:00:00

  • Cumulative Resting Heart Rate Exposure and Risk of All-Cause Mortality: Results from the Kailuan Cohort Study.

    abstract::The relationship between cumulative exposure to resting heart rate (cumRHR) and mortality remain unclear in the general population. In the Kailuan cohort study, resting heart rate (RHR) was repeatedly measured at baseline and at years 2 and 4 by electrocardiogram among 47,311 adults aged 48.70 ± 11.68. The cumRHR was ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40212

    authors: Zhao Q,Li H,Wang A,Guo J,Yu J,Luo Y,Chen S,Tao L,Li Y,Li A,Guo X,Wu S

    更新日期:2017-01-09 00:00:00

  • Genome-wide analysis of the aquaporin genes in melon (Cucumis melo L.).

    abstract::Melon (Cucumis melo L.) is a very important crop throughout the world and has great economic importance, in part due to its nutritional properties. It prefers well-drained soil with low acidity and has a strong demand for water during fruit set. Therefore, a correct water balance-involving aquaporins-is necessary to m...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-79250-w

    authors: Lopez-Zaplana A,Nicolas-Espinosa J,Carvajal M,Bárzana G

    更新日期:2020-12-17 00:00:00

  • Development of a rapid and economic in vivo electrocardiogram platform for cardiovascular drug assay and electrophysiology research in adult zebrafish.

    abstract::Zebrafish is a popular and favorable model organism for cardiovascular research, with an increasing number of studies implementing functional assays in the adult stage. For example, the application of electrocardiography (ECG) in adult zebrafish has emerged as an important tool for cardiac pathophysiology, toxicity, a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-33577-7

    authors: Lin MH,Chou HC,Chen YF,Liu W,Lee CC,Liu LY,Chuang YJ

    更新日期:2018-10-30 00:00:00

  • Bioaggregate of photo-fermentative bacteria for enhancing continuous hydrogen production in a sequencing batch photobioreactor.

    abstract::Hydrogen recovery through solar-driven biomass conversion by photo-fermentative bacteria (PFB) has been regarded as a promising way for sustainable energy production. However, a considerable fraction of organic substrate was consumed for the growth of PFB as biocatalysts, furthermore, these PFB were continuously washe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep16174

    authors: Xie GJ,Liu BF,Wang RQ,Ding J,Ren HY,Zhou X,Ren NQ

    更新日期:2015-11-05 00:00:00

  • Tension-type headache in the Emergency Department Diagnosis and misdiagnosis: The TEDDi study.

    abstract::Headache is a common reason to visit the emergency department (ED). Tension-type headache (TTH) is the commonest headache. The diagnosis of TTH implies a mild condition, with no need for special tests. We evaluated the use of the International Classification of Headache Disorders (ICHD) criteria for TTH in the ED. We ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59171-4

    authors: García-Azorín D,Farid-Zahran M,Gutiérrez-Sánchez M,González-García MN,Guerrero AL,Porta-Etessam J

    更新日期:2020-02-12 00:00:00

  • Serum albumin cysteine trioxidation is a potential oxidative stress biomarker of type 2 diabetes mellitus.

    abstract::Metabolic disorders in T2DM generate multiple sources of free radicals and oxidative stress that accelerate nonenzymatic degenerative protein modifications (DPMs) such as protein oxidation, disrupt redox signaling and physiological function, and remain a major risk factor for clinical diabetic vascular complications. ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-62341-z

    authors: Paramasivan S,Adav SS,Ngan SC,Dalan R,Leow MK,Ho HH,Sze SK

    更新日期:2020-04-15 00:00:00

  • Structural and physical determinants of the proboscis-sucking pump complex in the evolution of fluid-feeding insects.

    abstract::Fluid-feeding insects have evolved a unique strategy to distribute the labor between a liquid-acquisition device (proboscis) and a sucking pump. We theoretically examined physical constraints associated with coupling of the proboscis and sucking pump into a united functional organ. Classification of fluid feeders with...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06391-w

    authors: Kornev KG,Salamatin AA,Adler PH,Beard CE

    更新日期:2017-07-26 00:00:00

  • Alternative splicing complexity contributes to genetic improvement of drought resistance in the rice maintainer HuHan2B.

    abstract::Water-saving and drought-resistantce rice (WDR) breeding practices have greatly increased grain yield and drought resistance. To study the genetic basis of adaptation to drought, transcriptome sequences from the WDR maintainer line HuHan2B and the recurrent parent HanFengB were analyzed for alternative splicing (AS) c...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-12020-3

    authors: Wei H,Lou Q,Xu K,Yan M,Xia H,Ma X,Yu X,Luo L

    更新日期:2017-09-15 00:00:00

  • Interferon γ-induced GTPase promotes invasion of Listeria monocytogenes into trophoblast giant cells.

    abstract::Listeria monocytogenes is well known for having the ability to cross the placental barrier, leading to fetal infections and abortion. However, the mechanisms leading to infectious abortion are poorly understood. In this study, we demonstrate that interferon γ-induced GTPase (IGTP) contributes to the invasion of L. mon...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08195

    authors: Tachibana M,Hashino M,Watanabe K,Shimizu T,Watarai M

    更新日期:2015-02-03 00:00:00

  • Lattice disorder effect on magnetic ordering of iron arsenides.

    abstract::This study investigates magnetic ordering temperature in nano- and mesoscale structural features in an iron arsenide. Although magnetic ground states in quantum materials can be theoretically predicted from known crystal structures and chemical compositions, the ordering temperature is harder to pinpoint due to potent...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-56301-5

    authors: Sefat AS,Wang XP,Liu Y,Zou Q,Fu M,Gai Z,Ganesan K,Vohra Y,Li L,Parker DS

    更新日期:2019-12-27 00:00:00

  • An archaeal RNA binding protein, FAU-1, is a novel ribonuclease related to rRNA stability in Pyrococcus and Thermococcus.

    abstract::Ribosome biogenesis and turnover are processes necessary for cell viability and proliferation, and many kinds of proteins are known to regulate these processes. However, many questions still remain, especially in the Archaea. Generally, several ribonucleases are required to process precursor rRNAs to their mature form...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-13062-3

    authors: Ikeda Y,Okada Y,Sato A,Kanai T,Tomita M,Atomi H,Kanai A

    更新日期:2017-10-04 00:00:00

  • A novel approach for discovering stochastic models behind data applied to El Niño-Southern Oscillation.

    abstract::Stochastic differential equations (SDEs) are ubiquitous across disciplines, and uncovering SDEs driving observed time series data is a key scientific challenge. Most previous work on this topic has relied on restrictive assumptions, undermining the generality of these approaches. We present a novel technique to uncove...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-021-81162-2

    authors: Olson R,An SI,Kim SK,Fan Y

    更新日期:2021-01-29 00:00:00

  • miR-200c-driven Mesenchymal-To-Epithelial Transition is a Therapeutic Target in Uterine Carcinosarcomas.

    abstract::Uterine carcinosarcomas (UCSs) are highly aggressive malignancies associated with poor prognoses and limited treatment options. These tumors are hypothesized to develop from the endometrial adenocarcinoma (EAC) through epithelial-mesenchymal transition (EMT). We test this long-standing hypothesis by depleting miR-200,...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-03972-7

    authors: Tseng JH,Bisogna M,Hoang LN,Olvera N,Rodriguez-Aguayo C,Lopez-Berestein G,Sood AK,Levine DA,Jelinic P

    更新日期:2017-06-15 00:00:00

  • On detection of periodicity in C-reactive protein (CRP) levels.

    abstract::C-reactive protein (CRP) is an acute-phase plasma protein that can be used as a biomarker for activation of the immune system. A spectral analysis of CRP level over time for patients with gynaecological tumours has been reported by Madondo et al., using a periodogram method, suggesting that there is no significant per...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-30469-8

    authors: Dorraki M,Fouladzadeh A,Salamon SJ,Allison A,Coventry BJ,Abbott D

    更新日期:2018-08-10 00:00:00

  • Differences in lung cancer characteristics and mortality rate between screened and non-screened cohorts.

    abstract::Screening programs for lung cancer aim to allow diagnosis at the early stage, and therefore the decline in mortality rates. Thus, the aim of this retrospective cohort study was to the comparison of screened and non-screened lung cancer in terms of lung cancer characteristics, overdiagnosis and survival rate. A retrosp...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-56025-6

    authors: Wu FZ,Kuo PL,Huang YL,Tang EK,Chen CS,Wu MT,Lin YP

    更新日期:2019-12-18 00:00:00

  • The full annual carbon balance of a subtropical coniferous plantation is highly sensitive to autumn precipitation.

    abstract::Deep understanding of the effects of precipitation on carbon budgets is essential to assess the carbon balance accurately and can help predict potential variation within the global change context. Therefore, we addressed this issue by analyzing twelve years (2003-2014) of observations of carbon fluxes and their corres...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10485-w

    authors: Xu M,Wang H,Wen X,Zhang T,Di Y,Wang Y,Wang J,Cheng C,Zhang W

    更新日期:2017-08-30 00:00:00