Abstract：
:The existing molecular cloning methods are often limited by the availability of suitable restriction sites. It is still a challenge for simultaneous cloning of multiple fragments into different sites of a single vector. Here we developed a novel method named improved overlap extension PCR (IOEP) for restriction enzyme independent cloning of large fragments. The addition of primers enables IOEP to exponentially amplify the overlap extension product, thus greatly improves the amplification efficiency of large fragments. Moreover, coupled with the benefit of T4 DNA polymerase to improve cloning efficiency, our method can be used to simultaneously insert, delete and replace multiple DNA fragments at different sites.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Guo W,Xie B,Jiang M,Zhu XJ,Qiu M,Dai ZMdoi
10.1038/s41598-019-52122-8subject
Has Abstractpub_date
2019-10-30 00:00:00pages
15637issue
1issn
2045-2322pii
10.1038/s41598-019-52122-8journal_volume
9pub_type
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abstract：:The aesthetic value of landscapes contributes to human well-being. However, studies which have investigated the link between biodiversity and ecosystem services have not taken aesthetic value into account. In this study we evaluated how the aesthetics of coralligenous reefs, a key marine ecosystem in the Mediterranean...
journal_title：Scientific reports
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 已发布勘误
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journal_title：Scientific reports
pub_type： 临床试验,杂志文章
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pub_type： 杂志文章,meta分析
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