Abstract:
:Hydrogen peroxide (H2O2) is a major reactive oxygen species in unicellular and multicellular organisms, and is produced extracellularly in response to external stresses and internal cues1-4. H2O2 enters cells through aquaporin membrane proteins and covalently modifies cytoplasmic proteins to regulate signalling and cellular processes. However, whether sensors for H2O2 also exist on the cell surface remains unknown. In plant cells, H2O2 triggers an influx of Ca2+ ions, which is thought to be involved in H2O2 sensing and signalling. Here, by using forward genetic screens based on Ca2+ imaging, we isolated hydrogen-peroxide-induced Ca2+ increases (hpca) mutants in Arabidopsis, and identified HPCA1 as a leucine-rich-repeat receptor kinase belonging to a previously uncharacterized subfamily that features two extra pairs of cysteine residues in the extracellular domain. HPCA1 is localized to the plasma membrane and is activated by H2O2 via covalent modification of extracellular cysteine residues, which leads to autophosphorylation of HPCA1. HPCA1 mediates H2O2-induced activation of Ca2+ channels in guard cells and is required for stomatal closure. Our findings help to identify how the perception of extracellular H2O2 is integrated with responses to various external stresses and internal cues in plants, and have implications for the design of crops with enhanced fitness.
journal_name
Naturejournal_title
Natureauthors
Wu F,Chi Y,Jiang Z,Xu Y,Xie L,Huang F,Wan D,Ni J,Yuan F,Wu X,Zhang Y,Wang L,Ye R,Byeon B,Wang W,Zhang S,Sima M,Chen S,Zhu M,Pei J,Johnson DM,Zhu S,Cao X,Pei C,Zai Z,Liu Y,Liu T,Swift GB,Zhang W,Ydoi
10.1038/s41586-020-2032-3subject
Has Abstractpub_date
2020-02-01 00:00:00pages
577-581issue
7796eissn
0028-0836issn
1476-4687pii
10.1038/s41586-020-2032-3journal_volume
578pub_type
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