Abstract:
:Two large-scale yeast two-hybrid screens were undertaken to identify protein-protein interactions between full-length open reading frames predicted from the Saccharomyces cerevisiae genome sequence. In one approach, we constructed a protein array of about 6,000 yeast transformants, with each transformant expressing one of the open reading frames as a fusion to an activation domain. This array was screened by a simple and automated procedure for 192 yeast proteins, with positive responses identified by their positions in the array. In a second approach, we pooled cells expressing one of about 6,000 activation domain fusions to generate a library. We used a high-throughput screening procedure to screen nearly all of the 6,000 predicted yeast proteins, expressed as Gal4 DNA-binding domain fusion proteins, against the library, and characterized positives by sequence analysis. These approaches resulted in the detection of 957 putative interactions involving 1,004 S. cerevisiae proteins. These data reveal interactions that place functionally unclassified proteins in a biological context, interactions between proteins involved in the same biological function, and interactions that link biological functions together into larger cellular processes. The results of these screens are shown here.
journal_name
Naturejournal_title
Natureauthors
Uetz P,Giot L,Cagney G,Mansfield TA,Judson RS,Knight JR,Lockshon D,Narayan V,Srinivasan M,Pochart P,Qureshi-Emili A,Li Y,Godwin B,Conover D,Kalbfleisch T,Vijayadamodar G,Yang M,Johnston M,Fields S,Rothberg JMdoi
10.1038/35001009keywords:
subject
Has Abstractpub_date
2000-02-10 00:00:00pages
623-7issue
6770eissn
0028-0836issn
1476-4687journal_volume
403pub_type
杂志文章相关文献
NATURE文献大全abstract::The ice-rich surface of the jovian satellite Europa is sparsely cratered, suggesting that this moon might be geologically active today. Moreover, models of the satellite's interior indicate that tidal interactions with Jupiter might produce enough heat to maintain a subsurface liquid water layer. But the mechanisms of...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/34862
更新日期:1998-01-22 00:00:00
abstract::Laboratory measurements of sound velocities of high-pressure minerals provide crucial information on the composition and constitution of the deep mantle via comparisons with observed seismic velocities. Calcium silicate (CaSiO3) perovskite (CaPv) is a high-pressure phase that occurs at depths greater than about 560 ki...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-018-0816-5
更新日期:2019-01-01 00:00:00
abstract::Conrad et al. have generated human adult germline stem cells (haGSCs) from human testicular tissue, which they claim have similar pluripotent properties to human embryonic stem cells (hESCs). Here we investigate the pluripotency of haGSCs by using global gene-expression analysis based on their gene array data and comp...
journal_title:Nature
pub_type: 评论,杂志文章
doi:10.1038/nature09089
更新日期:2010-06-24 00:00:00
abstract::Pancreatic ductal adenocarcinoma (PDAC) remains recalcitrant to all forms of cancer treatment and carries a five-year survival rate of only 8%1. Inhibition of oncogenic KRAS (hereafter KRAS*), the earliest lesion in disease development that is present in more than 90% of PDACs, and its signalling surrogates has yielde...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1062-1
更新日期:2019-04-01 00:00:00
abstract::An Amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41586-019-1890-z
更新日期:2020-01-01 00:00:00
abstract::The circadian clock imposes daily rhythms in cell proliferation, metabolism, inflammation and DNA damage response. Perturbations of these processes are hallmarks of cancer and chronic circadian rhythm disruption predisposes individuals to tumour development. This raises the hypothesis that pharmacological modulation o...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature25170
更新日期:2018-01-18 00:00:00
abstract::Excitation-contraction coupling in skeletal muscle involves a voltage sensor in the plasma membrane which, in response to depolarization, causes an intracellular calcium-release channel to open. The skeletal isoform of the ryanodine receptor (RyR-1) functions as the Ca2+-release channel and the dihydropyridine recepto...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/380072a0
更新日期:1996-03-07 00:00:00
abstract::Bacterial adhesion pili are designed to bind specifically and maintain attachment of bacteria to target cells. Uropathogenic P-pili are sufficiently mechanically resilient to resist the cleansing action of urine flow that removes most other bacteria. P-pili are 68 A in diameter and approximately 1 micron long, and are...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/373164a0
更新日期:1995-01-12 00:00:00
abstract::Here we describe the LifeTime Initiative, which aims to track, understand and target human cells during the onset and progression of complex diseases, and to analyse their response to therapy at single-cell resolution. This mission will be implemented through the development, integration and application of single-cell...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/s41586-020-2715-9
更新日期:2020-11-01 00:00:00
abstract::Animal microRNAs (miRNAs) regulate gene expression by inhibiting translation and/or by inducing degradation of target messenger RNAs. It is unknown how much translational control is exerted by miRNAs on a genome-wide scale. We used a new proteomic approach to measure changes in synthesis of several thousand proteins i...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature07228
更新日期:2008-09-04 00:00:00
abstract::Division of spermatogonial stem cells produces daughter cells that either maintain their stem cell identity or undergo differentiation to form mature sperm. The Sertoli cell, the only somatic cell within seminiferous tubules, provides the stem cell niche through physical support and expression of surface proteins and ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03894
更新日期:2005-08-18 00:00:00
abstract::Transmission of obligate bacterial symbionts between generations is vital for the survival of the host. Although the larvae of certain hydrothermal vent tubeworms (Vestimentifera, Siboglinidae) are symbiont-free and possess a transient digestive system, these structures are lost during development, resulting in adult ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04793
更新日期:2006-05-18 00:00:00
abstract::Improvement in the clinical outcome of lung cancer is likely to be achieved by identification of the molecular events that underlie its pathogenesis. Here we show that a small inversion within chromosome 2p results in the formation of a fusion gene comprising portions of the echinoderm microtubule-associated protein-l...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature05945
更新日期:2007-08-02 00:00:00
abstract::Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-consuming process. I...
journal_title:Nature
pub_type: 杂志文章
doi:
更新日期:1997-05-29 00:00:00
abstract::Some Pacific island societies, such as those of Easter Island and Mangareva, inadvertently contributed to their own collapse by causing massive deforestation. Others retained forest cover and survived. How can those fateful differences be explained? Although the answers undoubtedly involve both different cultural resp...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02801
更新日期:2004-09-23 00:00:00
abstract::The primary structure of the poliovirus genome has been determined. The RNA molecule is 7,433 nucleotides long, polyadenylated at the 3' terminus, and covalently linked to a small protein (VPg) at the 5' terminus. An open reading frame of 2,207 consecutive triplets spans over 89% of the nucleotide sequence and codes f...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/291547a0
更新日期:1981-06-18 00:00:00
abstract::'Identifier' or ID sequences are present in 62% of the RNA polymerase II and III transcripts made in vitro from brain nuclei but in fewer than 4% of the transcripts made from the nuclei of other tissues. An homologous 160-nucleotide cytoplasmic poly(A)+ RNA species, BC1, and a smaller species, BC2, are located in vivo...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/308237a0
更新日期:1984-03-15 00:00:00
abstract::The exceptionally broad species diversity of vascular plant genera in east Asian temperate forests, compared with their sister taxa in North America, has been attributed to the greater climatic diversity of east Asia, combined with opportunities for allopatric speciation afforded by repeated fragmentation and coalesce...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35093166
更新日期:2001-09-13 00:00:00
abstract::The guanine nucleotide-binding stimulatory protein (Gs) couples hormone-receptor interaction to the activation of adenylate cyclase and the generation of cyclic AMP. Studies using frog neuroepithelium indicate that the sense of smell is mediated by a Gs-adenylate cyclase system, and this prompted us to test olfaction ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/322635a0
更新日期:1986-08-14 00:00:00
abstract::Nonrandom chromosome rearrangements, observed in a variety of human and animal tumours, are associated in some cases with enhanced expression or deregulation of cellular oncogenes. Recently, it was shown that normal, diploid rodent cells are neoplastically transformed following transfection with two cooperating oncoge...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/316636a0
更新日期:1985-08-15 00:00:00
abstract::Approximately 2% of colorectal cancer is linked to pre-existing inflammation known as colitis-associated cancer, but most develops in patients without underlying inflammatory bowel disease. Colorectal cancer often follows a genetic pathway whereby loss of the adenomatous polyposis coli (APC) tumour suppressor and acti...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11465
更新日期:2012-11-08 00:00:00
abstract::Neural mechanisms that mediate the ability to make value-guided decisions have received substantial attention in humans and animals1-6. Experiments in animals typically involve long training periods. By contrast, choices in the real world often need to be made between new options spontaneously. It is therefore possibl...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-03115-5
更新日期:2021-01-06 00:00:00
abstract::Leukaemia inhibitory factor (LIF) has a variety of effects on different cell types in vitro, inhibiting the differentiation of embryonic stem cells and promoting the survival and/or proliferation of primitive haematopoietic precursors and primordial germ cells. Here we show that LIF-deficient mice derived by gene targ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/363361a0
更新日期:1993-05-27 00:00:00
abstract::It is of fundamental importance to establish whether there is a limit to how thin a superconducting wire can be, while retaining its superconducting character--and if there is a limit, to determine what sets it. This issue may also be of practical importance in defining the limit to miniaturization of superconducting ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35010060
更新日期:2000-04-27 00:00:00
abstract::Since 1814, when rubella was first described, the origins of the disease and its causative agent, rubella virus (Matonaviridae: Rubivirus), have remained unclear1. Here we describe ruhugu virus and rustrela virus in Africa and Europe, respectively, which are, to our knowledge, the first known relatives of rubella viru...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2812-9
更新日期:2020-10-01 00:00:00
abstract::The 20S cyclosome complex (also known as the anaphase-promoting complex) has ubiquitin ligase activity and is required for mitotic cyclin destruction and sister chromatid separation. The formation and activation of the 20S cyclosome complex is regulated by an unknown mechanism. Here we show that Cut4 (ref. 6) is an es...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/384276a0
更新日期:1996-11-21 00:00:00
abstract::As an approach to the study of the kind of chemical process that might have contributed to the origin of life, attempts have been made to develop purely chemical systems in which oligonucleotides self-replicate. Although performed oligonucleotides have been shown to facilitate the formation of their complements from a...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/327346a0
更新日期:1987-05-28 00:00:00
abstract::Courtship rituals serve to reinforce reproductive barriers between closely related species. Drosophila melanogaster and Drosophila simulans exhibit reproductive isolation, owing in part to the fact that D. melanogaster females produce 7,11-heptacosadiene, a pheromone that promotes courtship in D. melanogaster males bu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-018-0322-9
更新日期:2018-07-01 00:00:00
abstract::An evolutionary view of ageing suggests that mortality may be due to an energy-saving strategy of reduced error regulation in somatic cells. This supports Orgel's 'error catastrophe' hypothesis and offers a new basis for the study of normal and abnormal ageing syndromes and of apparently immortal transformed cell line...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/270301a0
更新日期:1977-11-24 00:00:00
abstract::Most sensory information destined for the neocortex is relayed through the thalamus, where considerable transformation occurs1,2. One means of transformation involves interactions between excitatory thalamocortical neurons that carry data to the cortex and inhibitory neurons of the thalamic reticular nucleus (TRN) tha...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2512-5
更新日期:2020-07-01 00:00:00