Enhanced dihydropyridine receptor channel activity in the presence of ryanodine receptor.


:Excitation-contraction coupling in skeletal muscle involves a voltage sensor in the plasma membrane which, in response to depolarization, causes an intracellular calcium-release channel to open. The skeletal isoform of the ryanodine receptor (RyR-1) functions as the Ca2+-release channel and the dihydropyridine receptor (DHPR) functions as the voltage sensor and also as an L-type Ca2+ channel. Here we examine the possibility that there is a retrograde signal from RyR-1 to the DHPR, using myotubes from mice homozygous for a disrupted RyR-1 gene (dyspedic mice). As expected, we find that there is no excitation-contraction coupling in dyspedic myotubes, but we also find that they have a roughly 30-fold reduction in L-type Ca2+-current density. Injection of dyspedic myotubes with RyR-1 complementary DNA restores excitation-contraction coupling and causes the density of L-type Ca2+ current to rise towards normal. Despite the differences in Ca2+-current magnitude, measurements of charge movement indicate that the density of DHPRs is similar in dyspedic and RyR-1-expressing myotubes. Our results support the possibility of a retrograde signal by which RyR-1 enhances the function of DHPRs as Ca2+ channels.






Nakai J,Dirksen RT,Nguyen HT,Pessah IN,Beam KG,Allen PD




Has Abstract


1996-03-07 00:00:00














  • Unidirectional molecular motor on a gold surface.

    abstract::Molecules capable of mimicking the function of a wide range of mechanical devices have been fabricated, with motors that can induce mechanical movement attracting particular attention. Such molecular motors convert light or chemical energy into directional rotary or linear motion, and are usually prepared and operated...


    pub_type: 杂志文章


    authors: van Delden RA,ter Wiel MK,Pollard MM,Vicario J,Koumura N,Feringa BL

    更新日期:2005-10-27 00:00:00

  • Molybdenum chloride catalysts for Z-selective olefin metathesis reactions.

    abstract::The development of catalyst-controlled stereoselective olefin metathesis processes has been a pivotal recent advance in chemistry. The incorporation of appropriate ligands within complexes based on molybdenum, tungsten and ruthenium has led to reactivity and selectivity levels that were previously inaccessible. Here w...


    pub_type: 杂志文章


    authors: Koh MJ,Nguyen TT,Lam JK,Torker S,Hyvl J,Schrock RR,Hoveyda AH

    更新日期:2017-02-02 00:00:00

  • Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning.

    abstract::Cytosine DNA methylation is important in regulating gene expression and in silencing transposons and other repetitive sequences. Recent genomic studies in Arabidopsis thaliana have revealed that many endogenous genes are methylated either within their promoters or within their transcribed regions, and that gene methyl...


    pub_type: 杂志文章


    authors: Cokus SJ,Feng S,Zhang X,Chen Z,Merriman B,Haudenschild CD,Pradhan S,Nelson SF,Pellegrini M,Jacobsen SE

    更新日期:2008-03-13 00:00:00

  • Earliest sensory nerve fibres are guided to peripheral targets by attractants other than nerve growth factor.

    abstract::Recent studies have shown that developing nerve fibres grow directly to their targets and are guided by specific cues, but the nature of these cues and the mechanism of guidance remain unknown. The growth of sympathetic axons towards an artificial source of nerve growth factor (NGF) in vivo and of sensory neurites up ...


    pub_type: 杂志文章


    authors: Lumsden AG,Davies AM

    更新日期:1983-12-22 00:00:00

  • Retinal astrocytes are immigrants from the optic nerve.

    abstract::The retina in most mammals contains two types of macroglial cells--Müller cells, which span the entire thickness of the retina, and astrocytes, which are mainly confined to the nerve fibre layer. Whereas Müller cells are diffusely distributed in all vertebrate retinae, the presence and distribution of retinal astrocyt...


    pub_type: 杂志文章


    authors: Watanabe T,Raff MC

    更新日期:1988-04-28 00:00:00

  • Horizontal endosymbiont transmission in hydrothermal vent tubeworms.

    abstract::Transmission of obligate bacterial symbionts between generations is vital for the survival of the host. Although the larvae of certain hydrothermal vent tubeworms (Vestimentifera, Siboglinidae) are symbiont-free and possess a transient digestive system, these structures are lost during development, resulting in adult ...


    pub_type: 杂志文章


    authors: Nussbaumer AD,Fisher CR,Bright M

    更新日期:2006-05-18 00:00:00

  • Maximized electron interactions at the magic angle in twisted bilayer graphene.

    abstract::The electronic properties of heterostructures of atomically thin van der Waals crystals can be modified substantially by moiré superlattice potentials from an interlayer twist between crystals1,2. Moiré tuning of the band structure has led to the recent discovery of superconductivity3,4 and correlated insulating phase...


    pub_type: 杂志文章


    authors: Kerelsky A,McGilly LJ,Kennes DM,Xian L,Yankowitz M,Chen S,Watanabe K,Taniguchi T,Hone J,Dean C,Rubio A,Pasupathy AN

    更新日期:2019-08-01 00:00:00

  • A molecular chaperone from a thermophilic archaebacterium is related to the eukaryotic protein t-complex polypeptide-1.

    abstract::There is evidence to suggest that components of archaebacteria are evolutionarily related to cognates in the eukaryotic cytosol. We postulated that the major heat-shock protein of the thermophilic archaebacterium, Sulfolobus shibatae, is a molecular chaperone and that it is related to an as-yet unidentified chaperone ...


    pub_type: 杂志文章


    authors: Trent JD,Nimmesgern E,Wall JS,Hartl FU,Horwich AL

    更新日期:1991-12-12 00:00:00

  • Two levels of protection for the B cell genome during somatic hypermutation.

    abstract::Somatic hypermutation introduces point mutations into immunoglobulin genes in germinal centre B cells during an immune response. The reaction is initiated by cytosine deamination by the activation-induced deaminase (AID) and completed by error-prone processing of the resulting uracils by mismatch and base excision rep...


    pub_type: 杂志文章


    authors: Liu M,Duke JL,Richter DJ,Vinuesa CG,Goodnow CC,Kleinstein SH,Schatz DG

    更新日期:2008-02-14 00:00:00

  • Transient reversion of ras oncogene-induced cell transformation by antibodies specific for amino acid 12 of ras protein.

    abstract::The proteins encoded by the ras oncogene are thought to trigger expression of the transformed phenotype in some types of cancer cells. In human cells, the ras protein family consists of several members including normal (proto-oncogene) and mutant (oncogene) forms. In general, the proto-oncogene forms are thought to be...


    pub_type: 杂志文章


    authors: Feramisco JR,Clark R,Wong G,Arnheim N,Milley R,McCormick F

    更新日期:1985-04-18 00:00:00

  • Structure of the Vdelta domain of a human gammadelta T-cell antigen receptor.

    abstract::Antigen recognition by T lymphocytes is mediated by cell-surface glycoproteins known as T-cell antigen receptors (TCRs). These are composed of alpha and beta, or gamma and delta, polypeptide chains with variable (V) and constant (C) regions. In contrast to alphabeta TCRs, which recognize antigen only as peptide fragme...


    pub_type: 杂志文章


    authors: Li H,Lebedeva MI,Llera AS,Fields BA,Brenner MB,Mariuzza RA

    更新日期:1998-01-29 00:00:00

  • Three mutant insulins in man.

    abstract::We have previously identified a structurally abnormal insulin in the serum and pancreas of a middle-aged man with diabetes mellitus which arose from a leucine for phenylalanine substitution at position 24 or 25 of the insulin B chain; further analysis of the patient's leukocyte DNA showed that one of the patient's ins...


    pub_type: 杂志文章


    authors: Shoelson S,Haneda M,Blix P,Nanjo A,Sanke T,Inouye K,Steiner D,Rubenstein A,Tager H

    更新日期:1983-04-07 00:00:00

  • High-speed countercurrent chromatography.

    abstract::Support-free high-speed countercurrent liquid chromatography provides a rich domain of applications, some beyond reach of conventional liquid chromatography. ...


    pub_type: 杂志文章


    authors: Ito Y

    更新日期:1987-03-26 00:00:00

  • Vernier templating and synthesis of a 12-porphyrin nano-ring.

    abstract::Templates are widely used to arrange molecular components so they can be covalently linked into complex molecules that are not readily accessible by classical synthetic methods. Nature uses sophisticated templates such as the ribosome, whereas chemists use simple ions or small molecules. But as we tackle the synthesis...


    pub_type: 杂志文章


    authors: O'Sullivan MC,Sprafke JK,Kondratuk DV,Rinfray C,Claridge TD,Saywell A,Blunt MO,O'Shea JN,Beton PH,Malfois M,Anderson HL

    更新日期:2011-01-06 00:00:00

  • Energy dependence and reversibility of membrane alterations induced by polyene macrolide antibiotics in Chlorella vulgaris.

    abstract::The requirement of metabolic energy for the interaction of polyene macrolide antibiotics with eukaryotic organisms remains a controversial subject (for review see ref. 1) It has been claimed that the lethal binding of these antibiotics to the sterol target component of the hydrophobic core of the membrane, in accordan...


    pub_type: 杂志文章


    authors: Malewicz B,Borowski E

    更新日期:1979-09-06 00:00:00

  • Physiology: postprandial cardiac hypertrophy in pythons.

    abstract::Oxygen consumption by carnivorous reptiles increases enormously after they have eaten a large meal in order to meet metabolic demands, and this places an extra load on the cardiovascular system. Here we show that there is an extraordinarily rapid 40% increase in ventricular muscle mass in Burmese pythons (Python molur...


    pub_type: 杂志文章


    authors: Andersen JB,Rourke BC,Caiozzo VJ,Bennett AF,Hicks JW

    更新日期:2005-03-03 00:00:00

  • Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells.

    abstract::Our understanding of Alzheimer's disease pathogenesis is currently limited by difficulties in obtaining live neurons from patients and the inability to model the sporadic form of the disease. It may be possible to overcome these challenges by reprogramming primary cells from patients into induced pluripotent stem cell...


    pub_type: 杂志文章


    authors: Israel MA,Yuan SH,Bardy C,Reyna SM,Mu Y,Herrera C,Hefferan MP,Van Gorp S,Nazor KL,Boscolo FS,Carson CT,Laurent LC,Marsala M,Gage FH,Remes AM,Koo EH,Goldstein LS

    更新日期:2012-01-25 00:00:00

  • Single-molecule imaging of transcription dynamics in somatic stem cells.

    abstract::Molecular noise is a natural phenomenon that is inherent to all biological systems1,2. How stochastic processes give rise to the robust outcomes that support tissue homeostasis remains unclear. Here we use single-molecule RNA fluorescent in situ hybridization (smFISH) on mouse stem cells derived from haematopoietic ti...


    pub_type: 杂志文章


    authors: Wheat JC,Sella Y,Willcockson M,Skoultchi AI,Bergman A,Singer RH,Steidl U

    更新日期:2020-07-01 00:00:00

  • Structure of the gating ring from the human large-conductance Ca(2+)-gated K(+) channel.

    abstract::Large-conductance Ca(2+)-gated K(+) (BK) channels are essential for many biological processes such as smooth muscle contraction and neurotransmitter release. This group of channels can be activated synergistically by both voltage and intracellular Ca(2+), with the large carboxy-terminal intracellular portion being res...


    pub_type: 杂志文章


    authors: Wu Y,Yang Y,Ye S,Jiang Y

    更新日期:2010-07-15 00:00:00

  • The Xenopus localized messenger RNA An3 may encode an ATP-dependent RNA helicase.

    abstract::The maternal messenger RNA An3 was originally identified localized to the animal hemisphere of Xenopus laevis oocytes, eggs and early embryos. Xenopus embryos depend on mRNA and protein present in the egg before fertilization (maternal molecules) to provide the information needed for early development. Localization of...


    pub_type: 杂志文章


    authors: Gururajan R,Perry-O'Keefe H,Melton DA,Weeks DL

    更新日期:1991-02-21 00:00:00

  • A 'don't eat me' immune signal protects neuronal connections.

    abstract:: ...


    pub_type: 评论,新闻


    authors: Rivest S

    更新日期:2018-11-01 00:00:00

  • Evidence for a viral superantigen in humans.

    abstract::Superantigens bind class II major histocompatibility proteins and stimulate powerful proliferative responses of T lymphocytes bearing particular V beta sequences as part of their alpha beta antigen receptor. Exogenous bacterial superantigens are responsible for food poisoning and toxic shock syndrome. Murine virus-enc...


    pub_type: 杂志文章


    authors: Lafon M,Lafage M,Martinez-Arends A,Ramirez R,Vuillier F,Charron D,Lotteau V,Scott-Algara D

    更新日期:1992-08-06 00:00:00

  • Progression of autoimmune diabetes driven by avidity maturation of a T-cell population.

    abstract::For unknown reasons, autoimmune diseases such as type 1 diabetes develop after prolonged periods of inflammation of mononuclear cells in target tissues. Here we show that progression of pancreatic islet inflammation to overt diabetes in nonobese diabetic (NOD) mice is driven by the 'avidity maturation' of a prevailing...


    pub_type: 杂志文章


    authors: Amrani A,Verdaguer J,Serra P,Tafuro S,Tan R,Santamaria P

    更新日期:2000-08-17 00:00:00

  • Early Palaeogene temperature evolution of the southwest Pacific Ocean.

    abstract::Relative to the present day, meridional temperature gradients in the Early Eocene age ( approximately 56-53 Myr ago) were unusually low, with slightly warmer equatorial regions but with much warmer subtropical Arctic and mid-latitude climates. By the end of the Eocene epoch ( approximately 34 Myr ago), the first major...


    pub_type: 历史文章,杂志文章


    authors: Bijl PK,Schouten S,Sluijs A,Reichart GJ,Zachos JC,Brinkhuis H

    更新日期:2009-10-08 00:00:00

  • Messenger RNA for the coat protein of tobacco mosaic virus.

    abstract::TMV RNA is not an efficient template for translation of the viral coat protein, in spite of containing nucleotide sequences coding for the protein. Efficient translation requires the prior synthesis within infected cells of a smaller RNA carrying only a portion of the information encoded in the whole genome. ...


    pub_type: 杂志文章


    authors: Hunter TR,Hunt T,Knowland J,Zimmern D

    更新日期:1976-04-29 00:00:00

  • Australopithecus ramidus, a new species of early hominid from Aramis, Ethiopia.

    abstract::Seventeen hominoid fossils recovered from Pliocene strata at Aramis, Middle Awash, Ethiopia make up a series comprising dental, cranial and postcranial specimens dated to around 4.4 million years ago. When compared with Australopithecus afarensis and with modern and fossil apes the Aramis fossil hominids are recognize...


    pub_type: 杂志文章


    authors: White TD,Suwa G,Asfaw B

    更新日期:1994-09-22 00:00:00

  • Triggers, targets and treatments for thrombosis.

    abstract::Thrombosis--localized clotting of the blood--can occur in the arterial or the venous circulation and has a major medical impact. Acute arterial thrombosis is the proximal cause of most cases of myocardial infarction (heart attack) and of about 80% of strokes, collectively the most common cause of death in the develope...


    pub_type: 杂志文章,评审


    authors: Mackman N

    更新日期:2008-02-21 00:00:00

  • Dynamics of nucleosome remodelling by individual ACF complexes.

    abstract::The ATP-dependent chromatin assembly and remodelling factor (ACF) functions to generate regularly spaced nucleosomes, which are required for heritable gene silencing. The mechanism by which ACF mobilizes nucleosomes remains poorly understood. Here we report a single-molecule FRET study that monitors the remodelling of...


    pub_type: 杂志文章


    authors: Blosser TR,Yang JG,Stone MD,Narlikar GJ,Zhuang X

    更新日期:2009-12-24 00:00:00

  • Greater contribution to blood lead from water than from air.

    abstract::Concern about the levels of lead in blood is widespread. There is uncertainty, however, about the relative importance of the various environmental sources. Lead in petrol is widely assumed to be one of the most important sources and air and dust have been identified as the main routes to man. Water is regarded as an i...


    pub_type: 杂志文章


    authors: Elwood PC,Gallacher JE,Phillips KM,Davies BE,Toothill C

    更新日期:1984-07-12 00:00:00

  • Cell-type-specific replication initiation programs set fragility of the FRA3B fragile site.

    abstract::Common fragile sites have long been identified by cytogeneticists as chromosomal regions prone to breakage upon replication stress. They are increasingly recognized to be preferential targets for oncogene-induced DNA damage in pre-neoplastic lesions and hotspots for chromosomal rearrangements in various cancers. Commo...


    pub_type: 杂志文章


    authors: Letessier A,Millot GA,Koundrioukoff S,Lachagès AM,Vogt N,Hansen RS,Malfoy B,Brison O,Debatisse M

    更新日期:2011-02-03 00:00:00