Enhanced dihydropyridine receptor channel activity in the presence of ryanodine receptor.


:Excitation-contraction coupling in skeletal muscle involves a voltage sensor in the plasma membrane which, in response to depolarization, causes an intracellular calcium-release channel to open. The skeletal isoform of the ryanodine receptor (RyR-1) functions as the Ca2+-release channel and the dihydropyridine receptor (DHPR) functions as the voltage sensor and also as an L-type Ca2+ channel. Here we examine the possibility that there is a retrograde signal from RyR-1 to the DHPR, using myotubes from mice homozygous for a disrupted RyR-1 gene (dyspedic mice). As expected, we find that there is no excitation-contraction coupling in dyspedic myotubes, but we also find that they have a roughly 30-fold reduction in L-type Ca2+-current density. Injection of dyspedic myotubes with RyR-1 complementary DNA restores excitation-contraction coupling and causes the density of L-type Ca2+ current to rise towards normal. Despite the differences in Ca2+-current magnitude, measurements of charge movement indicate that the density of DHPRs is similar in dyspedic and RyR-1-expressing myotubes. Our results support the possibility of a retrograde signal by which RyR-1 enhances the function of DHPRs as Ca2+ channels.






Nakai J,Dirksen RT,Nguyen HT,Pessah IN,Beam KG,Allen PD




Has Abstract


1996-03-07 00:00:00














  • Atlantic overturning responses to Late Pleistocene climate forcings.

    abstract::The factors driving glacial changes in ocean overturning circulation are not well understood. On the basis of a comparison of 20 climate variables over the past four glacial cycles, the SPECMAP project proposed that summer insolation at high northern latitudes (that is, Milankovitch forcing) drives the same sequence o...


    pub_type: 历史文章,杂志文章


    authors: Lisiecki LE,Raymo ME,Curry WB

    更新日期:2008-11-06 00:00:00

  • Structure of C3b reveals conformational changes that underlie complement activity.

    abstract::Resistance to infection and clearance of cell debris in mammals depend on the activation of the complement system, which is an important component of innate and adaptive immunity. Central to the complement system is the activated form of C3, called C3b, which attaches covalently to target surfaces to amplify complemen...


    pub_type: 杂志文章


    authors: Janssen BJ,Christodoulidou A,McCarthy A,Lambris JD,Gros P

    更新日期:2006-11-09 00:00:00

  • Crystal structure at 2.8 A resolution of a soluble form of the cell adhesion molecule CD2.

    abstract::The crystal structure of a soluble form of the T lymphocyte antigen CD2 provides the first complete view of the extracellular region of a cell adhesion molecule. The topology of the molecule, which comprises two immunoglobulin-like domains, is the same as that of the first two domains of CD4 but the relative domain or...


    pub_type: 杂志文章


    authors: Jones EY,Davis SJ,Williams AF,Harlos K,Stuart DI

    更新日期:1992-11-19 00:00:00

  • Direct human influence on atmospheric CO2 seasonality from increased cropland productivity.

    abstract::Ground- and aircraft-based measurements show that the seasonal amplitude of Northern Hemisphere atmospheric carbon dioxide (CO2) concentrations has increased by as much as 50 per cent over the past 50 years. This increase has been linked to changes in temperate, boreal and arctic ecosystem properties and processes suc...


    pub_type: 杂志文章


    authors: Gray JM,Frolking S,Kort EA,Ray DK,Kucharik CJ,Ramankutty N,Friedl MA

    更新日期:2014-11-20 00:00:00

  • Wave and defect dynamics in nonlinear photonic quasicrystals.

    abstract::Quasicrystals are unique structures with long-range order but no periodicity. Their properties have intrigued scientists ever since their discovery and initial theoretical analysis. The lack of periodicity excludes the possibility of describing quasicrystal structures with well-established analytical tools, including ...


    pub_type: 杂志文章


    authors: Freedman B,Bartal G,Segev M,Lifshitz R,Christodoulides DN,Fleischer JW

    更新日期:2006-04-27 00:00:00

  • HIF-1α metabolically controls collagen synthesis and modification in chondrocytes.

    abstract::Endochondral ossification, an important process in vertebrate bone formation, is highly dependent on correct functioning of growth plate chondrocytes1. Proliferation of these cells determines longitudinal bone growth and the matrix deposited provides a scaffold for future bone formation. However, these two energy-depe...


    pub_type: 杂志文章


    authors: Stegen S,Laperre K,Eelen G,Rinaldi G,Fraisl P,Torrekens S,Van Looveren R,Loopmans S,Bultynck G,Vinckier S,Meersman F,Maxwell PH,Rai J,Weis M,Eyre DR,Ghesquière B,Fendt SM,Carmeliet P,Carmeliet G

    更新日期:2019-01-01 00:00:00

  • Structure of a C-type mannose-binding protein complexed with an oligosaccharide.

    abstract::C-type (Ca(2+)-dependent) animal lectins such as mannose-binding proteins mediate many cell-surface carbohydrate-recognition events. The crystal structure at 1.7 A resolution of the carbohydrate-recognition domain of rat mannose-binding protein complexed with an oligomannose asparaginyl-oligosaccharide reveals that Ca...


    pub_type: 杂志文章


    authors: Weis WI,Drickamer K,Hendrickson WA

    更新日期:1992-11-12 00:00:00

  • Genome-wide non-mendelian inheritance of extra-genomic information in Arabidopsis.

    abstract::A fundamental tenet of classical mendelian genetics is that allelic information is stably inherited from one generation to the next, resulting in predictable segregation patterns of differing alleles. Although several exceptions to this principle are known, all represent specialized cases that are mechanistically rest...


    pub_type: 杂志文章


    authors: Lolle SJ,Victor JL,Young JM,Pruitt RE

    更新日期:2005-03-24 00:00:00

  • A self-renewing multipotential stem cell in embryonic rat cerebral cortex.

    abstract::Neuroectoderm cells in the cortical ventricular zone generate many diverse cell types, maintain the ventricular zone during embryonic life and create another germinal layer, the subventricular zone, which persists into adulthood. In other vertebrate tissues, including skin, intestine, blood and neural crest, stem cell...


    pub_type: 杂志文章


    authors: Davis AA,Temple S

    更新日期:1994-11-17 00:00:00

  • Retinoid X receptor is an auxiliary protein for thyroid hormone and retinoic acid receptors.

    abstract::Thyroid hormones and retinoic acid function through nuclear receptors that belong to the steroid/thyroid-hormone receptor superfamily. Thyroid hormone receptors (TRs) and retinoic acid receptors (RARs) require auxiliary nuclear proteins for efficient DNA binding. Here we report that retinoid X receptors RXR alpha is o...


    pub_type: 杂志文章


    authors: Zhang XK,Hoffmann B,Tran PB,Graupner G,Pfahl M

    更新日期:1992-01-30 00:00:00

  • Structural basis of family-wide Rab GTPase recognition by rabenosyn-5.

    abstract::Rab GTPases regulate all stages of membrane trafficking, including vesicle budding, cargo sorting, transport, tethering and fusion. In the inactive (GDP-bound) conformation, accessory factors facilitate the targeting of Rab GTPases to intracellular compartments. After nucleotide exchange to the active (GTP-bound) conf...


    pub_type: 杂志文章


    authors: Eathiraj S,Pan X,Ritacco C,Lambright DG

    更新日期:2005-07-21 00:00:00

  • Thrombospondin binds falciparum malaria parasitized erythrocytes and may mediate cytoadherence.

    abstract::Plasmodium falciparum infected erythrocytes containing mature trophozoites and schizonts sequester along venular endothelium and are not in the peripheral circulation of patients with malaria. Knobs appear on infected erythrocytes and are the points of attachment to endothelium. Sequestration may protect the parasite ...


    pub_type: 杂志文章


    authors: Roberts DD,Sherwood JA,Spitalnik SL,Panton LJ,Howard RJ,Dixit VM,Frazier WA,Miller LH,Ginsburg V

    更新日期:1985-11-07 00:00:00

  • Isolation of the dorsal locus of Drosophila.

    abstract::The establishment of embryonic polarity is a crucial step in pattern formation and morphogenesis. In the fruitfly Drosophila melanogaster, embryonic polarity depends primarily on genes expressed in the female during oogenesis. Mutations in these 'maternal effect' genes can lead to major disruptions in normal pattern f...


    pub_type: 杂志文章


    authors: Steward R,McNally FJ,Schedl P

    更新日期:1984-09-20 00:00:00

  • A record of planet migration in the main asteroid belt.

    abstract::The main asteroid belt lies between the orbits of Mars and Jupiter, but the region is not uniformly filled with asteroids. There are gaps, known as the Kirkwood gaps, in distinct locations that are associated with orbital resonances with the giant planets; asteroids placed in these locations will follow chaotic orbits...


    pub_type: 杂志文章


    authors: Minton DA,Malhotra R

    更新日期:2009-02-26 00:00:00

  • Positron emission tomography after MPTP: observations relating to the cause of Parkinson's disease.

    abstract::The dopa analogue 6-fluorodopa (6-FD) used with positron emission tomography (PET) allows in vivo visualization of dopamine and its metabolites in nigrostriatal nerve endings. We have now found abnormal 6-FD scans in four subjects exposed to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). None had parkinsonism. T...


    pub_type: 杂志文章


    authors: Calne DB,Langston JW,Martin WR,Stoessl AJ,Ruth TJ,Adam MJ,Pate BD,Schulzer M

    更新日期:1985-09-19 00:00:00

  • No mixing of superconductivity and antiferromagnetism in a high-temperature superconductor.

    abstract::There is still no universally accepted theory of high-temperature superconductivity. Most models assume that doping creates 'holes' in the valence band of an insulating, antiferromagnetic 'parent' compound, and that antiferromagnetism and high-temperature superconductivity are intimately related. If their respective e...


    pub_type: 杂志文章


    authors: Bozovic I,Logvenov G,Verhoeven MA,Caputo P,Goldobin E,Geballe TH

    更新日期:2003-04-24 00:00:00

  • Enzymes trapped and zapped for use outside cells.

    abstract:: ...


    pub_type: 评论,新闻


    authors: Narayan A

    更新日期:2019-03-01 00:00:00

  • Mitochondrial disorders as windows into an ancient organelle.

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    pub_type: 杂志文章,评审


    authors: Vafai SB,Mootha VK

    更新日期:2012-11-15 00:00:00

  • Transformation and diversification in early mammal evolution.

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    pub_type: 历史文章,杂志文章,评审


    authors: Luo ZX

    更新日期:2007-12-13 00:00:00

  • Overcoming EGFR(T790M) and EGFR(C797S) resistance with mutant-selective allosteric inhibitors.

    abstract::The epidermal growth factor receptor (EGFR)-directed tyrosine kinase inhibitors (TKIs) gefitinib, erlotinib and afatinib are approved treatments for non-small cell lung cancers harbouring activating mutations in the EGFR kinase, but resistance arises rapidly, most frequently owing to the secondary T790M mutation withi...


    pub_type: 杂志文章


    authors: Jia Y,Yun CH,Park E,Ercan D,Manuia M,Juarez J,Xu C,Rhee K,Chen T,Zhang H,Palakurthi S,Jang J,Lelais G,DiDonato M,Bursulaya B,Michellys PY,Epple R,Marsilje TH,McNeill M,Lu W,Harris J,Bender S,Wong KK,Jänne PA

    更新日期:2016-06-02 00:00:00

  • A motif in the alphabeta T-cell receptor controls positive selection by modulating ERK activity.

    abstract::Positive selection allows thymocytes that recognize an individual's own major histocompatibility complex (self-MHC) molecules to survive and differentiate, whereas negative selection removes overtly self-reactive thymocytes. Although both forms of thymic selection are mediated by the alphabeta T-cell receptor (TCR) an...


    pub_type: 杂志文章


    authors: Werlen G,Hausmann B,Palmer E

    更新日期:2000-07-27 00:00:00

  • Clinical efficacy of a RAF inhibitor needs broad target blockade in BRAF-mutant melanoma.

    abstract::B-RAF is the most frequently mutated protein kinase in human cancers. The finding that oncogenic mutations in BRAF are common in melanoma, followed by the demonstration that these tumours are dependent on the RAF/MEK/ERK pathway, offered hope that inhibition of B-RAF kinase activity could benefit melanoma patients. He...


    pub_type: 杂志文章


    authors: Bollag G,Hirth P,Tsai J,Zhang J,Ibrahim PN,Cho H,Spevak W,Zhang C,Zhang Y,Habets G,Burton EA,Wong B,Tsang G,West BL,Powell B,Shellooe R,Marimuthu A,Nguyen H,Zhang KY,Artis DR,Schlessinger J,Su F,Higgins B,Iy

    更新日期:2010-09-30 00:00:00

  • Anthropogenic greenhouse gas contribution to flood risk in England and Wales in autumn 2000.

    abstract::Interest in attributing the risk of damaging weather-related events to anthropogenic climate change is increasing. Yet climate models used to study the attribution problem typically do not resolve the weather systems associated with damaging events such as the UK floods of October and November 2000. Occurring during t...


    pub_type: 杂志文章


    authors: Pall P,Aina T,Stone DA,Stott PA,Nozawa T,Hilberts AG,Lohmann D,Allen MR

    更新日期:2011-02-17 00:00:00

  • Mfsd2a is critical for the formation and function of the blood-brain barrier.

    abstract::The central nervous system (CNS) requires a tightly controlled environment free of toxins and pathogens to provide the proper chemical composition for neural function. This environment is maintained by the 'blood-brain barrier' (BBB), which is composed of blood vessels whose endothelial cells display specialized tight...


    pub_type: 杂志文章


    authors: Ben-Zvi A,Lacoste B,Kur E,Andreone BJ,Mayshar Y,Yan H,Gu C

    更新日期:2014-05-22 00:00:00

  • Genome-wide association study of 14,000 cases of seven common diseases and 3,000 shared controls.

    abstract::There is increasing evidence that genome-wide association (GWA) studies represent a powerful approach to the identification of genes involved in common human diseases. We describe a joint GWA study (using the Affymetrix GeneChip 500K Mapping Array Set) undertaken in the British population, which has examined approxima...


    pub_type: 杂志文章,多中心研究


    authors: Wellcome Trust Case Control Consortium.

    更新日期:2007-06-07 00:00:00

  • Independent rate and temporal coding in hippocampal pyramidal cells.

    abstract::In the brain, hippocampal pyramidal cells use temporal as well as rate coding to signal spatial aspects of the animal's environment or behaviour. The temporal code takes the form of a phase relationship to the concurrent cycle of the hippocampal electroencephalogram theta rhythm. These two codes could each represent a...


    pub_type: 杂志文章


    authors: Huxter J,Burgess N,O'Keefe J

    更新日期:2003-10-23 00:00:00

  • S-phase feedback control in budding yeast independent of tyrosine phosphorylation of p34cdc28.

    abstract::In somatic cells, entry into mitosis depends on the completion of DNA synthesis. This dependency is established by S-phase feedback controls that arrest cell division when damaged or unreplicated DNA is present. In the fission yeast Schizosaccharomyces pombe, mutations that interfere with the phosphorylation of tyrosi...


    pub_type: 杂志文章


    authors: Sorger PK,Murray AW

    更新日期:1992-01-23 00:00:00

  • Unusually dynamic sex roles in a fish.

    abstract::Sex roles are typically thought of as being fixed for a given species. In most animals males compete for females, whereas the females are more reluctant to mate. Therefore sexual selection usually acts most strongly on males. This is explained by males having a higher potential reproductive rate than females, leading ...


    pub_type: 杂志文章


    authors: Forsgren E,Amundsen T,Borg AA,Bjelvenmark J

    更新日期:2004-06-03 00:00:00

  • Reduction of disulphide bonds unmasks potent antimicrobial activity of human β-defensin 1.

    abstract::Human epithelia are permanently challenged by bacteria and fungi, including commensal and pathogenic microbiota. In the gut, the fraction of strict anaerobes increases from proximal to distal, reaching 99% of bacterial species in the colon. At colonic mucosa, oxygen partial pressure is below 25% of airborne oxygen con...


    pub_type: 杂志文章


    authors: Schroeder BO,Wu Z,Nuding S,Groscurth S,Marcinowski M,Beisner J,Buchner J,Schaller M,Stange EF,Wehkamp J

    更新日期:2011-01-20 00:00:00

  • The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1.

    abstract::Many tyrosine kinases, including the receptors for hormones such as epidermal growth factor (EGF), nerve growth factor and insulin, transmit intracellular signals through Ras proteins. Ligand binding to such receptors stimulates Ras guanine-nucleotide-exchange activity and increases the level of GTP-bound Ras, suggest...


    pub_type: 杂志文章


    authors: Rozakis-Adcock M,Fernley R,Wade J,Pawson T,Bowtell D

    更新日期:1993-05-06 00:00:00