Abstract：
:We have shown previously that chromosome VI of Saccharomyces cerevisiae contains nine origins of DNA replication that differ in initiation frequency and replicate sequentially during the S phase of the cell cycle. Here we show that there are links between activation of these multiple origins and regulation of S-phase progression. We study the effects of a DNA-damaging agent, methyl methane sulphonate (MMS), and of mutations in checkpoint genes such as rad53 on the activity of origins, measured by two-dimensional gel analysis, and on cell-cycle progression, measured by fluorescence-activated cell sorting. We find that when MMS slows down S-phase progression it also selectively blocks initiation from late origins. A rad53 mutation enhances late and/or inefficient origins and releases the initiation block by MMS. Mutation of rad53 also results in a late origin becoming early replicating. We conclude that rad53 regulates the timing of initiation of replication from late origins during normal cell growth and blocks initiation from late origins in MMS-treated cells. rad53 is, therefore, involved in the cell's surveillance of S-phase progression. We also find that orc2, which encodes subunit 2 of the origin-recognition complex, is involved in suppression of late origins.
journal_name
Naturejournal_title
Natureauthors
Shirahige K,Hori Y,Shiraishi K,Yamashita M,Takahashi K,Obuse C,Tsurimoto T,Yoshikawa Hdoi
10.1038/27007subject
Has Abstractpub_date
1998-10-08 00:00:00pages
618-21issue
6702eissn
0028-0836issn
1476-4687journal_volume
395pub_type
杂志文章相关文献
NATURE文献大全abstract：:Cells deficient in the Brca1 and Brca2 genes have reduced capacity to repair DNA double-strand breaks by homologous recombination and consequently are hypersensitive to DNA-damaging agents, including cisplatin and poly(ADP-ribose) polymerase (PARP) inhibitors. Here we show that loss of the MLL3/4 complex protein, PTIP...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature18325
更新日期：2016-07-21 00:00:00
abstract：:The proton-activated chloride channel (PAC) is active across a wide range of mammalian cells and is involved in acid-induced cell death and tissue injury1-3. PAC has recently been shown to represent a novel and evolutionarily conserved protein family4,5. Here we present two cryo-electron microscopy structures of human...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-2875-7
更新日期：2020-12-01 00:00:00
abstract：:Exploiters (parasites and predators) are thought to play a significant role in diversification, and ultimately speciation, of their hosts or prey. Exploiters may drive sympatric (within-population) diversification if there are a variety of exploiter-resistance strategies or fitness costs associated with exploiter resi...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature01164
更新日期：2002-12-05 00:00:00
abstract：:Long-term depletion of the stratospheric ozone layer contributes to an increase in terrestrial solar ultraviolet-B radiation. This has deleterious effects on living organisms, such as DNA damage. When exposed to elevated ultraviolet-B radiation (UV-B; 280-315 nm), plants display a wide variety of physiological and mor...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35017595
更新日期：2000-07-06 00:00:00
abstract：:Recognition of a functional 3' splice site in pre-mRNA splicing requires a heterodimer of the proteins U2AF65/U2AF35. U2AF65 binds to RNA at the polypyrimidine tract, whereas U2AF35 is thought to interact through its arginine/serine-rich (RS) domain with other RS-domain-containing factors bound at the 5' splice site, ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/41137
更新日期：1997-07-24 00:00:00
abstract：:Growth factors such as platelet-derived growth factor (PDGF) elicit the transcriptional activation of a large number of immediate early genes (many of which encode transcription factors), and ultimately DNA synthesis. Both AP1 and Myc are activated in fibroblasts in response to growth factor stimulation, and various e...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/378509a0
更新日期：1995-11-30 00:00:00
abstract：:Gradual accumulation of evidence is thought to be fundamental for decision-making, and its neural correlates have been found in several brain regions. Here we develop a generalizable method to measure tuning curves that specify the relationship between neural responses and mentally accumulated evidence, and apply it t...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature14066
更新日期：2015-04-09 00:00:00
abstract：:In Parkinson's disease, brain dopamine neurons degenerate most prominently in the substantia nigra. Neurotrophic factors promote survival, differentiation and maintenance of neurons in developing and adult vertebrate nervous system. The most potent neurotrophic factor for dopamine neurons described so far is the glial...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature05957
更新日期：2007-07-05 00:00:00
abstract：:The biochemical response to food intake must be precisely regulated. Because ingested sugars and fats can feed into many anabolic and catabolic pathways1, how our bodies handle nutrients depends on strategically positioned metabolic sensors that link the intrinsic nutritional value of a meal with intermediary metaboli...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-018-0849-9
更新日期：2019-02-01 00:00:00
abstract：:The formation of precise connections between retina and lateral geniculate nucleus (LGN) involves the activity-dependent elimination of some synapses, with strengthening and retention of others. Here we show that the major histocompatibility complex (MHC) class I molecule H2-D(b) is necessary and sufficient for synaps...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13154
更新日期：2014-05-08 00:00:00
abstract：:Organic photovoltaic cells are now approaching commercially viable efficiencies, particularly for applications that make use of their unique potential for flexibility and semitransparency1-3. However, their reliability remains a major concern, as even the most stable devices reported so far degrade within only a few y...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1544-1
更新日期：2019-09-01 00:00:00
abstract：:Support-free high-speed countercurrent liquid chromatography provides a rich domain of applications, some beyond reach of conventional liquid chromatography. ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/326419a0
更新日期：1987-03-26 00:00:00
abstract：:The onsert of neuronal differentiation is characterised by intensive neurite growth; because microtubule formation is strictly required during this process, in vitro assembly of the tubulin present in the rat brain has been studied at different stages of development: the rate of assembly is very slow in the early stag...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/284353a0
更新日期：1980-03-27 00:00:00
abstract：:Memory formation is a multi-stage process that initially requires cellular consolidation in the hippocampus, after which memories are downloaded to the cortex for maintenance, in a process termed systems consolidation. Epigenetic mechanisms regulate both types of consolidation, but histone variant exchange, in which c...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13707
更新日期：2014-11-27 00:00:00
abstract：:A powerful way to discover key genes with causal roles in oncogenesis is to identify genomic regions that undergo frequent alteration in human cancers. Here we present high-resolution analyses of somatic copy-number alterations (SCNAs) from 3,131 cancer specimens, belonging largely to 26 histological types. We identif...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature08822
更新日期：2010-02-18 00:00:00
abstract：:To artists, the face is a mirror of the soul. To biologists, the face reflects remarkable structural diversity--think of bulldogs and wolfhounds or galapagos finches. How do such variations in skeletal form arise? Do the same mechanisms control skeletogenesis elsewhere in the body? The answers lie in the molecular mac...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/nature01656
更新日期：2003-05-15 00:00:00
abstract：:The Duchenne muscular dystrophy (DMD) locus has been localized to the short arm of the human X chromosome (Xp21) by detection of structural abnormalities and by genetic linkage studies. A library highly enriched for human DNA from Xp21 was constructed using DNA isolated from a male patient who had a visible deletion a...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/316842a0
更新日期：1985-08-29 00:00:00
abstract：:A thermal light-emitting source, such as a black body or the incandescent filament of a light bulb, is often presented as a typical example of an incoherent source and is in marked contrast to a laser. Whereas a laser is highly monochromatic and very directional, a thermal source has a broad spectrum and is usually qu...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/416061a
更新日期：2002-03-07 00:00:00
abstract：:Osteoclasts are the only somatic cells with bone-resorbing capacity and, as such, they have a critical role not only in normal bone homeostasis (called 'bone remodelling') but also in the pathogenesis of bone destructive disorders such as rheumatoid arthritis and osteoporosis. A major focus of research in the field ha...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature07713
更新日期：2009-03-26 00:00:00
abstract：:In associative learning, the activated neurones undergo a variety of concomitant functional alterations--increases or decreases of firing activity and modifications of membrane potential or resistance and of synaptic responsiveness. Synaptic transmission which can be strengthened only when there is paired activity in ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/290413a0
更新日期：1981-04-02 00:00:00
abstract：:A T-cell-mediated immune response is initiated by the T-cell receptor (TCR) interacting with peptide-bound major histocompatibility complex (pMHC) on an infected cell. The mechanism by which this interaction triggers intracellular phosphorylation of the TCR, which lacks a kinase domain, remains poorly understood. Here...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature11220
更新日期：2012-07-05 00:00:00
abstract：:Sleep has been assumed to be necessary for development and to be a vital function in mammals and other animals. However, Lyamin et al. claim that in bottlenose dolphins (Tursiops truncatus) and killer whales (Orcinus orca), neonates and their mothers show almost no sleep behaviour for the first month after birth; this...
journal_title：Nature
pub_type： 评论,杂志文章
doi：10.1038/nature04898
更新日期：2006-06-22 00:00:00
abstract：:The signal recognition particle (SRP) consists of one RNA and six protein subunits. The N-terminal domain of the 54K subunit contains a putative GTP-binding site, whereas the C-terminal domain binds signal sequences and SRP RNA. Binding of SRP to the signal sequence as it emerges from the ribosome creates a cytosolic ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/366351a0
更新日期：1993-11-25 00:00:00
abstract：:Atrial natriuretic peptide (ANP) is a polypeptide hormone whose effects include the induction of diuresis, natriuresis and vasorelaxation. One of the earliest events following binding of ANP to receptors on target cells is an increase in cyclic GMP concentration, indicating that this nucleotide might act as a mediator...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/338078a0
更新日期：1989-03-02 00:00:00
abstract：:Recent studies have shown that normal cellular sequences on chromosome 13 are lost during the development of retinoblastomas and that sequences on chromosome 11 are similarly lost during the development of Wilms' kidney tumours and embryonal tumours. Cells from these tumors have been found to contain either the patern...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/318377a0
更新日期：1985-11-28 00:00:00
abstract：:Since the first aeroplane flight more than 100 years ago, aeroplanes have been propelled using moving surfaces such as propellers and turbines. Most have been powered by fossil-fuel combustion. Electroaerodynamics, in which electrical forces accelerate ions in a fluid1,2, has been proposed as an alternative method of ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-018-0707-9
更新日期：2018-11-01 00:00:00
abstract：:How bacteria regulate cell cycle progression at a molecular level is a fundamental but poorly understood problem. In Caulobacter crescentus, two-component signal transduction proteins are crucial for cell cycle regulation, but the connectivity of regulators involved has remained elusive and key factors are unidentifie...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature05321
更新日期：2006-12-14 00:00:00
abstract：:Highly collimated parsec-scale jets, which are generally linked to the presence of an accretion disk, are commonly observed in low-mass young stellar objects. In the past two decades, a few of these jets have been directly (or indirectly) observed from higher-mass (larger than eight solar masses) young stellar objects...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature25189
更新日期：2018-02-15 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41586-020-2064-8
更新日期：2020-03-01 00:00:00
abstract：:Ice flow along land-terminating margins of the Greenland Ice Sheet (GIS) varies considerably in response to fluctuating inputs of surface meltwater to the bed of the ice sheet. Such inputs lubricate the ice-bed interface, transiently speeding up the flow of ice. Greater melting results in faster ice motion during summ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature15722
更新日期：2015-10-29 00:00:00
