Abstract:
:The type 1 human immunodeficiency virus (HIV-1) contains a conical capsid comprising approximately 1,500 CA protein subunits, which organizes the viral RNA genome for uncoating and replication in a new host cell. In vitro, CA spontaneously assembles into helical tubes and cones that resemble authentic viral capsids. Here we describe electron cryo-microscopy and image reconstructions of CA tubes from six different helical families. In spite of their polymorphism, all tubes are composed of hexameric rings of CA arranged with approximate local p6 lattice symmetry. Crystal structures of the two CA domains were 'docked' into the reconstructed density, which showed that the amino-terminal domains form the hexameric rings and the carboxy-terminal dimerization domains connect each ring to six neighbours. We propose a molecular model for the HIV-1 capsid that follows the principles of a fullerene cone, in which the body of the cone is composed of curved hexagonal arrays of CA rings and the ends are closed by inclusion of 12 pentagonal 'defects'.
journal_name
Naturejournal_title
Natureauthors
Li S,Hill CP,Sundquist WI,Finch JTdoi
10.1038/35030177keywords:
subject
Has Abstractpub_date
2000-09-21 00:00:00pages
409-13issue
6802eissn
0028-0836issn
1476-4687journal_volume
407pub_type
杂志文章相关文献
NATURE文献大全abstract::D-type cyclins, in association with the cyclin-dependent kinases Cdk4 or Cdk6, promote progression through the G1 phase of the cell cycle by phosphorylating the retinoblastoma protein (RB). The activities of Cdk4 and Cdk6 are constrained by inhibitors such as p16, the product of the CDKN2 gene on human chromosome 9p21...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/375503a0
更新日期:1995-06-08 00:00:00
abstract::Mutations in the p53 tumour-suppressor gene are the most frequently observed genetic lesions in human cancers. To investigate the role of the p53 gene in mammalian development and tumorigenesis, a null mutation was introduced into the gene by homologous recombination in murine embryonic stem cells. Mice homozygous for...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/356215a0
更新日期:1992-03-19 00:00:00
abstract::RNA polymerase II (RNAP II) is responsible for transcribing all messenger RNAs in eukaryotic cells during a highly regulated process that is conserved from yeast to human, and that serves as a central control point for cellular function. Here we investigate the transcription dynamics of single RNAP II molecules from S...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature05701
更新日期:2007-04-12 00:00:00
abstract::We describe an integrated physical, genetic and cytogenetic map of human chromosome 16 comprising both a low-resolution megaYAC map and a high-resolution cosmid contig/miniYAC map, which provides nearly complete coverage of the euchromatic arms of the chromosome. The physical map is anchored to a high-resolution cytog...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/377335a0
更新日期:1995-09-28 00:00:00
abstract::The factors driving glacial changes in ocean overturning circulation are not well understood. On the basis of a comparison of 20 climate variables over the past four glacial cycles, the SPECMAP project proposed that summer insolation at high northern latitudes (that is, Milankovitch forcing) drives the same sequence o...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/nature07425
更新日期:2008-11-06 00:00:00
abstract::A product encoded at the claret locus in Drosophila is needed for normal chromosome segregation in meiosis in females and in early mitotic divisions of the embryo. The predicted amino-acid sequence of the segregation protein was shown recently to be strikingly similar to Drosophila kinesin heavy chain. We have express...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/347780a0
更新日期:1990-10-25 00:00:00
abstract::The nucleotides immediately surrounding intron/exon junctions of genes transcribed by RNA polymerase B can be derived from 'consensus' sequences for donor and acceptor splice sites by only a few base changes. Studies in vivo have underlined the importance of these junction nucleotides for splicing. In higher eukaryote...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/315430a0
更新日期:1985-05-30 00:00:00
abstract::When simian virus 40 (SV40) infects host cells, the viral genes are expressed in two phases. Early in infection, the virus genome is transcribed and translated to yield two related proteins, 'large T' and 'small t' antigens. Later the rate of synthesis of the early messenger RNA falls and the viral genes coding for th...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/289810a0
更新日期:1981-02-26 00:00:00
abstract::Ten eleven translocation (TET) enzymes, including TET1, TET2 and TET3, convert 5-methylcytosine to 5-hydroxymethylcytosine and regulate gene transcription. However, the molecular mechanism by which TET family enzymes regulate gene transcription remains elusive. Using protein affinity purification, here we search for f...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11742
更新日期:2013-01-24 00:00:00
abstract::p60src, the transforming protein of Rous sarcoma virus (RSV), is a protein kinase that has a strict specificity for tyrosine. The phosphorylation of cellular proteins by p60src (ref. 4) results in transformation. Recently, Barker and Dayhoff discovered that residues 259-485 of p60src have 22% sequence identity with re...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/310589a0
更新日期:1984-08-16 00:00:00
abstract::A rapid decrease in shear velocity in the suboceanic mantle is used to infer the thickness of the lithosphere. It is proposed that new and highly precise group velocity data constrain the solutions and imply a thickness near 70 km. ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/226330a0
更新日期:1970-04-25 00:00:00
abstract::The direct involvement of the pituitary-adrenal axis in birth has been well established, at least in sheep, and its removal prolongs pregnancy. As part of the process the fetal sheep adrenal grows rapidly during the 10-15 d prepartum and is associated with a large rise in the plasma corticosteroid concentration. This ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/284616a0
更新日期:1980-04-17 00:00:00
abstract::Sexual differences in the extent and type of parental care lie at the heart of sexual selection theory, and evolution resulting from parental conflict has produced some striking behavioural and morphological adaptations. In a study of male pregnancy in Gulf pipefish, Paczolt and Jones showed that more eggs were transf...
journal_title:Nature
pub_type: 评论,杂志文章
doi:10.1038/nature09275
更新日期:2010-08-26 00:00:00
abstract::For plants, the sensing of light in the environment is as important as vision is for animals. Fluctuations in light can be crucial to competition and survival. One way plants sense light is through the phytochromes, a small family of diverse photochromic protein photoreceptors whose origins have been traced to the pho...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/35036500
更新日期:2000-10-05 00:00:00
abstract::Effective interaction between T cells and their targets requires that recognition of specific antigen be coordinated with increased cell-cell adhesion. We show that antigen-receptor cross-linking increases the strength of the adhesion mechanism between lymphocyte function-associated molecule-1 (LFA-1) and intercellula...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/341619a0
更新日期:1989-10-19 00:00:00
abstract::Numerous studies have suggested that biodiversity reduces variability in ecosystem productivity through compensatory effects; that is, a species increases in its abundance in response to the reduction of another in a fluctuating environment. But this view has been challenged on several grounds. Because most studies ha...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02850
更新日期:2004-09-09 00:00:00
abstract::Spinal cord injury disrupts the communication between the brain and the spinal circuits that orchestrate movement. To bypass the lesion, brain-computer interfaces have directly linked cortical activity to electrical stimulation of muscles, and have thus restored grasping abilities after hand paralysis. Theoretically, ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature20118
更新日期:2016-11-10 00:00:00
abstract::Sea ice and dust flux increased greatly in the Southern Ocean during the last glacial period. Palaeorecords provide contradictory evidence about marine productivity in this region, but beyond one glacial cycle, data were sparse. Here we present continuous chemical proxy data spanning the last eight glacial cycles (740...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04614
更新日期:2006-03-23 00:00:00
abstract::Grid cells in the entorhinal cortex of freely moving rats provide a strikingly periodic representation of self-location which is indicative of very specific computational mechanisms. However, the existence of grid cells in humans and their distribution throughout the brain are unknown. Here we show that the preferred ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature08704
更新日期:2010-02-04 00:00:00
abstract::During anaphase identical sister chromatids separate and move towards opposite poles of the mitotic spindle. In the spindle, kinetochore microtubules have their plus ends embedded in the kinetochore and their minus ends at the spindle pole. Two models have been proposed to account for the movement of chromatids during...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02256
更新日期:2004-01-22 00:00:00
abstract::Data collected at approximately 60 Global Positioning System (GPS) sites in southeast Asia show the crustal deformation caused by the 26 December 2004 Sumatra-Andaman earthquake at an unprecedented large scale. Small but significant co-seismic jumps are clearly detected more than 3,000 km from the earthquake epicentre...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03937
更新日期:2005-07-14 00:00:00
abstract::Clear cell renal cell carcinoma (ccRCC), the most common form of kidney cancer, is characterized by elevated glycogen levels and fat deposition. These consistent metabolic alterations are associated with normoxic stabilization of hypoxia-inducible factors (HIFs) secondary to von Hippel-Lindau (VHL) mutations that occu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature13557
更新日期:2014-09-11 00:00:00
abstract::Spectacular fossils from the Early Cretaceous Jehol Group of northeastern China have greatly expanded our knowledge of the diversity and palaeobiology of dinosaurs and early birds, and contributed to our understanding of the origin of birds, of flight, and of feathers. Pennaceous (vaned) feathers and integumentary fil...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature08740
更新日期:2010-02-25 00:00:00
abstract::Ia antigens are membrane-bound glycoproteins that play a part in antigen recognition and subsequent cell-cell interactions in the immune response. In the mouse they are coded for by the I region of the major histocompatibility complex H-2 and have been demonstrated on B lymphocytes, monocytes, activated T cells, macro...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/303426a0
更新日期:1983-06-02 00:00:00
abstract::De novo engineering of gene circuits inside cells is extremely difficult, and efforts to realize predictable and robust performance must deal with noise in gene expression and variation in phenotypes between cells. Here we demonstrate that by coupling gene expression to cell survival and death using cell-cell communic...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02491
更新日期:2004-04-22 00:00:00
abstract::Techniques to facilitate controlled interactions between single photons and atoms are now being actively explored. These techniques are important for the practical realization of quantum networks, in which multiple memory nodes that utilize atoms for generation, storage and processing of quantum states are connected b...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04327
更新日期:2005-12-08 00:00:00
abstract::The detection of electron spins associated with single defects in solids is a critical operation for a range of quantum information and measurement applications under development. So far, it has been accomplished for only two defect centres in crystalline solids: phosphorus dopants in silicon, for which electrical rea...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12081
更新日期:2013-05-02 00:00:00
abstract::Quantum computers are expected to outperform conventional computers in several important applications, from molecular simulation to search algorithms, once they can be scaled up to large numbers-typically millions-of quantum bits (qubits)1-3. For most solid-state qubit technologies-for example, those using superconduc...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2171-6
更新日期:2020-04-01 00:00:00
abstract::Galactic cosmic rays (CRs) are widely believed to be accelerated by shock waves associated with the expansion of supernova ejecta into the interstellar medium. A key issue in this long-standing conjecture is a theoretical prediction that the interstellar magnetic field can be substantially amplified at the shock of a ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature06210
更新日期:2007-10-04 00:00:00
abstract::Graphene oxide membranes-partially oxidized, stacked sheets of graphene-can provide ultrathin, high-flux and energy-efficient membranes for precise ionic and molecular sieving in aqueous solution. These materials have shown potential in a variety of applications, including water desalination and purification, gas and ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature24044
更新日期:2017-10-19 00:00:00