Abstract：
:Ribosomes and a subset of cellular mRNAs are trafficked into axons of developing neurons. The axonal localization of translational machinery allows new proteins to be rapidly and locally synthesized during axonal growth and pathfinding. However, in mature neurons, axonal ribosomes are significantly reduced or even absent. The mechanism that elicits this removal is currently unknown. Here, we demonstrate that synapse formation is the trigger for ribosome reduction in mature axons. In vivo analysis shows that axonal ribosome levels decrease in rat brain at a developmental stage coincident with synapse formation. Next, we observe in vitro that different synaptogenic inducers trigger an overall decrease of ribosomal proteins and rRNA in the axons of spinal motor neurons. We further observe that this process is dependent on the ubiquitin-proteasome system but not on autophagy. Together, these data identify synaptogenesis as the long missing biological trigger that leads to ribosome disappearance during axonal maturation.
journal_name
Cell Repjournal_title
Cell reportsauthors
Costa RO,Martins H,Martins LF,Cwetsch AW,Mele M,Pedro JR,Tomé D,Jeon NL,Cancedda L,Jaffrey SR,Almeida RDdoi
10.1016/j.celrep.2019.06.080subject
Has Abstractpub_date
2019-07-23 00:00:00pages
864-876.e6issue
4issn
2211-1247pii
S2211-1247(19)30860-5journal_volume
28pub_type
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