Abstract:
:It is known that plant cells can contain multiple distinct vacuoles; however, the abundance of multivacuolar cells and the mechanisms underlying vacuolar differentiation and communication among different types of vacuoles remain unknown. PH1 and PH5 are tonoplast P-ATPases that form a heteromeric pump that hyper-acidifies the central vacuole (CV) of epidermal cells in petunia petals. Here, we show that the sorting of this pump and other vacuolar proteins to the CV involves transit through small vacuoles: vacuolinos. Vacuolino formation is controlled by transcription factors regulating pigment synthesis and transcription of PH1 and PH5. Trafficking of proteins from vacuolinos to the central vacuole is impaired by misexpression of vacuolar SNAREs as well as mutants for the PH1 component of the PH1-PH5 pump. The finding that PH1-PH5 and these SNAREs interact strongly suggests that structural tonoplast proteins can act as tethering factors in the recognition of different vacuolar types.
journal_name
Cell Repjournal_title
Cell reportsauthors
Faraco M,Li Y,Li S,Spelt C,Di Sansebastiano GP,Reale L,Ferranti F,Verweij W,Koes R,Quattrocchio FMdoi
10.1016/j.celrep.2017.05.076subject
Has Abstractpub_date
2017-06-20 00:00:00pages
2413-2422issue
12issn
2211-1247pii
S2211-1247(17)30750-7journal_volume
19pub_type
杂志文章相关文献
Cell Reports文献大全abstract::At presynaptic terminals, neurotransmitters are released by synaptic vesicle exocytosis at the active zone. In order to maintain efficient neurotransmission and proper synaptic structure, sites of vesicle fusion must be cleared rapidly by endocytosis. Therefore, the coupling of exo- and endocytosis is crucial. Despite...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.11.060
更新日期:2019-12-17 00:00:00
abstract::Calcium signaling has long been associated with key events of immunity, including chemotaxis, phagocytosis, and activation. However, imaging and manipulation of calcium flux in motile immune cells in live animals remain challenging. Using light-sheet microscopy for in vivo calcium imaging in zebrafish, we observe char...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.11.010
更新日期:2015-12-15 00:00:00
abstract::One critical event in reprogramming to pluripotency is erasure of the somatic transcriptional program of starting cells. Here, we present the proof of principle of a strategy for reprogramming to pluripotency facilitated by small molecules that interfere with the somatic transcriptional memory. We show that mild chemi...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.08.060
更新日期:2016-09-20 00:00:00
abstract::SARM1 function and nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) loss both promote axon degeneration, but their relative relationship in the process is unknown. Here, we show that NMNAT2 loss and resultant changes to NMNAT metabolites occur in injured SARM1-deficient axons despite their delayed degenerati...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.02.060
更新日期:2015-03-31 00:00:00
abstract::Here, we ask how neural stem cells (NSCs) transition in the developing neocortex from a rapidly to a slowly proliferating state, a process required to maintain lifelong stem cell pools. We identify LRIG1, known to regulate receptor tyrosine kinase signaling in other cell types, as a negative regulator of cortical NSC ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108257
更新日期:2020-10-13 00:00:00
abstract::CELF6 is a CELF-RNA-binding protein, and thus part of a protein family with roles in human disease; however, its mRNA targets in the brain are largely unknown. Using cross-linking immunoprecipitation and sequencing (CLIP-seq), we define its CNS targets, which are enriched for 3' UTRs in synaptic protein-coding genes. ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108531
更新日期:2020-12-22 00:00:00
abstract::Experimental large-scale screens for drug repositioning are limited by restriction to in vitro conditions and lack of applicability to real human conditions. Here, we developed an in silico screen in human in vivo conditions using a reference of single gene mutations' non-tissue-specific "core transcriptome signatures...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.09.031
更新日期:2018-10-09 00:00:00
abstract::Alzheimer's disease (AD) is a neurodegenerative disease biochemically characterized by aberrant protein aggregation, including amyloid beta (Aβ) peptide accumulation. Protein aggregates in the cell are cleared by autophagy, a mechanism impaired in AD. To investigate the role of autophagy in Aβ pathology in vivo, we cr...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.08.042
更新日期:2013-10-17 00:00:00
abstract::Pre-implantation embryo development is an intricate and precisely regulated process orchestrated by maternally inherited proteins and newly synthesized proteins following zygotic genome activation. Although genomic and transcriptomic studies have enriched our understanding of the genetic programs underlying this proce...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.11.111
更新日期:2017-12-26 00:00:00
abstract::After implantation, pluripotent epiblasts are converted to embryonic ectoderm through cell-cell interactions that significantly change the transcriptional and epigenetic networks. An entrée to understanding this vital developmental transition is the t(w5) mutation of the mouse t complex. This mutation produces highly ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2012.10.004
更新日期:2012-11-29 00:00:00
abstract::In the presynaptic terminal, the magnitude and location of Ca2+ entry through voltage-gated Ca2+ channels (VGCCs) regulate the efficacy of neurotransmitter release. However, how presynaptic active zone proteins control mammalian VGCC levels and organization is unclear. To address this, we deleted the CAST/ELKS protein...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.06.024
更新日期:2018-07-10 00:00:00
abstract::Spinal cord longitudinal axons comprise some of the longest axons in our body. However, mechanisms that drive this extra long-distance axonal growth are largely unclear. We found that ascending axons of rapidly adapting (RA) mechanoreceptors closely abut a previously undescribed population of roof plate-derived radial...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.05.025
更新日期:2018-06-05 00:00:00
abstract::Sex differences in autoimmunity and infection suggest that a better understanding of molecular sex differences will improve the diagnosis and treatment of immune-related disease. We identified 144 differentially expressed genes, referred to as immune sex expression signature (iSEXS), between human males and females us...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.10.019
更新日期:2019-11-12 00:00:00
abstract::Information gained during goal pursuit motivates adaptive behavior. The anterior cingulate cortex (ACC) supports adaptive behavior, but how ACC signals are translated into motivational signals remains unclear. Rats with implants in the ACC and ventral tegmental area (VTA), a dopaminergic brain area implicated in motiv...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.05.062
更新日期:2017-06-13 00:00:00
abstract::Salivary proteins are essential for maintaining health in the oral cavity and proximal digestive tract, and they serve as potential diagnostic markers for monitoring human health and disease. However, their precise organ origins remain unclear. Through transcriptomic analysis of major adult and fetal salivary glands a...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108402
更新日期:2020-11-17 00:00:00
abstract::We report that the mammalian 5-methylcytosine (5mC) oxidase Tet3 exists as three major isoforms and characterized the full-length isoform containing an N-terminal CXXC domain (Tet3FL). This CXXC domain binds to unmethylated CpGs, but, unexpectedly, its highest affinity is toward 5-carboxylcytosine (5caC). We determine...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.12.044
更新日期:2016-01-26 00:00:00
abstract::Pneumonias caused by influenza A virus (IAV) co- and secondary bacterial infections are characterized by their severity and high mortality rate. Previously, we have shown that bacterial pore-forming toxin (PFT)-mediated necroptosis is a key driver of acute lung injury during bacterial pneumonia. Here, we evaluate the ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108062
更新日期:2020-08-25 00:00:00
abstract::The yeast protein Ipa1 was recently discovered to interact with the Ysh1 endonuclease of the pre-mRNA cleavage and polyadenylation (C/P) machinery, and Ipa1 mutation impairs 3'end processing. We report that Ipa1 globally promotes proper transcription termination and poly(A) site selection, but with variable effects on...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.01.051
更新日期:2019-02-12 00:00:00
abstract::Endosomal sorting complexes required for transport (ESCRT) drive cell surface receptor degradation resulting in attenuation of oncogenic signaling and pointing to a tumor suppressor function. Here, we show that loss of function of an ESCRT protein (HD-PTP encoded by the PTPN23 gene, located on the tumor suppressor gen...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.04.076
更新日期:2016-05-31 00:00:00
abstract::Signal transducer and activator of transcription 3 (STAT3) is a transcription factor central to axon regrowth with an enigmatic ability to act in different subcellular regions independently of its transcriptional roles. However, its roles in mature CNS neurons remain unclear. Here, we show that along with nuclear tran...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.03.029
更新日期:2016-04-12 00:00:00
abstract::Thymic epithelial cells (TECs) are critically required for T cell development, but the cellular mechanisms that maintain adult TECs are poorly understood. Here, we show that a previously unidentified subpopulation, EpCam(+)UEA1(-)Ly-51(+)PLET1(+)MHC class II(hi), which comprises <0.5% of adult TECs, contains bipotent ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.02.080
更新日期:2016-03-29 00:00:00
abstract::Embryonic stem cells (ESCs) hold promise for the treatment of many medical conditions; however, their utility is limited by immune rejection. The objective of our study is to establish tolerance or promote engraftment of transplanted ESCs as well as mature cell populations derived from ESCs. Luciferase (luc(+))-expres...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.02.050
更新日期:2015-03-24 00:00:00
abstract::Stable wakefulness requires orexin/hypocretin neurons (OHNs) and OHR2 receptors. OHNs sense diverse environmental cues and control arousal accordingly. For unknown reasons, OHNs contain multiple excitatory transmitters, including OH peptides and glutamate. To analyze their cotransmission within computational framework...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.03.055
更新日期:2014-05-08 00:00:00
abstract::Most human cancers originate from high-turnover tissues, while low-proliferating tissues, like skeletal muscle, exhibit a lower incidence of tumor development. In Duchenne muscular dystrophy (DMD), which induces increased skeletal muscle regeneration, tumor incidence is increased. Rhabdomyosarcomas (RMSs), a rare and ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.12.089
更新日期:2019-01-15 00:00:00
abstract::Health and survival in old age can be improved by changes in gene expression. RNA polymerase (Pol) I is the essential, conserved enzyme whose task is to generate the pre-ribosomal RNA (rRNA). We find that reducing the levels of Pol I activity is sufficient to extend lifespan in the fruit fly. This effect can be recapi...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.01.017
更新日期:2020-02-11 00:00:00
abstract::The mechanisms by which p53 suppresses tumor growth remain ill defined. In this issue of Cell Reports, Timofeev et al. (2013) and Valente et al. (2013) reveal context-dependent contributions of p53-dependent apoptosis to its tumor-suppressive function. ...
journal_title:Cell reports
pub_type: 评论,杂志文章
doi:10.1016/j.celrep.2013.05.011
更新日期:2013-05-30 00:00:00
abstract::Glioblastoma multiforme (GBM) is among the most aggressive of human cancers. Although differentiation therapy has been proposed as a potential approach to treat GBM, the mechanisms of induced differentiation remain poorly defined. Here, we established an induced differentiation model of GBM using cAMP activators that ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.12.037
更新日期:2017-01-10 00:00:00
abstract::We recently reported an autosomal dominant form of renal Fanconi syndrome caused by a missense mutation in the third codon of the peroxisomal protein EHHADH. The mutation mistargets EHHADH to mitochondria, thereby impairing mitochondrial energy production and, consequently, reabsorption of electrolytes and low-molecul...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.04.037
更新日期:2016-05-17 00:00:00
abstract::In mammalian skin, Merkel cells are mechanoreceptor cells that are required for the perception of gentle touch. Recent evidence indicates that mature Merkel cells descend from the proliferative layer of skin epidermis; however, the stem cell niche for Merkel cell homeostasis has not been reported. Here, we provide gen...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.04.026
更新日期:2013-06-27 00:00:00
abstract::We have previously found that UV irradiation promotes RNA polymerase II (RNAPII) hyperphosphorylation and subsequent changes in alternative splicing (AS). We show now that UV-induced DNA damage is not only necessary but sufficient to trigger the AS response and that photolyase-mediated removal of the most abundant cla...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.02.066
更新日期:2017-03-21 00:00:00