Abstract：
:Calcium signaling has long been associated with key events of immunity, including chemotaxis, phagocytosis, and activation. However, imaging and manipulation of calcium flux in motile immune cells in live animals remain challenging. Using light-sheet microscopy for in vivo calcium imaging in zebrafish, we observe characteristic patterns of calcium flux triggered by distinct events, including phagocytosis of pathogenic bacteria and migration of neutrophils toward inflammatory stimuli. In contrast to findings from ex vivo studies, we observe enriched calcium influx at the leading edge of migrating neutrophils. To directly manipulate calcium dynamics in vivo, we have developed transgenic lines with cell-specific expression of the mammalian TRPV1 channel, enabling ligand-gated, reversible, and spatiotemporal control of calcium influx. We find that controlled calcium influx can function to help define the neutrophil's leading edge. Cell-specific TRPV1 expression may have broad utility for precise control of calcium dynamics in other immune cell types and organisms.
journal_name
Cell Repjournal_title
Cell reportsauthors
Beerman RW,Matty MA,Au GG,Looger LL,Choudhury KR,Keller PJ,Tobin DMdoi
10.1016/j.celrep.2015.11.010subject
Has Abstractpub_date
2015-12-15 00:00:00pages
2107-17issue
10issn
2211-1247pii
S2211-1247(15)01310-8journal_volume
13pub_type
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