Abstract：
:Governance of protein phosphorylation by kinases and phosphatases constitutes an essential regulatory network in eukaryotic cells. Network dysregulation leads to severe consequences and is often a key factor in disease pathogenesis. Previous studies revealed multiple roles for protein phosphorylation and pathway structures in cellular functions from different perspectives. We seek to understand the roles of kinases and phosphatases from a protein homeostasis point of view. Using a streamlined tandem mass tag (SL-TMT) strategy, we systematically measure proteomic and phosphoproteomic responses to perturbations of phosphorylation signaling networks in yeast deletion strains. Our results emphasize the requirement for protein normalization for more complete interpretation of phosphorylation data. Functional relationships between kinases and phosphatases were characterized at both proteome and phosphoproteome levels in three ways: (1) Gene Ontology enrichment analysis, (2) Δgene-Δgene correlation networks, and (3) molecule covariance networks. This resource illuminates kinase and phosphatase functions and pathway organizations.
journal_name
Cell Repjournal_title
Cell reportsauthors
Li J,Paulo JA,Nusinow DP,Huttlin EL,Gygi SPdoi
10.1016/j.celrep.2019.10.034subject
Has Abstractpub_date
2019-11-12 00:00:00pages
2092-2104.e4issue
7issn
2211-1247pii
S2211-1247(19)31334-8journal_volume
29pub_type
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