Abstract：
:Two-photon imaging with genetically encoded calcium indicators (GECIs) enables long-term observation of neuronal activity in vivo. However, there are very few studies of GECIs in primates. Here, we report a method for long-term imaging of a GECI, GCaMP6f, expressed from adeno-associated virus vectors in cortical neurons of the adult common marmoset (Callithrix jacchus), a small New World primate. We used a tetracycline-inducible expression system to robustly amplify neuronal GCaMP6f expression and up- and downregulate it for more than 100 days. We succeeded in monitoring spontaneous activity not only from hundreds of neurons three-dimensionally distributed in layers 2 and 3 but also from single dendrites and axons in layer 1. Furthermore, we detected selective activities from somata, dendrites, and axons in the somatosensory cortex responding to specific tactile stimuli. Our results provide a way to investigate the organization and plasticity of cortical microcircuits at subcellular resolution in non-human primates.
journal_name
Cell Repjournal_title
Cell reportsauthors
Sadakane O,Masamizu Y,Watakabe A,Terada S,Ohtsuka M,Takaji M,Mizukami H,Ozawa K,Kawasaki H,Matsuzaki M,Yamamori Tdoi
10.1016/j.celrep.2015.10.050subject
Has Abstractpub_date
2015-12-01 00:00:00pages
1989-99issue
9issn
2211-1247pii
S2211-1247(15)01239-5journal_volume
13pub_type
杂志文章相关文献
Cell Reports文献大全abstract：:G9a is a lysine methyltransferase that regulates epigenetic modifications, transcription, and genome organization. However, whether these properties are dependent on one another or represent distinct functions of G9a remains unclear. In this study, we observe widespread DNA methylation loss in G9a depleted and catalyt...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108315
更新日期：2020-10-27 00:00:00
abstract：:Megakaryocytes (MKs) come from a complex process of hematopoietic progenitor maturation within the bone marrow that gives rise to de novo circulating platelets. Bone marrow microenvironment contains a large number of adipocytes with a still ill-defined role. This study aims to analyze the influence of adipocytes and i...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107875
更新日期：2020-07-07 00:00:00
abstract：:In many organs, stem cell function depends on communication with their niche partners. Cranial sensory neurons develop in close proximity to blood vessels; however, whether vasculature is an integral component of their niches is yet unknown. Here, two separate roles for vasculature in cranial sensory neurogenesis in z...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107903
更新日期：2020-07-14 00:00:00
abstract：:Cell size control emerges from a regulated balance between the rates of cell growth and division. In bacteria, simple quantitative laws connect cellular growth rate to ribosome abundance. However, it remains poorly understood how translation regulates bacterial cell size and shape under growth perturbations. Here, we ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108183
更新日期：2020-09-22 00:00:00
abstract：:Pancreatitis is an inflammatory disease of the exocrine pancreas and ranks among the most common gastrointestinal disorders. Inflamed tissues frequently experience conditions of insufficient oxygen availability, or hypoxia. Here, we demonstrate that hypoxia and consequent stabilization of the hypoxia-inducible factor ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.05.071
更新日期：2018-06-19 00:00:00
abstract：:Kainate receptors (KARs) mediate postsynaptic currents with a key impact on neuronal excitability. However, the molecular determinants controlling KAR postsynaptic localization and stabilization are poorly understood. Here, we exploit optogenetic and single-particle tracking approaches to study the role of KAR conform...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107735
更新日期：2020-06-09 00:00:00
abstract：:53BP1 plays a central role in dictating DNA repair choice between non-homologous end joining (NHEJ) and homologous recombination (HR), which is important for the sensitivity to poly(ADP-ribose) polymerase inhibitors (PARPis) of BRCA1-deficient cancers. In this study, we show that FOXK1 associates with 53BP1 and regula...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108018
更新日期：2020-08-11 00:00:00
abstract：:Transcriptional analysis of brain tissue from people with molecularly defined causes of obesity may highlight disease mechanisms and therapeutic targets. We performed RNA sequencing of hypothalamus from individuals with Prader-Willi syndrome (PWS), a genetic obesity syndrome characterized by severe hyperphagia. We fou...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.03.018
更新日期：2018-03-27 00:00:00
abstract：:Cell-cycle arrest in quiescence and senescence is largely orchestrated by the retinoblastoma (Rb) tumor-suppressor pathway, but the mechanisms underlying the quiescence-senescence switch remain unclear. Here, we show that the crosstalk between the Rb-AKT-signaling pathways forms this switch by controlling the overlapp...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.03.006
更新日期：2014-04-10 00:00:00
abstract：:How ubiquitous transcription factors (TFs) coordinate temporal inputs from broadly expressed epigenetic factors to control cell fate remains poorly understood. Here, we uncover a molecular relationship between p53, an abundant embryonic TF, and WDR5, an essential member of the MLL chromatin modifying complex, that reg...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.12.039
更新日期：2020-01-14 00:00:00
abstract：:Escape responses to threatening stimuli are vital for survival in all animal species. Larval zebrafish display fast escape responses when exposed to tactile, acoustic, and visual stimuli. However, their behavioral responses to chemosensory stimuli remain unknown. In this study, we found that carbon dioxide (CO2) induc...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.01.019
更新日期：2018-01-30 00:00:00
abstract：:Molecular and behavioral responses to opioids are thought to be primarily mediated by neurons, although there is accumulating evidence that other cell types play a prominent role in drug addiction. To investigate cell-type-specific opioid responses, we performed single-cell RNA sequencing (scRNA-seq) of the nucleus ac...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.08.080
更新日期：2018-09-25 00:00:00
abstract：:Synaptic efficacy plays crucial roles in neuronal circuit operation and synaptic plasticity. Presynaptic determinants of synaptic efficacy are neurotransmitter content in synaptic vesicles and the number of vesicles undergoing exocytosis at a time. Bursts of presynaptic firings depress synaptic efficacy, mainly due to...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.02.080
更新日期：2018-03-20 00:00:00
abstract：:Amphotericin, miconazole, and ciclopirox are antifungal agents from three different drug classes that can effectively kill planktonic yeast, yet their complete fungicidal mechanisms are not fully understood. Here, we employ a systems biology approach to identify a common oxidative-damage cellular death pathway trigger...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2012.12.021
更新日期：2013-02-21 00:00:00
abstract：:Viral persistence specifically inhibits CD4 Th1 responses and promotes Tfh immunity, but the mechanisms that suppress Th1 cells and the disease consequences of their loss are unclear. Here, we demonstrate that the loss of CD4 Th1 cells specifically leads to progressive CD8 T cell decline and dysfunction during viral p...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.08.065
更新日期：2016-09-20 00:00:00
abstract：:In neurons, transmembrane proteins are targeted to dendrites in vesicles that traffic solely within the somatodendritic compartment. How these vesicles are retained within the somatodendritic domain is unknown. Here, we use a novel pulse-chase system, which allows synchronous release of exogenous transmembrane protein...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2012.05.018
更新日期：2012-07-26 00:00:00
abstract：:The development of an embryo requires precise spatiotemporal regulation of cellular processes. During Drosophila gastrulation, a precise temporal pattern of cell division is encoded through transcriptional regulation of cdc25(string) in 25 distinct mitotic domains. Using a genetic screen, we demonstrate that the same ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.08.034
更新日期：2016-09-13 00:00:00
abstract：:The hormone αKlotho regulates lifespan in mice, as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast, the overexpression of αKlotho increases lifespan. Given the severe mouse phenotype, we generated zebrafish mutants for αklotho as well as i...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.08.013
更新日期：2019-09-10 00:00:00
abstract：:Eukaryotic DNA replication licensing is a prerequisite for, and plays a role in, regulating genome duplication that occurs exactly once per cell cycle. ORC (origin recognition complex) binds to and marks replication origins throughout the cell cycle and loads other replication-initiation proteins onto replication orig...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.02.046
更新日期：2020-03-10 00:00:00
abstract：:Working memory is mediated by the coordinated activation of frontal and parietal cortices occurring in the theta and alpha frequency ranges. Here, we test whether electrically stimulating frontal and parietal regions at the frequency of interaction is effective in modulating working memory. We identify working memory ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.10.072
更新日期：2019-11-26 00:00:00
abstract：:The subgranular zone (SGZ) of the adult hippocampus contains a pool of quiescent neural progenitor cells (QNPs) that are capable of entering the cell cycle and producing newborn neurons. The mechanisms that control the timing and extent of adult neurogenesis are not well understood. Here, we show that QNPs of the adul...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.10.037
更新日期：2013-11-27 00:00:00
abstract：:Adipose tissue fibrosis development blocks adipocyte hypertrophy and favors ectopic lipid accumulation. Here, we show that adipose tissue fibrosis is associated with obesity and insulin resistance in humans and mice. Kinetic studies in C3H mice fed a high-fat diet show activation of macrophages and progression of fibr...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.03.062
更新日期：2014-05-22 00:00:00
abstract：:Small cytoplasmic mRNP granules are implicated in mRNA transport, translational control, and decay. Using super-resolution microscopy and fluorescence correlation spectroscopy, we analyzed the molecular composition and dynamics of single cytoplasmic YBX1_IMP1 mRNP granules in live cells. Granules appeared elongated an...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.09.018
更新日期：2019-10-15 00:00:00
abstract：:The INO80 complex (INO80-C) is an evolutionarily conserved nucleosome remodeler that acts in transcription, replication, and genome stability. It is required for resistance against genotoxic agents and is involved in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR). However, the causes of...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.04.051
更新日期：2017-05-16 00:00:00
abstract：:Eukaryotic genomes are almost entirely transcribed by RNA polymerase II. Consequently, the transcription of long noncoding RNAs often overlaps with coding gene promoters, triggering potential gene repression through a poorly characterized mechanism of transcription interference. Here, we propose a comprehensive model ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107612
更新日期：2020-05-05 00:00:00
abstract：:Premature closure of the sutures that connect the cranial bones during development of the mammalian skull results in a phenotype called craniosynostosis. Recently, several craniosynostosis patients with missense mutations within the gene encoding the interleukin-11 receptor (IL-11R) have been described, but the underl...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.09.005
更新日期：2018-10-02 00:00:00
abstract：:CD4+ Foxp3+ T regulatory (Treg) cells are key players in preventing lethal autoimmunity. Tregs undertake differentiation processes and acquire diverse functional properties. However, how Treg's differentiation and functional specification are regulated remains incompletely understood. Here, we report that gradient exp...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.05.061
更新日期：2019-06-18 00:00:00
abstract：:Complex febrile seizures (FSs) lead to a high risk of intractable temporal lobe epilepsy during adulthood, yet the pathological process of complex FSs is largely unknown. Here, we demonstrate that activated microglia extensively associated with glutamatergic neuronal soma displace surrounding GABAergic presynapses in ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108346
更新日期：2020-11-03 00:00:00
abstract：:Cell growth is coupled to cell-cycle progression in mitotically proliferating mammalian cells, but the underlying molecular mechanisms are not well understood. CyclinD-Cdk4/6 is known to phosphorylate RB to promote S-phase entry, but recent work suggests they have additional functions. We show here that CyclinD-Cdk4/6...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.03.068
更新日期：2020-04-14 00:00:00
abstract：:Kinase inhibitors represent the backbone of targeted cancer therapy, yet only a limited number of oncogenic drivers are directly druggable. By interrogating the activity of 1,505 kinase inhibitors, we found that BRD4-NUT-rearranged NUT midline carcinoma (NMC) cells are specifically killed by CDK9 inhibition (CDK9i) an...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.08.082
更新日期：2017-09-19 00:00:00
