Single-Cell RNA-Seq Uncovers a Robust Transcriptional Response to Morphine by Glia.


:Molecular and behavioral responses to opioids are thought to be primarily mediated by neurons, although there is accumulating evidence that other cell types play a prominent role in drug addiction. To investigate cell-type-specific opioid responses, we performed single-cell RNA sequencing (scRNA-seq) of the nucleus accumbens of mice following acute morphine treatment. Differential expression analysis uncovered unique morphine-dependent transcriptional responses by oligodendrocytes and astrocytes. We examined the expression of selected genes, including Cdkn1a and Sgk1, by FISH, confirming their induction by morphine in oligodendrocytes. Further analysis using RNA-seq of FACS-purified oligodendrocytes revealed a large cohort of morphine-regulated genes. The affected genes are enriched for roles in cellular pathways intimately linked to oligodendrocyte maturation and myelination, including the unfolded protein response. Altogether, our data illuminate the morphine-dependent transcriptional response by oligodendrocytes and offer mechanistic insights into myelination defects associated with opioid abuse.


Cell Rep


Cell reports


Avey D,Sankararaman S,Yim AKY,Barve R,Milbrandt J,Mitra RD




Has Abstract


2018-09-25 00:00:00












  • Drosophila Vision Depends on Carcinine Uptake by an Organic Cation Transporter.

    abstract::Recycling of neurotransmitters is essential for sustained neuronal signaling, yet recycling pathways for various transmitters, including histamine, remain poorly understood. In the first visual ganglion (lamina) of Drosophila, photoreceptor-released histamine is taken up into perisynaptic glia, converted to carcinine,...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Chaturvedi R,Luan Z,Guo P,Li HS

    更新日期:2016-03-08 00:00:00

  • Presenilin 2 Modulates Endoplasmic Reticulum-Mitochondria Coupling by Tuning the Antagonistic Effect of Mitofusin 2.

    abstract::Communication between organelles plays key roles in cell biology. In particular, physical and functional coupling of the endoplasmic reticulum (ER) and mitochondria is crucial for regulation of various physiological and pathophysiological processes. Here, we demonstrate that Presenilin 2 (PS2), mutations in which unde...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Filadi R,Greotti E,Turacchio G,Luini A,Pozzan T,Pizzo P

    更新日期:2016-06-07 00:00:00

  • Theta Phase-Coordinated Memory Reactivation Reoccurs in a Slow-Oscillatory Rhythm during NREM Sleep.

    abstract::It has been proposed that sleep's contribution to memory consolidation is to reactivate prior encoded information. To elucidate the neural mechanisms carrying reactivation-related mnemonic information, we investigated whether content-specific memory signatures associated with memory reactivation during wakefulness reo...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Schreiner T,Doeller CF,Jensen O,Rasch B,Staudigl T

    更新日期:2018-10-09 00:00:00

  • Piwi Is a Key Regulator of Both Somatic and Germline Stem Cells in the Drosophila Testis.

    abstract::The Piwi-piRNA pathway is well known for its germline function, yet its somatic role remains elusive. We show here that Piwi is required autonomously not only for germline stem cell (GSC) but also for somatic cyst stem cell (CySC) maintenance in the Drosophila testis. Reducing Piwi activity in the testis caused defect...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Gonzalez J,Qi H,Liu N,Lin H

    更新日期:2015-07-07 00:00:00

  • Insights into Centromere DNA Bending Revealed by the Cryo-EM Structure of the Core Centromere Binding Factor 3 with Ndc10.

    abstract::The centromere binding factor 3 (CBF3) complex binds the third centromere DNA element in organisms with point centromeres, such as S. cerevisiae. It is an essential complex for assembly of the kinetochore in these organisms, as it facilitates genetic centromere specification and allows association of all other kinetoc...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Zhang W,Lukoyanova N,Miah S,Lucas J,Vaughan CK

    更新日期:2018-07-17 00:00:00

  • A Mechanism Linking Two Known Vulnerability Factors for Alcohol Abuse: Heightened Alcohol Stimulation and Low Striatal Dopamine D2 Receptors.

    abstract::Alcohol produces both stimulant and sedative effects in humans and rodents. In humans, alcohol abuse disorder is associated with a higher stimulant and lower sedative responses to alcohol. Here, we show that this association is conserved in mice and demonstrate a causal link with another liability factor: low expressi...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Bocarsly ME,da Silva E Silva D,Kolb V,Luderman KD,Shashikiran S,Rubinstein M,Sibley DR,Dobbs LK,Alvarez VA

    更新日期:2019-10-29 00:00:00

  • Fusion of Reprogramming Factors Alters the Trajectory of Somatic Lineage Conversion.

    abstract::Simultaneous expression of Oct4, Klf4, Sox2, and cMyc induces pluripotency in somatic cells (iPSCs). Replacing Oct4 with the neuro-specific factor Brn4 leads to transdifferentiation of fibroblasts into induced neural stem cells (iNSCs). However, Brn4 was recently found to induce transient acquisition of pluripotency b...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Velychko S,Kang K,Kim SM,Kwak TH,Kim KP,Park C,Hong K,Chung C,Hyun JK,MacCarthy CM,Wu G,Schöler HR,Han DW

    更新日期:2019-04-02 00:00:00

  • Post-translational Modifications of OLIG2 Regulate Glioma Invasion through the TGF-β Pathway.

    abstract::In glioblastoma, invasion and proliferation are presumed to be mutually exclusive events; however, the molecular mechanisms that mediate this switch at the cellular level remain elusive. Previously, we have shown that phospho-OLIG2, a central-nervous-system-specific transcription factor, is essential for tumor growth ...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Singh SK,Fiorelli R,Kupp R,Rajan S,Szeto E,Lo Cascio C,Maire CL,Sun Y,Alberta JA,Eschbacher JM,Ligon KL,Berens ME,Sanai N,Mehta S

    更新日期:2016-07-26 00:00:00

  • XBP1-KLF9 Axis Acts as a Molecular Rheostat to Control the Transition from Adaptive to Cytotoxic Unfolded Protein Response.

    abstract::Transcription factor XBP1s, activated by endoplasmic reticulum (ER) stress in a dose-dependent manner, plays a central role in adaptive unfolded protein response (UPR) via direct activation of multiple genes controlling protein refolding. Here, we report that elevation of ER stress above a critical threshold causes ac...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Fink EE,Moparthy S,Bagati A,Bianchi-Smiraglia A,Lipchick BC,Wolff DW,Roll MV,Wang J,Liu S,Bakin AV,Kandel ES,Lee AH,Nikiforov MA

    更新日期:2018-10-02 00:00:00

  • Structural Insights into the Unique Activation Mechanisms of a Non-classical Calpain and Its Disease-Causing Variants.

    abstract::Increased calpain activity is linked to neuroinflammation including a heritable retinal disease caused by hyper-activating mutations in the calcium-activated calpain-5 (CAPN5) protease. Although structures for classical calpains are known, the structure of CAPN5, a non-classical calpain, remains undetermined. Here we ...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Velez G,Sun YJ,Khan S,Yang J,Herrmann J,Chemudupati T,MacLaren RE,Gakhar L,Wakatsuki S,Bassuk AG,Mahajan VB

    更新日期:2020-01-21 00:00:00

  • Hepatic Leukemia Factor Maintains Quiescence of Hematopoietic Stem Cells and Protects the Stem Cell Pool during Regeneration.

    abstract::The transcription factor hepatic leukemia factor (HLF) is strongly expressed in hematopoietic stem cells (HSCs) and is thought to influence both HSC self-renewal and leukemogenesis. However, the physiological role of HLF in hematopoiesis and HSC function is unclear. Here, we report that mice lacking Hlf are viable wit...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Komorowska K,Doyle A,Wahlestedt M,Subramaniam A,Debnath S,Chen J,Soneji S,Van Handel B,Mikkola HKA,Miharada K,Bryder D,Larsson J,Magnusson M

    更新日期:2017-12-19 00:00:00

  • Mpn1, mutated in poikiloderma with neutropenia protein 1, is a conserved 3'-to-5' RNA exonuclease processing U6 small nuclear RNA.

    abstract::Clericuzio-type poikiloderma with neutropenia (PN) is a rare genodermatosis associated with mutations in the C16orf57 gene, which codes for the uncharacterized protein hMpn1. We show here that, in both fission yeasts and humans, Mpn1 processes the spliceosomal U6 small nuclear RNA (snRNA) posttranscriptionally. In Mpn...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Shchepachev V,Wischnewski H,Missiaglia E,Soneson C,Azzalin CM

    更新日期:2012-10-25 00:00:00

  • Comparative Gene Expression Analyses Reveal Distinct Molecular Signatures between Differentially Reprogrammed Cardiomyocytes.

    abstract::Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) or directly reprogrammed from non-myocytes (induced cardiomyocytes [iCMs]) are promising sources for heart regeneration or disease modeling. However, the similarities and differences between iPSC-CMs and iCMs are still unknown. Here, we performed tr...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Zhou Y,Wang L,Liu Z,Alimohamadi S,Yin C,Liu J,Qian L

    更新日期:2017-09-26 00:00:00

  • Agrin as a Mechanotransduction Signal Regulating YAP through the Hippo Pathway.

    abstract::The Hippo pathway effectors YAP and TAZ act as nuclear sensors of mechanical signals in response to extracellular matrix (ECM) cues. However, the identity and nature of regulators in the ECM and the precise pathways relaying mechanoresponsive signals into intracellular sensors remain unclear. Here, we uncover a functi...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Chakraborty S,Njah K,Pobbati AV,Lim YB,Raju A,Lakshmanan M,Tergaonkar V,Lim CT,Hong W

    更新日期:2017-03-07 00:00:00

  • Mutual Zonated Interactions of Wnt and Hh Signaling Are Orchestrating the Metabolism of the Adult Liver in Mice and Human.

    abstract::The Hedgehog (Hh) and Wnt/β-Catenin (Wnt) cascades are morphogen pathways whose pronounced influence on adult liver metabolism has been identified in recent years. How both pathways communicate and control liver metabolic functions are largely unknown. Detecting core components of Wnt and Hh signaling and mathematical...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Kolbe E,Aleithe S,Rennert C,Spormann L,Ott F,Meierhofer D,Gajowski R,Stöpel C,Hoehme S,Kücken M,Brusch L,Seifert M,von Schoenfels W,Schafmayer C,Brosch M,Hofmann U,Damm G,Seehofer D,Hampe J,Gebhardt R,Matz-Soja M

    更新日期:2019-12-24 00:00:00

  • Neuronal Regulation of Schwann Cell Mitochondrial Ca(2+) Signaling during Myelination.

    abstract::Schwann cells (SCs) myelinate peripheral neurons to promote the rapid conduction of action potentials, and the process of myelination is known to be regulated by signals from axons to SCs. Given that SC mitochondria are one of the potential regulators of myelination, we investigated whether SC mitochondria are regulat...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Ino D,Sagara H,Suzuki J,Kanemaru K,Okubo Y,Iino M

    更新日期:2015-09-29 00:00:00

  • A Single Templating RNA in Yeast Telomerase.

    abstract::The number of essential telomerase components in the active ribonucleoprotein (RNP) has important implications for its mechanism of action yet is by and large unknown. We report that two differentially tagged TLC1 RNAs endogenously expressed in a heterozygous diploid and simultaneously detected via multi-color fluores...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Bajon E,Laterreur N,Wellinger RJ

    更新日期:2015-07-21 00:00:00

  • The Dachsous/Fat/Four-Jointed Pathway Directs the Uniform Axial Orientation of Epithelial Cells in the Drosophila Abdomen.

    abstract::The achievement of the final form of an individual requires not only the control of cell size and differentiation but also integrative directional cues to instruct cell movements, positions, and orientations. In Drosophila, the adult epidermis of the abdomen is created de novo by histoblasts. As these expand and fuse,...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Mangione F,Martín-Blanco E

    更新日期:2018-12-04 00:00:00

  • The Release of Adipose Stromal Cells from Subcutaneous Adipose Tissue Regulates Ectopic Intramuscular Adipocyte Deposition.

    abstract::Ectopic lipid deposition (ELD) is defined by excess fat storage in locations not classically associated with adipose tissue (AT) storage. ELD is positively correlated with insulin resistance and increased risk of metabolic disorders. ELD appears as lipid droplets or adipocytes, whose cell origin is unknown. We previou...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Girousse A,Gil-Ortega M,Bourlier V,Bergeaud C,Sastourné-Arrey Q,Moro C,Barreau C,Guissard C,Vion J,Arnaud E,Pradère JP,Juin N,Casteilla L,Sengenès C

    更新日期:2019-04-09 00:00:00

  • TLR4-Mediated Pathway Triggers Interferon-Independent G0 Arrest and Antiviral SAMHD1 Activity in Macrophages.

    abstract::Macrophages exist predominantly in two distinct states, G0 and a G1-like state that is accompanied by phosphorylation of SAMHD1 at T592. Here, we demonstrate that Toll-like receptor 4 (TLR4) activation can potently induce G0 arrest and SAMHD1 antiretroviral activity by an interferon (IFN)-independent pathway. This pat...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Mlcochova P,Winstone H,Zuliani-Alvarez L,Gupta RK

    更新日期:2020-03-24 00:00:00

  • Developmental Origin of Oligodendrocyte Lineage Cells Determines Response to Demyelination and Susceptibility to Age-Associated Functional Decline.

    abstract::Oligodendrocyte progenitors (OPs) arise from distinct ventral and dorsal domains within the ventricular germinal zones of the embryonic CNS. The functional significance, if any, of these different populations is not known. Using dual-color reporter mice to distinguish ventrally and dorsally derived OPs, we show that, ...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Crawford AH,Tripathi RB,Richardson WD,Franklin RJM

    更新日期:2016-04-26 00:00:00

  • The number of fetal nephron progenitor cells limits ureteric branching and adult nephron endowment.

    abstract::Nephrons, the functional units of the kidney, develop from progenitor cells (cap mesenchyme [CM]) surrounding the epithelial ureteric bud (UB) tips. Reciprocal signaling between UB and CM induces nephrogenesis and UB branching. Although low nephron number is implicated in hypertension and renal disease, the mechanisms...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Cebrian C,Asai N,D'Agati V,Costantini F

    更新日期:2014-04-10 00:00:00

  • Transcription factor networks in Drosophila melanogaster.

    abstract::Specific cellular fates and functions depend on differential gene expression, which occurs primarily at the transcriptional level and is controlled by complex regulatory networks of transcription factors (TFs). TFs act through combinatorial interactions with other TFs, cofactors, and chromatin-remodeling proteins. Her...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Rhee DY,Cho DY,Zhai B,Slattery M,Ma L,Mintseris J,Wong CY,White KP,Celniker SE,Przytycka TM,Gygi SP,Obar RA,Artavanis-Tsakonas S

    更新日期:2014-09-25 00:00:00

  • Degradation of Bcl-2 by XIAP and ARTS Promotes Apoptosis.

    abstract::We describe a mechanism by which the anti-apoptotic B cell lymphoma 2 (Bcl-2) protein is downregulated to induce apoptosis. ARTS (Sept4_i2) is a tumor suppressor protein that promotes cell death through specifically antagonizing XIAP (X-linked inhibitor of apoptosis). ARTS and Bcl-2 reside at the outer mitochondrial m...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Edison N,Curtz Y,Paland N,Mamriev D,Chorubczyk N,Haviv-Reingewertz T,Kfir N,Morgenstern D,Kupervaser M,Kagan J,Kim HT,Larisch S

    更新日期:2017-10-10 00:00:00

  • Identifying network motifs that buffer front-to-back signaling in polarized neutrophils.

    abstract::Neutrophil polarity relies on local, mutual inhibition to segregate incompatible signaling circuits to the leading and trailing edges. Mutual inhibition alone should lead to cells having strong fronts and weak backs or vice versa. However, analysis of cell-to-cell variation in human neutrophils revealed that back pola...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Wang Y,Ku CJ,Zhang ER,Artyukhin AB,Weiner OD,Wu LF,Altschuler SJ

    更新日期:2013-05-30 00:00:00

  • Symmetry Breaking in an Edgeless Epithelium by Fat2-Regulated Microtubule Polarity.

    abstract::Planar cell polarity (PCP) information is a critical determinant of organ morphogenesis. While PCP in bounded epithelial sheets is increasingly well understood, how PCP is organized in tubular and acinar tissues is not. Drosophila egg chambers (follicles) are an acinus-like "edgeless epithelium" and exhibit a continuo...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Chen DY,Lipari KR,Dehghan Y,Streichan SJ,Bilder D

    更新日期:2016-05-10 00:00:00

  • RNASEK Is a V-ATPase-Associated Factor Required for Endocytosis and the Replication of Rhinovirus, Influenza A Virus, and Dengue Virus.

    abstract::Human rhinovirus (HRV) causes upper respiratory infections and asthma exacerbations. We screened multiple orthologous RNAi reagents and identified host proteins that modulate HRV replication. Here, we show that RNASEK, a transmembrane protein, was needed for the replication of HRV, influenza A virus, and dengue virus....

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Perreira JM,Aker AM,Savidis G,Chin CR,McDougall WM,Portmann JM,Meraner P,Smith MC,Rahman M,Baker RE,Gauthier A,Franti M,Brass AL

    更新日期:2015-08-04 00:00:00

  • Continuously growing rodent molars result from a predictable quantitative evolutionary change over 50 million years.

    abstract::The fossil record is widely informative about evolution, but fossils are not systematically used to study the evolution of stem-cell-driven renewal. Here, we examined evolution of the continuous growth (hypselodonty) of rodent molar teeth, which is fuelled by the presence of dental stem cells. We studied occurrences o...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Tapaltsyan V,Eronen JT,Lawing AM,Sharir A,Janis C,Jernvall J,Klein OD

    更新日期:2015-05-05 00:00:00

  • Compromised Survival of Cerebellar Molecular Layer Interneurons Lacking GDNF Receptors GFRα1 or RET Impairs Normal Cerebellar Motor Learning.

    abstract::The role of neurotrophic factors as endogenous survival proteins for brain neurons remains contentious. In the cerebellum, the signals controlling survival of molecular layer interneurons (MLIs) are unknown, and direct evidence for the requirement of a full complement of MLIs for normal cerebellar function and motor l...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Sergaki MC,López-Ramos JC,Stagkourakis S,Gruart A,Broberger C,Delgado-García JM,Ibáñez CF

    更新日期:2017-06-06 00:00:00

  • CFP1 Regulates Histone H3K4 Trimethylation and Developmental Potential in Mouse Oocytes.

    abstract::Trimethylation of histone H3 at lysine-4 (H3K4me3) is associated with eukaryotic gene promoters and poises their transcriptional activation during development. To examine the in vivo function of H3K4me3 in the absence of DNA replication, we deleted CXXC finger protein 1 (CFP1), the DNA-binding subunit of the SETD1 his...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Yu C,Fan X,Sha QQ,Wang HH,Li BT,Dai XX,Shen L,Liu J,Wang L,Liu K,Tang F,Fan HY

    更新日期:2017-08-01 00:00:00