Abstract:
:Molecular and behavioral responses to opioids are thought to be primarily mediated by neurons, although there is accumulating evidence that other cell types play a prominent role in drug addiction. To investigate cell-type-specific opioid responses, we performed single-cell RNA sequencing (scRNA-seq) of the nucleus accumbens of mice following acute morphine treatment. Differential expression analysis uncovered unique morphine-dependent transcriptional responses by oligodendrocytes and astrocytes. We examined the expression of selected genes, including Cdkn1a and Sgk1, by FISH, confirming their induction by morphine in oligodendrocytes. Further analysis using RNA-seq of FACS-purified oligodendrocytes revealed a large cohort of morphine-regulated genes. The affected genes are enriched for roles in cellular pathways intimately linked to oligodendrocyte maturation and myelination, including the unfolded protein response. Altogether, our data illuminate the morphine-dependent transcriptional response by oligodendrocytes and offer mechanistic insights into myelination defects associated with opioid abuse.
journal_name
Cell Repjournal_title
Cell reportsauthors
Avey D,Sankararaman S,Yim AKY,Barve R,Milbrandt J,Mitra RDdoi
10.1016/j.celrep.2018.08.080subject
Has Abstractpub_date
2018-09-25 00:00:00pages
3619-3629.e4issue
13issn
2211-1247pii
S2211-1247(18)31384-6journal_volume
24pub_type
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