Single-Cell RNA-Seq Uncovers a Robust Transcriptional Response to Morphine by Glia.


:Molecular and behavioral responses to opioids are thought to be primarily mediated by neurons, although there is accumulating evidence that other cell types play a prominent role in drug addiction. To investigate cell-type-specific opioid responses, we performed single-cell RNA sequencing (scRNA-seq) of the nucleus accumbens of mice following acute morphine treatment. Differential expression analysis uncovered unique morphine-dependent transcriptional responses by oligodendrocytes and astrocytes. We examined the expression of selected genes, including Cdkn1a and Sgk1, by FISH, confirming their induction by morphine in oligodendrocytes. Further analysis using RNA-seq of FACS-purified oligodendrocytes revealed a large cohort of morphine-regulated genes. The affected genes are enriched for roles in cellular pathways intimately linked to oligodendrocyte maturation and myelination, including the unfolded protein response. Altogether, our data illuminate the morphine-dependent transcriptional response by oligodendrocytes and offer mechanistic insights into myelination defects associated with opioid abuse.


Cell Rep


Cell reports


Avey D,Sankararaman S,Yim AKY,Barve R,Milbrandt J,Mitra RD




Has Abstract


2018-09-25 00:00:00












  • The touch dome defines an epidermal niche specialized for mechanosensory signaling.

    abstract::In mammalian skin, Merkel cells are mechanoreceptor cells that are required for the perception of gentle touch. Recent evidence indicates that mature Merkel cells descend from the proliferative layer of skin epidermis; however, the stem cell niche for Merkel cell homeostasis has not been reported. Here, we provide gen...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Doucet YS,Woo SH,Ruiz ME,Owens DM

    更新日期:2013-06-27 00:00:00

  • CDK8 Regulates Insulin Secretion and Mediates Postnatal and Stress-Induced Expression of Neuropeptides in Pancreatic β Cells.

    abstract::Cyclin-dependent kinases (CDKs) contribute to vital cellular processes including cell cycle regulation. Loss of CDKs is associated with impaired insulin secretion and β cell survival; however, the function of CDK8 in β cells remains elusive. Here, we report that genetic ablation of Cdk8 improves glucose tolerance by i...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Xue J,Scotti E,Stoffel M

    更新日期:2019-09-10 00:00:00

  • Molecular Basis of Zinc-Dependent Endocytosis of Human ZIP4 Transceptor.

    abstract::Nutrient transporters can be rapidly removed from the cell surface via substrate-stimulated endocytosis as a way to control nutrient influx, but the molecular underpinnings are not well understood. In this work, we focus on zinc-dependent endocytosis of human ZIP4 (hZIP4), a zinc transporter that is essential for diet...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Zhang C,Sui D,Zhang T,Hu J

    更新日期:2020-04-28 00:00:00

  • Genetic Single Neuron Anatomy Reveals Fine Granularity of Cortical Axo-Axonic Cells.

    abstract::Parsing diverse nerve cells into biological types is necessary for understanding neural circuit organization. Morphology is an intuitive criterion for neuronal classification and a proxy of connectivity, but morphological diversity and variability often preclude resolving the granularity of neuron types. Combining gen...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Wang X,Tucciarone J,Jiang S,Yin F,Wang BS,Wang D,Jia Y,Jia X,Li Y,Yang T,Xu Z,Akram MA,Wang Y,Zeng S,Ascoli GA,Mitra P,Gong H,Luo Q,Huang ZJ

    更新日期:2019-03-12 00:00:00

  • The Release of Adipose Stromal Cells from Subcutaneous Adipose Tissue Regulates Ectopic Intramuscular Adipocyte Deposition.

    abstract::Ectopic lipid deposition (ELD) is defined by excess fat storage in locations not classically associated with adipose tissue (AT) storage. ELD is positively correlated with insulin resistance and increased risk of metabolic disorders. ELD appears as lipid droplets or adipocytes, whose cell origin is unknown. We previou...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Girousse A,Gil-Ortega M,Bourlier V,Bergeaud C,Sastourné-Arrey Q,Moro C,Barreau C,Guissard C,Vion J,Arnaud E,Pradère JP,Juin N,Casteilla L,Sengenès C

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  • Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of Treg Cells.

    abstract::The function of regulatory T (Treg) cells depends on lipid oxidation. However, the molecular mechanism by which Treg cells maintain lipid metabolism after activation remains elusive. Liver kinase B1 (LKB1) acts as a coordinator by linking cellular metabolism to substrate AMP-activated protein kinase (AMPK). We show th...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Timilshina M,You Z,Lacher SM,Acharya S,Jiang L,Kang Y,Kim JA,Chang HW,Kim KJ,Park B,Song JH,Ko HJ,Park YY,Ma MJ,Nepal MR,Jeong TC,Chung Y,Waisman A,Chang JH

    更新日期:2019-06-04 00:00:00

  • Single-Cell Transcriptomics of Traced Epidermal and Hair Follicle Stem Cells Reveals Rapid Adaptations during Wound Healing.

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Joost S,Jacob T,Sun X,Annusver K,La Manno G,Sur I,Kasper M

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  • MERVL/Zscan4 Network Activation Results in Transient Genome-wide DNA Demethylation of mESCs.

    abstract::Mouse embryonic stem cells are dynamic and heterogeneous. For example, rare cells cycle through a state characterized by decondensed chromatin and expression of transcripts, including the Zscan4 cluster and MERVL endogenous retrovirus, which are usually restricted to preimplantation embryos. Here, we further character...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Eckersley-Maslin MA,Svensson V,Krueger C,Stubbs TM,Giehr P,Krueger F,Miragaia RJ,Kyriakopoulos C,Berrens RV,Milagre I,Walter J,Teichmann SA,Reik W

    更新日期:2016-09-27 00:00:00

  • Renal Fanconi Syndrome Is Caused by a Mistargeting-Based Mitochondriopathy.

    abstract::We recently reported an autosomal dominant form of renal Fanconi syndrome caused by a missense mutation in the third codon of the peroxisomal protein EHHADH. The mutation mistargets EHHADH to mitochondria, thereby impairing mitochondrial energy production and, consequently, reabsorption of electrolytes and low-molecul...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Assmann N,Dettmer K,Simbuerger JMB,Broeker C,Nuernberger N,Renner K,Courtneidge H,Klootwijk ED,Duerkop A,Hall A,Kleta R,Oefner PJ,Reichold M,Reinders J

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  • Microglia modulate wiring of the embryonic forebrain.

    abstract::Dysfunction of microglia, the tissue macrophages of the brain, has been associated with the etiology of several neuropsychiatric disorders. Consistently, microglia have been shown to regulate neurogenesis and synaptic maturation at perinatal and postnatal stages. However, microglia invade the brain during mid-embryoge...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Squarzoni P,Oller G,Hoeffel G,Pont-Lezica L,Rostaing P,Low D,Bessis A,Ginhoux F,Garel S

    更新日期:2014-09-11 00:00:00

  • Cytoplasmic Control of Sense-Antisense mRNA Pairs.

    abstract::Transcriptome analyses have revealed that convergent gene transcription can produce many 3'-overlapping mRNAs in diverse organisms. Few studies have examined the fate of 3'-complementary mRNAs in double-stranded RNA-dependent nuclear phenomena, and nothing is known about the cytoplasmic destiny of 3'-overlapping messe...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Sinturel F,Navickas A,Wery M,Descrimes M,Morillon A,Torchet C,Benard L

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  • Wnd/DLK Is a Critical Target of FMRP Responsible for Neurodevelopmental and Behavior Defects in the Drosophila Model of Fragile X Syndrome.

    abstract::Fragile X syndrome (FXS) is the leading heritable cause of intellectual disability and commonly co-occurs with autism spectrum disorder. Silencing of the Fmr1 gene leads to the absence of the protein product, fragile X mental retardation protein (FMRP), which represses translation of many target mRNAs. Excess translat...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Russo A,DiAntonio A

    更新日期:2019-09-03 00:00:00

  • Downregulation of a GPCR by β-Arrestin2-Mediated Switch from an Endosomal to a TGN Recycling Pathway.

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Abdullah N,Beg M,Soares D,Dittman JS,McGraw TE

    更新日期:2016-12-13 00:00:00

  • miR-15/16 Restrain Memory T Cell Differentiation, Cell Cycle, and Survival.

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    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Gagnon JD,Kageyama R,Shehata HM,Fassett MS,Mar DJ,Wigton EJ,Johansson K,Litterman AJ,Odorizzi P,Simeonov D,Laidlaw BJ,Panduro M,Patel S,Jeker LT,Feeney ME,McManus MT,Marson A,Matloubian M,Sanjabi S,Ansel KM

    更新日期:2019-08-20 00:00:00

  • Fungicidal drugs induce a common oxidative-damage cellular death pathway.

    abstract::Amphotericin, miconazole, and ciclopirox are antifungal agents from three different drug classes that can effectively kill planktonic yeast, yet their complete fungicidal mechanisms are not fully understood. Here, we employ a systems biology approach to identify a common oxidative-damage cellular death pathway trigger...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Belenky P,Camacho D,Collins JJ

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  • MicroRNA Regulation of nAChR Expression and Nicotine-Dependent Behavior in C. elegans.

    abstract::Chronic exposure to nicotine upregulates nicotinic acetylcholine receptors (nAChRs), and such upregulation is critical for the development of nicotine dependence in humans and animal models. However, how nicotine upregulates nAChRs is not well understood. Here, we identify a key role for microRNA in regulating nicotin...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Rauthan M,Gong J,Liu J,Li Z,Wescott SA,Liu J,Xu XZS

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  • Discovery and Characterization of piRNAs in the Human Fetal Ovary.

    abstract::Piwi-interacting RNAs (piRNAs), a class of 26- to 32-nt non-coding RNAs (ncRNAs), function in germline development, transposon silencing, and epigenetic regulation. We performed deep sequencing and annotation of untreated and periodate-treated small RNA cDNA libraries from human fetal and adult germline and reference ...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Williams Z,Morozov P,Mihailovic A,Lin C,Puvvula PK,Juranek S,Rosenwaks Z,Tuschl T

    更新日期:2015-10-27 00:00:00

  • EBI2 Is Highly Expressed in Multiple Sclerosis Lesions and Promotes Early CNS Migration of Encephalitogenic CD4 T Cells.

    abstract::Arrival of encephalitogenic T cells at inflammatory foci represents a critical step in development of experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis. EBI2 and its ligand, 7α,25-OHC, direct immune cell localization in secondary lymphoid organs. CH25H and CYP7B1 hydroxylate chol...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Wanke F,Moos S,Croxford AL,Heinen AP,Gräf S,Kalt B,Tischner D,Zhang J,Christen I,Bruttger J,Yogev N,Tang Y,Zayoud M,Israel N,Karram K,Reißig S,Lacher SM,Reichhold C,Mufazalov IA,Ben-Nun A,Kuhlmann T,Wettschureck

    更新日期:2017-01-31 00:00:00

  • The Long Non-coding RNA lnc-31 Interacts with Rock1 mRNA and Mediates Its YB-1-Dependent Translation.

    abstract::Cytoplasmic long non-coding RNAs have been shown to act at many different levels to control post-transcriptional gene expression, although their role in translational control is poorly understood. Here, we show that lnc-31, a non-coding RNA required for myoblast proliferation, promotes ROCK1 protein synthesis by stabi...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Dimartino D,Colantoni A,Ballarino M,Martone J,Mariani D,Danner J,Bruckmann A,Meister G,Morlando M,Bozzoni I

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  • Nutrient-Dependent Trade-Offs between Ribosomes and Division Protein Synthesis Control Bacterial Cell Size and Growth.

    abstract::Cell size control emerges from a regulated balance between the rates of cell growth and division. In bacteria, simple quantitative laws connect cellular growth rate to ribosome abundance. However, it remains poorly understood how translation regulates bacterial cell size and shape under growth perturbations. Here, we ...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Serbanescu D,Ojkic N,Banerjee S

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  • FMRP-PKA Activity Negative Feedback Regulates RNA Binding-Dependent Fibrillation in Brain Learning and Memory Circuitry.

    abstract::Fragile X mental retardation protein (FMRP) promotes cyclic AMP (cAMP) signaling. Using an in vivo protein kinase A activity sensor (PKA-SPARK), we find that Drosophila FMRP (dFMRP) and human FMRP (hFMRP) enhance PKA activity in a central brain learning and memory center. Increasing neuronal PKA activity suppresses FM...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Sears JC,Broadie K

    更新日期:2020-10-13 00:00:00

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    abstract::The neural network of the temporal lobe is thought to provide a cognitive map of our surroundings. Functional analysis of this network has been hampered by coarse tools that often result in collateral damage to other circuits. We developed a chemogenetic system to temporally control electrical input into the hippocamp...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Zhao R,Grunke SD,Keralapurath MM,Yetman MJ,Lam A,Lee TC,Sousounis K,Jiang Y,Swing DA,Tessarollo L,Ji D,Jankowsky JL

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  • A De Novo Mutation in the Sodium-Activated Potassium Channel KCNT2 Alters Ion Selectivity and Causes Epileptic Encephalopathy.

    abstract::Early infantile epileptic encephalopathies (EOEE) are a debilitating spectrum of disorders associated with cognitive impairments. We present a clinical report of a KCNT2 mutation in an EOEE patient. The de novo heterozygous variant Phe240Leu SLICK was identified by exome sequencing and confirmed by Sanger sequencing. ...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Gururaj S,Palmer EE,Sheehan GD,Kandula T,Macintosh R,Ying K,Morris P,Tao J,Dias KR,Zhu Y,Dinger ME,Cowley MJ,Kirk EP,Roscioli T,Sachdev R,Duffey ME,Bye A,Bhattacharjee A

    更新日期:2017-10-24 00:00:00

  • Sex Differences in the Blood Transcriptome Identify Robust Changes in Immune Cell Proportions with Aging and Influenza Infection.

    abstract::Sex differences in autoimmunity and infection suggest that a better understanding of molecular sex differences will improve the diagnosis and treatment of immune-related disease. We identified 144 differentially expressed genes, referred to as immune sex expression signature (iSEXS), between human males and females us...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Bongen E,Lucian H,Khatri A,Fragiadakis GK,Bjornson ZB,Nolan GP,Utz PJ,Khatri P

    更新日期:2019-11-12 00:00:00

  • BLM Potentiates c-Jun Degradation and Alters Its Function as an Oncogenic Transcription Factor.

    abstract::Mutations in BLM helicase predispose Bloom syndrome (BS) patients to a wide spectrum of cancers. We demonstrate that MIB1-ubiquitylated BLM in G1 phase functions as an adaptor protein by enhancing the binding of transcription factor c-Jun and its E3 ligase, Fbw7α. BLM enhances the K48/K63-linked ubiquitylation on c-Ju...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Priyadarshini R,Hussain M,Attri P,Kaur E,Tripathi V,Priya S,Dhapola P,Saha D,Madhavan V,Chowdhury S,Sengupta S

    更新日期:2018-07-24 00:00:00

  • SIRT7 represses Myc activity to suppress ER stress and prevent fatty liver disease.

    abstract::Nonalcoholic fatty liver disease is the most common chronic liver disorder in developed countries. Its pathogenesis is poorly understood, and therapeutic options are limited. Here, we show that SIRT7, an NAD(+)-dependent H3K18Ac deacetylase, functions at chromatin to suppress ER stress and prevent the development of f...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Shin J,He M,Liu Y,Paredes S,Villanova L,Brown K,Qiu X,Nabavi N,Mohrin M,Wojnoonski K,Li P,Cheng HL,Murphy AJ,Valenzuela DM,Luo H,Kapahi P,Krauss R,Mostoslavsky R,Yancopoulos GD,Alt FW,Chua KF,Chen D

    更新日期:2013-11-14 00:00:00

  • Human C6orf211 encodes Armt1, a protein carboxyl methyltransferase that targets PCNA and is linked to the DNA damage response.

    abstract::Recent evidence supports the presence of an L-glutamyl methyltransferase(s) in eukaryotic cells, but this enzyme class has been defined only in certain prokaryotic species. Here, we characterize the human C6orf211 gene product as "acidic residue methyltransferase-1" (Armt1), an enzyme that specifically targets prolife...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Perry JJ,Ballard GD,Albert AE,Dobrolecki LE,Malkas LH,Hoelz DJ

    更新日期:2015-03-03 00:00:00

  • Manipulating Hippocampal Place Cell Activity by Single-Cell Stimulation in Freely Moving Mice.

    abstract::Learning critically depends on the ability to rapidly form and store non-overlapping representations of the external world. In line with their postulated role in episodic memory, hippocampal place cells can undergo a rapid reorganization of their firing fields upon contextual manipulations. To explore the mechanisms u...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Diamantaki M,Coletta S,Nasr K,Zeraati R,Laturnus S,Berens P,Preston-Ferrer P,Burgalossi A

    更新日期:2018-04-03 00:00:00

  • Identification and Structure of a Multidonor Class of Head-Directed Influenza-Neutralizing Antibodies Reveal the Mechanism for Its Recurrent Elicitation.

    abstract::Multidonor antibodies are of interest for vaccine design because they can in principle be elicited in the general population by a common set of immunogens. For influenza, multidonor antibodies have been observed against the hemagglutinin (HA) stem, but not the immunodominant HA head. Here, we identify and characterize...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Cheung CS,Fruehwirth A,Paparoditis PCG,Shen CH,Foglierini M,Joyce MG,Leung K,Piccoli L,Rawi R,Silacci-Fregni C,Tsybovsky Y,Verardi R,Wang L,Wang S,Yang ES,Zhang B,Zhang Y,Chuang GY,Corti D,Mascola JR,Shapiro L,K

    更新日期:2020-09-01 00:00:00

  • Microglial Displacement of GABAergic Synapses Is a Protective Event during Complex Febrile Seizures.

    abstract::Complex febrile seizures (FSs) lead to a high risk of intractable temporal lobe epilepsy during adulthood, yet the pathological process of complex FSs is largely unknown. Here, we demonstrate that activated microglia extensively associated with glutamatergic neuronal soma displace surrounding GABAergic presynapses in ...

    journal_title:Cell reports

    pub_type: 杂志文章


    authors: Wan Y,Feng B,You Y,Yu J,Xu C,Dai H,Trapp BD,Shi P,Chen Z,Hu W

    更新日期:2020-11-03 00:00:00