Abstract:
:Riboswitches are mRNA elements regulating gene expression in response to direct binding of a metabolite. While these RNAs are increasingly well understood with respect to interactions between receptor domains and their cognate effector molecules, little is known about the specific mechanistic relationship between metabolite binding and gene regulation by the downstream regulatory domain. Using a combination of cell-based, biochemical, and biophysical techniques, we reveal the specific RNA architectural features enabling a cobalamin-dependent hairpin loop docking interaction between receptor and regulatory domains. Furthermore, these data demonstrate that docking kinetics dictate a regulatory response involving the coupling of translation initiation to general mechanisms that control mRNA abundance. These results yield a comprehensive picture of how RNA structure in the riboswitch regulatory domain enables kinetically constrained ligand-dependent regulation of gene expression.
journal_name
Cell Repjournal_title
Cell reportsauthors
Polaski JT,Holmstrom ED,Nesbitt DJ,Batey RTdoi
10.1016/j.celrep.2016.03.087subject
Has Abstractpub_date
2016-05-03 00:00:00pages
1100-1110issue
5issn
2211-1247pii
S2211-1247(16)30393-Xjournal_volume
15pub_type
杂志文章相关文献
Cell Reports文献大全abstract::Large cohorts of human induced pluripotent stem cells (iPSCs) from healthy donors are a potentially powerful tool for investigating the relationship between genetic variants and cellular behavior. Here, we integrate high content imaging of cell shape, proliferation, and other phenotypes with gene expression and DNA se...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.01.094
更新日期:2019-02-19 00:00:00
abstract::Chromothripsis represents a novel phenomenon in the structural variation landscape of cancer genomes. Here, we analyze the genomes of ten patients with congenital disease who were preselected to carry complex chromosomal rearrangements with more than two breakpoints. The rearrangements displayed unanticipated complexi...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2012.05.009
更新日期:2012-06-28 00:00:00
abstract::Transcription factors often activate and repress different target genes in the same cell. How activation and repression are encoded by different arrangements of transcription factor binding sites in cis-regulatory elements is poorly understood. We investigated how sites for the transcription factor CRX encode both act...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.09.066
更新日期:2016-10-25 00:00:00
abstract::Molecular and behavioral responses to opioids are thought to be primarily mediated by neurons, although there is accumulating evidence that other cell types play a prominent role in drug addiction. To investigate cell-type-specific opioid responses, we performed single-cell RNA sequencing (scRNA-seq) of the nucleus ac...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.08.080
更新日期:2018-09-25 00:00:00
abstract::The diversity and complexity of the cancer transcriptome may contain transcripts unique to the tumor environment. Here, we report a LIN28B variant, LIN28B-TST, which is specifically expressed in hepatocellular carcinoma (HCC) and many other cancer types. Expression of LIN28B-TST is associated with significantly poor p...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.02.002
更新日期:2018-02-20 00:00:00
abstract::Many animals fight for dominance between conspecifics. Because winners could obtain more resources than losers, fighting outcomes are important for the animal's survival, especially in a situation with insufficient resources, such as hunger. However, it remains unclear whether and how hunger affects fighting outcomes....
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.107790
更新日期:2020-06-23 00:00:00
abstract::Complex interactions between immune cells are an important component in the induction of obesity. Here, we show that Il2rg-/-Rag2-/- mice lacking all lymphocytes are resistant to diet-induced obesity. Transplantation of bone marrow cells from Rag2-/- mice, which lack only acquired immune cells, into Il2rg-/-Rag2-/- mi...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.06.016
更新日期:2019-07-02 00:00:00
abstract::Sex differences in autoimmunity and infection suggest that a better understanding of molecular sex differences will improve the diagnosis and treatment of immune-related disease. We identified 144 differentially expressed genes, referred to as immune sex expression signature (iSEXS), between human males and females us...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.10.019
更新日期:2019-11-12 00:00:00
abstract::The role of neurotrophic factors as endogenous survival proteins for brain neurons remains contentious. In the cerebellum, the signals controlling survival of molecular layer interneurons (MLIs) are unknown, and direct evidence for the requirement of a full complement of MLIs for normal cerebellar function and motor l...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.05.030
更新日期:2017-06-06 00:00:00
abstract::The transmission of malaria parasites to mosquitoes relies on the rapid induction of sexual reproduction upon their ingestion into a blood meal. Haploid female and male gametocytes become activated and emerge from their host cells, and the males enter the cell cycle to produce eight microgametes. The synchronized natu...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.10.071
更新日期:2017-11-14 00:00:00
abstract::The hormone αKlotho regulates lifespan in mice, as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast, the overexpression of αKlotho increases lifespan. Given the severe mouse phenotype, we generated zebrafish mutants for αklotho as well as i...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.08.013
更新日期:2019-09-10 00:00:00
abstract::Maintaining organelle function in the face of stress is known to involve organelle-specific retrograde signaling. Using Caenorhabditis elegans, we present evidence of the existence of such retrograde signaling for peroxisomes, which we define as the peroxisomal retrograde signaling (PRS). Specifically, we show that pe...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108653
更新日期:2021-01-19 00:00:00
abstract::Diverse cell types can be reprogrammed into pluripotent stem cells by ectopic expression of Oct4 (Pou5f1), Klf4, Sox3, and Myc. Many of these induced pluripotent stem cells (iPSCs) retain memory, in terms of DNA methylation and histone modifications (epigenetic memory), of their cellular origins, and this may bias sub...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.01.075
更新日期:2018-03-06 00:00:00
abstract::Oligodendrogenesis occurs during early postnatal development, coincident with neurogenesis and synaptogenesis, raising the possibility that microglia-dependent pruning mechanisms that modulate neurons regulate myelin sheath formation. Here we show a population of ameboid microglia migrating from the ventricular zone i...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108047
更新日期:2020-08-18 00:00:00
abstract::Oxytocin and its receptor (Oxtr) play a crucial role in the postnatal transition of neuronal GABA neurotransmission from excitatory to inhibitory, a developmental process known as the GABA switch. Using hippocampal neurons from Oxtr-null mice, we show that (1) Oxtr is necessary for the correct timing of the GABA switc...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.03.013
更新日期:2016-04-05 00:00:00
abstract::Bin-Amphiphysin-Rvs (BAR) domain proteins are central regulators of many cellular processes involving membrane dynamics. BAR domains sculpt phosphoinositide-rich membranes to generate membrane protrusions or invaginations. Here, we report that, in addition to regulating membrane geometry, BAR domains can generate extr...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.08.024
更新日期:2013-09-26 00:00:00
abstract::The kidney has a tremendous capacity to regenerate following injury, but factors that govern this response are still largely unknown. We isolated cells from mouse kidneys with high proliferative and multi-lineage differentiation capacity. These cells expressed a high level of Sox9. In regenerating kidneys, Sox9 expres...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.12.071
更新日期:2016-02-02 00:00:00
abstract::A mature olfactory sensory neuron (OSN) of the main olfactory epithelium (MOE) typically expresses one allele of one odorant receptor (OR) gene. It is widely thought that the great majority of the 1,141 intact mouse OR genes are expressed in one of four MOE zones (or bands or stripes), which are largely non-overlappin...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.02.110
更新日期:2020-03-24 00:00:00
abstract::Oxytocin is a hormone with various actions. Oxytocin-containing parvocellular neurons project to the brainstem and spinal cord. Oxytocin release from these neurons suppresses nociception of inflammatory pain, the molecular mechanism of which remains unclear. Here, we report that the noxious stimulus receptor TRPV1 is ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.10.063
更新日期:2017-11-07 00:00:00
abstract::Nephrons, the functional units of the kidney, develop from progenitor cells (cap mesenchyme [CM]) surrounding the epithelial ureteric bud (UB) tips. Reciprocal signaling between UB and CM induces nephrogenesis and UB branching. Although low nephron number is implicated in hypertension and renal disease, the mechanisms...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.02.033
更新日期:2014-04-10 00:00:00
abstract::Inhibition of the ATPase cycle of the HSP90 chaperone promotes ubiquitylation and proteasomal degradation of its client proteins, which include many oncogenic protein kinases. This provides the rationale for HSP90 inhibitors as cancer therapeutics. However, the mechanism by which HSP90 ATPase inhibition triggers ubiqu...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.05.078
更新日期:2017-06-20 00:00:00
abstract::The flat bones of the skull are densely innervated during development, but little is known regarding their role during repair. We describe a neurotrophic mechanism that directs sensory nerve transit in the mouse calvaria. Patent cranial suture mesenchyme represents an NGF (nerve growth factor)-rich domain, in which se...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.107696
更新日期:2020-05-26 00:00:00
abstract::The circadian clock generates biological rhythms of metabolic and physiological processes, including the sleep-wake cycle. We previously identified a missense mutation in the flavin adenine dinucleotide (FAD) binding pocket of CRYPTOCHROME2 (CRY2), a clock protein that causes human advanced sleep phase. This prompted ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.03.041
更新日期:2017-04-11 00:00:00
abstract::Development and function of pancreatic β cells involve the regulated activity of specific transcription factors. RFX6 is a transcription factor essential for mouse β cell differentiation that is mutated in monogenic forms of neonatal diabetes. However, the expression and functional roles of RFX6 in human β cells, espe...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.11.010
更新日期:2014-12-24 00:00:00
abstract::Brain networks contain a large diversity of functionally distinct neuronal elements, each with unique properties, enabling computational capacities and supporting brain functions. Understanding their functional implications for behavior requires the precise identification of the cell types of a network and in vivo mon...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.11.042
更新日期:2014-12-24 00:00:00
abstract::Simultaneous expression of Oct4, Klf4, Sox2, and cMyc induces pluripotency in somatic cells (iPSCs). Replacing Oct4 with the neuro-specific factor Brn4 leads to transdifferentiation of fibroblasts into induced neural stem cells (iNSCs). However, Brn4 was recently found to induce transient acquisition of pluripotency b...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.03.023
更新日期:2019-04-02 00:00:00
abstract::Folates are cofactors for biosynthetic enzymes in all eukaryotic and prokaryotic cells. Animals cannot synthesize folate and must acquire it from their diet or microbiota. Previously, we showed that inhibiting E. coli folate synthesis increases C. elegans lifespan. Here, we show that restriction or supplementation of ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.01.051
更新日期:2016-02-23 00:00:00
abstract::In spinal muscular atrophy, a neurodegenerative disease caused by ubiquitous deficiency in the survival motor neuron (SMN) protein, sensory-motor synaptic dysfunction and increased excitability precede motor neuron (MN) loss. Whether central synaptic dysfunction and MN hyperexcitability are cell-autonomous events or t...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.06.087
更新日期:2016-08-02 00:00:00
abstract::In glioblastoma, invasion and proliferation are presumed to be mutually exclusive events; however, the molecular mechanisms that mediate this switch at the cellular level remain elusive. Previously, we have shown that phospho-OLIG2, a central-nervous-system-specific transcription factor, is essential for tumor growth ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.06.045
更新日期:2016-07-26 00:00:00
abstract::Physicians have observed that surgical wounds in the elderly heal with thinner scars than wounds in young patients. Understanding this phenomenon may reveal strategies for promoting scarless wound repair. We show that full-thickness skin wounds in aged but not young mice fully regenerate. Exposure of aged animals to b...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.08.054
更新日期:2018-09-25 00:00:00