Abstract：
:The basement membrane (BM), a sheet of extracellular matrix lining the basal side of epithelia, is essential for epithelial cell function and integrity, yet the mechanisms that control the basal restriction of BM proteins are poorly understood. In epithelial cells, a specialized pathway is dedicated to restrict the deposition of BM proteins basally. Here, we report the identification of a factor in this pathway, a homolog of the mammalian guanine nucleotide exchange factor (GEF) Mss4, which we have named Stratum. The loss of Stratum leads to the missecretion of BM proteins at the apical side of the cells, forming aberrant layers in close contact with the plasma membrane. We found that Rab8GTPase acts downstream of Stratum in this process. Altogether, our results uncover the importance of this GEF/Rab complex in specifically coordinating the basal restriction of BM proteins, a critical process for the establishment and maintenance of epithelial cell polarity.
journal_name
Cell Repjournal_title
Cell reportsauthors
Devergne O,Sun GH,Schüpbach Tdoi
10.1016/j.celrep.2017.02.002subject
Has Abstractpub_date
2017-02-21 00:00:00pages
1831-1839issue
8issn
2211-1247pii
S2211-1247(17)30167-5journal_volume
18pub_type
杂志文章相关文献
Cell Reports文献大全abstract：:Chlamydia trachomatis is a human pathogen associated with significant morbidity worldwide. As obligate intracellular parasites, chlamydiae must survive within eukaryotic cells for sufficient time to complete their developmental cycle. To promote host cell survival, chlamydiae express poorly understood anti-apoptotic f...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.04.058
更新日期：2017-05-16 00:00:00
abstract：:As three-dimensional cell culture formats gain in popularity, there emerges a need for tools that produce vast amounts of data on individual cells within the spheroids or organoids. Here, we present a microfluidic platform that provides access to such data by parallelizing the manipulation of individual spheroids with...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107670
更新日期：2020-05-26 00:00:00
abstract：:Apolipoprotein M (apoM) is the carrier of sphingosine-1-phosphate (S1P) in plasma high-density lipoproteins. S1P is a bioactive lipid interacting with five receptors (S1P1-5). We show that lack of apoM in mice increases the amount of brown adipose tissue (BAT), accelerates the clearance of postprandial triglycerides, ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.12.029
更新日期：2018-01-02 00:00:00
abstract：:The resolution phase of acute inflammation is essential for tissue homeostasis, yet the underlying mechanisms remain unclear. We demonstrate that resolution of inflammation involves interactions between CD38 and tristetraprolin (TTP). During the onset of acute inflammation, CD38 levels are increased, leading to the pr...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.12.090
更新日期：2020-01-28 00:00:00
abstract：:Neutrophils are short-lived blood cells that play a critical role in host defense against infections. To better comprehend neutrophil functions and their regulation, we provide a complete epigenetic overview, assessing important functional features of their differentiation stages from bone marrow-residing progenitors ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.08.018
更新日期：2018-09-04 00:00:00
abstract：:ADP-ribosylation (ADPr) is a reversible posttranslational modification involved in a range of cellular processes. Here, we report system-wide identification of serine ADPr in human cells upon oxidative stress. High-resolution mass spectrometry and unrestricted data processing confirm that serine residues are the major...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.07.083
更新日期：2018-08-28 00:00:00
abstract：:In Escherichia coli, bidirectional chromosomal replication is accompanied by the colocalization of sister replication forks. However, the biological significance of this mechanism and the key factors involved are still largely unknown. In this study, we found that a protein, termed CrfC, helps sustain the colocalizati...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.07.040
更新日期：2013-09-12 00:00:00
abstract：:The newest classes of anti-diabetic agents include sodium-glucose cotransporter 2 (SGLT2) inhibitors and glucagon-like peptide 1 receptor (GLP1R) agonists. The SGLT2 inhibitor dapagliflozin reduces glucotoxicity by glycosuria but elevates glucagon secretion. The GLP1R agonist liraglutide inhibits glucagon; therefore, ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.07.009
更新日期：2019-08-06 00:00:00
abstract：:Naturally acquired immunity to malaria develops only after years of repeated exposure to Plasmodium parasites. Despite the key role antibodies play in protection, the cellular processes underlying the slow acquisition of immunity remain unknown. Using mouse models, we show that severe malaria infection inhibits the es...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.12.006
更新日期：2016-01-05 00:00:00
abstract：:Oxytocin is a hormone with various actions. Oxytocin-containing parvocellular neurons project to the brainstem and spinal cord. Oxytocin release from these neurons suppresses nociception of inflammatory pain, the molecular mechanism of which remains unclear. Here, we report that the noxious stimulus receptor TRPV1 is ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.10.063
更新日期：2017-11-07 00:00:00
abstract：:Transport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis of specific proteins at active synapses on demand, thereby contributing to learning and memory. To gain insight into the machinery controlling dendritic mRNA localization and translation, we established a stringent protocol to...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.11.023
更新日期：2013-12-26 00:00:00
abstract：:Cyclin-dependent kinases (CDKs) contribute to vital cellular processes including cell cycle regulation. Loss of CDKs is associated with impaired insulin secretion and β cell survival; however, the function of CDK8 in β cells remains elusive. Here, we report that genetic ablation of Cdk8 improves glucose tolerance by i...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.08.025
更新日期：2019-09-10 00:00:00
abstract：:CRISPR-Cas13a enzymes are RNA-guided, RNA-activated RNases. Their properties have been exploited as powerful tools for RNA detection, RNA imaging, and RNA regulation. However, the relationship between target RNA binding and HEPN (higher eukaryotes and prokaryotes nucleotide binding) domain nuclease activation is poorl...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.06.105
更新日期：2018-07-24 00:00:00
abstract：:X-linked inhibitor of apoptosis protein (XIAP) has been identified as a potent regulator of innate immune responses, and loss-of-function mutations in XIAP cause the development of the X-linked lymphoproliferative syndrome type 2 (XLP-2) in humans. Using gene-targeted mice, we show that loss of XIAP or deletion of its...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.05.008
更新日期：2014-06-26 00:00:00
abstract：:Aging strongly influences human morbidity and mortality. Thus, aging-preventive compounds could greatly improve our health and lifespan. Here we screened for such compounds, known as geroprotectors, employing the power of transcriptomics to predict biological age. Using age-stratified human tissue transcriptomes and m...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.03.044
更新日期：2019-04-09 00:00:00
abstract：:Oct4 is an essential regulator of pluripotency in vivo and in vitro in embryonic stem cells, as well as a key mediator of the reprogramming of somatic cells into induced pluripotent stem cells. It is not known whether activation and/or repression of specific genes by Oct4 is relevant to these functions. Here, we show ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2011.12.002
更新日期：2012-02-23 00:00:00
abstract：:Brain function is compromised in myotonic dystrophy type 1 (DM1), but the underlying mechanisms are not fully understood. To gain insight into the cellular and molecular pathways primarily affected, we studied a mouse model of DM1 and brains of adult patients. We found pronounced RNA toxicity in the Bergmann glia of t...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.06.006
更新日期：2017-06-27 00:00:00
abstract：:MicroRNA (miRNA) transfer via exosomes may mediate cell-to-cell communication. Interestingly, specific miRNAs are enriched in exosomes in a cell-type-dependent fashion. However, the mechanisms whereby miRNAs are sorted to exosomes and the significance of miRNA transfer to acceptor cells are unclear. We used macrophage...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.07.035
更新日期：2014-09-11 00:00:00
abstract：:Glycophosphatidylinositol-anchored proteins (GPI-APs) play essential roles in physiology, but their biogenesis and trafficking have not been systematically characterized. Here, we took advantage of the recently available haploid genetics approach to dissect GPI-AP pathways in human cells using prion protein (PrP) and ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.05.026
更新日期：2015-06-23 00:00:00
abstract：:Cytotoxic CD8(+) T lymphocytes play a critical role in the host response to infection by viruses. The ability to secrete cytotoxic chemicals and cytokines is considered pivotal for eliminating virus. Of equal importance is how effector CD8(+) T cells home to virus-infected tissues. L-selectin has not been considered i...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.12.090
更新日期：2016-02-02 00:00:00
abstract：:Mural cells (pericytes and vascular smooth muscle cells) are essential for the regulation of vascular networks and maintenance of vascular integrity, but their origins are diverse in different tissues and not known in the organs that arise from the ectoderm, such as skin. Here, we show that tissue-localized myeloid pr...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.02.069
更新日期：2017-03-21 00:00:00
abstract：:One of the most notable characteristics of synaptic transmission is the wide variation in synaptic strength in response to identical stimulation. In hippocampal neurons, approximately one-third of axonal mitochondria are highly motile, and some dynamically pass through presynaptic boutons. This raises a fundamental qu...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.06.040
更新日期：2013-08-15 00:00:00
abstract：:The vitamin A metabolite retinoic acid (RA) provides patterning information during vertebrate embryogenesis, but the mechanism through which RA influences limb development is unclear. During patterning of the limb proximodistal axis (upper limb to digits), avian studies suggest that a proximal RA signal generated in t...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.03.036
更新日期：2013-05-30 00:00:00
abstract：:Synapses are fundamental to the normal function of the nervous system. Glia play a pivotal role in regulating synaptic formation. However, how presynaptic neurons assemble synaptic structure in response to the glial signals remains largely unexplored. To address this question, we use cima-1 mutant C. elegans as an in ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.01.102
更新日期：2020-02-25 00:00:00
abstract：:Tripartite motif-containing protein 5α (TRIM5α) is a cellular antiviral restriction factor that prevents early events in retrovirus replication. The activity of TRIM5α is thought to be limited to retroviruses as a result of highly specific interactions with capsid lattices. In contrast to this current understanding, w...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.05.040
更新日期：2019-06-11 00:00:00
abstract：:During development, neurons are constantly refining their connections in response to changes in activity. Experience-dependent plasticity is a key form of synaptic plasticity, involving changes in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) accumulation at synapses. Here, we report a critical...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.04.054
更新日期：2014-06-12 00:00:00
abstract：:The endothelial monolayer forms a barrier between the lumen of blood vessels and the underlying tissues. Stable VE-cadherin-based adherens junctions are essential for maintaining this barrier, whereas their remodeling is required for angiogenesis in health and disease. Here, we position the ERAD-associated ubiquitin l...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107944
更新日期：2020-08-04 00:00:00
abstract：:The timing of food intake and nutrient utilization is critical to health and regulated partly by the circadian clock. Increased amplitude of circadian oscillations and metabolic output has been found to improve health in diabetic and obesity mouse models. Here, we report a function for the circadian deadenylase Noctur...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.01.011
更新日期：2018-01-30 00:00:00
abstract：:Over 80% of pancreatic ductal adenocarcinoma (PDA) patients are diagnosed with non-resectable late-stage disease that lacks effective neoadjuvant therapies. Stereotactic body radiation therapy (SBRT) has shown promise as an emerging neoadjuvant approach for treating PDA, and here, we report that its combination with l...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.08.095
更新日期：2019-10-08 00:00:00
abstract：:Molecular and behavioral responses to opioids are thought to be primarily mediated by neurons, although there is accumulating evidence that other cell types play a prominent role in drug addiction. To investigate cell-type-specific opioid responses, we performed single-cell RNA sequencing (scRNA-seq) of the nucleus ac...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.08.080
更新日期：2018-09-25 00:00:00
