Abstract：
:In neurons, transmembrane proteins are targeted to dendrites in vesicles that traffic solely within the somatodendritic compartment. How these vesicles are retained within the somatodendritic domain is unknown. Here, we use a novel pulse-chase system, which allows synchronous release of exogenous transmembrane proteins from the endoplasmic reticulum to follow movements of post-Golgi transport vesicles. Surprisingly, we found that post-Golgi vesicles carrying dendritic proteins were equally likely to enter axons and dendrites. However, once such vesicles entered the axon, they very rarely moved beyond the axon initial segment but instead either halted or reversed direction in an actin and Myosin Va-dependent manner. In contrast, vesicles carrying either an axonal or a nonspecifically localized protein only rarely halted or reversed and instead generally proceeded to the distal axon. Thus, our results are consistent with the axon initial segment behaving as a vesicle filter that mediates the differential trafficking of transport vesicles.
journal_name
Cell Repjournal_title
Cell reportsauthors
Al-Bassam S,Xu M,Wandless TJ,Arnold DBdoi
10.1016/j.celrep.2012.05.018subject
Has Abstractpub_date
2012-07-26 00:00:00pages
89-100issue
1issn
2211-1247pii
S2211-1247(12)00157-Xjournal_volume
2pub_type
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