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FACS purification and transcriptome analysis of drosophila neural stem cells reveals a role for Klumpfuss in self-renewal.

Abstract：

:Drosophila neuroblasts (NBs) have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type-specific gene expression data. Here, we describe a method for isolating large numbers of pure NBs and differentiating neurons that retain both cell-cycle and lineage characteristics. We determine transcriptional profiles by mRNA sequencing and identify 28 predicted NB-specific transcription factors that can be arranged in a network containing hubs for Notch signaling, growth control, and chromatin regulation. Overexpression and RNA interference for these factors identify Klumpfuss as a regulator of self-renewal. We show that loss of Klumpfuss function causes premature differentiation and that overexpression results in the formation of transplantable brain tumors. Our data represent a valuable resource for investigating Drosophila developmental neurobiology, and the described method can be applied to other invertebrate stem cell lineages as well.

journal_name

Cell Rep

journal_title

Cell reports

authors

Berger C,Harzer H,Burkard TR,Steinmann J,van der Horst S,Laurenson AS,Novatchkova M,Reichert H,Knoblich JA

doi

10.1016/j.celrep.2012.07.008

subject

Has Abstract

pub_date

2012-08-30 00:00:00

pages

407-18

issue

2

issn

2211-1247

pii

S2211-1247(12)00218-5

journal_volume

2

pub_type

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