Abstract:
:RIP1 is a central mediator of cell death in response to cell stress but can also mediate cell survival by activating NF-κB. Here, we show that RIP1 acts as a switch in EGFR signaling. EGFRvIII is an oncogenic mutant that does not bind ligand and is coexpressed with EGFRWT in glioblastoma multiforme (GBM). EGFRvIII recruits ubiquitin ligases to RIP1, resulting in K63-linked ubiquitination of RIP1. RIP1 binds to TAK1 and NEMO, forming an EGFRvIII-RIP1 signalosome that activates NF-κB. RIP1 is essential for EGFRvIII-mediated oncogenicity and correlates with NF-κB activation in GBM. Surprisingly, activation of EGFRWT with EGF results in a negative regulation of EGFRvIII, with dissociation of the EGFRvIII-RIP1 signalosome, loss of RIP1 ubiquitination and NF-κB activation, and association of RIP1 with FADD and caspase-8. If EGFRWT is overexpressed with EGFRvIII, the addition of EGF leads to a RIP1 kinase-dependent cell death. The EGFRWT-EGFRvIII-RIP1 interplay may regulate oncogenicity and vulnerability to targeted treatment in GBM.
journal_name
Cell Repjournal_title
Cell reportsauthors
Puliyappadamba VT,Chakraborty S,Chauncey SS,Li L,Hatanpaa KJ,Mickey B,Noorani S,Shu HK,Burma S,Boothman DA,Habib AAdoi
10.1016/j.celrep.2013.07.025subject
Has Abstractpub_date
2013-08-29 00:00:00pages
764-75issue
4issn
2211-1247pii
S2211-1247(13)00387-2journal_volume
4pub_type
杂志文章相关文献
Cell Reports文献大全abstract::Synapsin III (SynIII) is a phosphoprotein that is highly expressed at early stages of neuronal development. Whereas in vitro evidence suggests a role for SynIII in neuronal differentiation, in vivo evidence is lacking. Here, we demonstrate that in vivo downregulation of SynIII expression affects neuronal migration and...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.03.022
更新日期:2015-04-14 00:00:00
abstract::Preventing transcription-replication head-on conflict (HO-TRC)-triggered R-loop formation is essential for maintaining genome integrity in bacteria, plants, and mammals. The R-loop eraser RNase H can efficiently relax HO-TRCs. However, it is not clear how organisms resist HO-TRC-triggered R-loops when RNase H proteins...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.12.007
更新日期:2020-01-07 00:00:00
abstract::The newest classes of anti-diabetic agents include sodium-glucose cotransporter 2 (SGLT2) inhibitors and glucagon-like peptide 1 receptor (GLP1R) agonists. The SGLT2 inhibitor dapagliflozin reduces glucotoxicity by glycosuria but elevates glucagon secretion. The GLP1R agonist liraglutide inhibits glucagon; therefore, ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.07.009
更新日期:2019-08-06 00:00:00
abstract::In mammalian skin, Merkel cells are mechanoreceptor cells that are required for the perception of gentle touch. Recent evidence indicates that mature Merkel cells descend from the proliferative layer of skin epidermis; however, the stem cell niche for Merkel cell homeostasis has not been reported. Here, we provide gen...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.04.026
更新日期:2013-06-27 00:00:00
abstract::Most current tumor immunotherapy strategies leverage cytotoxic CD8+ T cells. Despite evidence for clinical potential of CD4+ tumor-infiltrating lymphocytes (TILs), their functional diversity limits our ability to harness their activity. Here, we use single-cell mRNA sequencing to analyze the response of tumor-specific...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.10.131
更新日期:2019-12-03 00:00:00
abstract::Parkinson's disease (PD) is a common neurodegenerative disorder that exhibits motor and non-motor symptoms, as well as pathological hallmarks, including dopaminergic (DA) neuron death and formation of α-synuclein (α-Syn) Lewy bodies. Cyclin-G-associated kinase (GAK), a PD susceptibility gene identified through genome-...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.01.005
更新日期:2017-01-31 00:00:00
abstract::In higher vertebrates, cephalic neural crest cells (NCCs) form craniofacial skeleton by differentiating into chondrocytes and osteoblasts. A subpopulation of cephalic NCCs, cardiac NCCs (CNCCs), migrates to the heart. However, CNCCs mostly do not yield skeletogenic derivatives, and the molecular mechanisms of this fat...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.09.019
更新日期:2019-10-15 00:00:00
abstract::The innate immune system senses cytosolic dsDNA and bacterial cyclic dinucleotides and initiates signaling via the adaptor STING to induce type 1 interferon (IFN) response. We demonstrate here that BTK-deficient cells have impaired IFN-β production and TBK1/IRF3 activation when stimulated with agonists or infected wit...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.01.039
更新日期:2015-02-24 00:00:00
abstract::A role for cancer cell epithelial-to-mesenchymal transition (EMT) in cancer is well established. Here, we show that, in addition to cancer cell EMT, ovarian cancer cell metastasis relies on an epigenomic mesenchymal-to-epithelial transition (MET) in host mesenchymal stem cells (MSCs). These reprogrammed MSCs, termed c...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108473
更新日期:2020-12-08 00:00:00
abstract::Glucagon, the principal hyperglycemic hormone, is secreted from pancreatic islet α cells as part of the counter-regulatory response to hypoglycemia. Hence, secretory output from α cells is under high demand in conditions of low glucose supply. Many tissues oxidize fat as an alternate energy substrate. Here, we show th...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.05.035
更新日期:2018-06-12 00:00:00
abstract::Polyunsaturated fatty acids (PUFAs) confer health benefits by preventing inflammation and obesity and by increasing thermogenesis in brown and beige adipocytes. As well as being supplied exogenously as nutrients, PUFAs are largely stored in membrane glycerophospholipids and released by phospholipase A2s (PLA2s). Howev...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.107579
更新日期:2020-05-05 00:00:00
abstract::Microglia have important remodeling functions in neurodevelopment, aging, and disease, with evidence for molecular diversity. However, the signaling pathways and environmental cues that drive diverse states of microglia are incompletely understood. We profiled microglia of a discrete developing CNS region, the murine ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.04.062
更新日期:2019-05-14 00:00:00
abstract::Inactivation of the Pten tumor suppressor negatively regulates the PI3K-mTOR pathway. In a model of cutaneous squamous cell carcinoma (SCC), we demonstrate that deletion of Pten strongly elevates Fgf10 protein levels without increasing Fgf10 transcription in vitro and in vivo. The translational activation of Fgf10 by ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.01.045
更新日期:2014-03-13 00:00:00
abstract::Simultaneous expression of Oct4, Klf4, Sox2, and cMyc induces pluripotency in somatic cells (iPSCs). Replacing Oct4 with the neuro-specific factor Brn4 leads to transdifferentiation of fibroblasts into induced neural stem cells (iNSCs). However, Brn4 was recently found to induce transient acquisition of pluripotency b...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.03.023
更新日期:2019-04-02 00:00:00
abstract::Generation of insulin-secreting β cells in vitro is a promising approach for diabetes cell therapy. Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are differentiated to β cells (SC-β cells) and mature to undergo glucose-stimulated insulin secretion, but molecular regulation of thi...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.107687
更新日期:2020-05-26 00:00:00
abstract::Histone H3 lysine 79 (H3K79) methylation is enriched on actively transcribed genes, and its misregulation is a hallmark of leukemia. Methylation of H3K79, which resides on the structured disk face of the nucleosome, is mediated by the Dot1L methyltransferase. Dot1L activity is part of a trans-histone crosstalk pathway...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.01.058
更新日期:2019-02-12 00:00:00
abstract::The neural network of the temporal lobe is thought to provide a cognitive map of our surroundings. Functional analysis of this network has been hampered by coarse tools that often result in collateral damage to other circuits. We developed a chemogenetic system to temporally control electrical input into the hippocamp...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.06.032
更新日期:2016-07-19 00:00:00
abstract::RAS family GTPases contribute directly to the regulation of type I phosphoinositide 3-kinases (PI3Ks) via RAS-binding domains in the PI3K catalytic p110 subunits. Disruption of this domain of p110α impairs RAS-mutant-oncogene-driven tumor formation and maintenance. Here, we test the effect of blocking the interaction ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.12.003
更新日期:2018-12-26 00:00:00
abstract::The mammalian heart is incapable of regenerating a sufficient number of cardiomyocytes to ameliorate the loss of contractile muscle after acute myocardial injury. Several reports have demonstrated that mononucleated cardiomyocytes are more responsive than are binucleated cardiomyocytes to pro-proliferative stimuli. We...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.02.034
更新日期:2020-03-03 00:00:00
abstract::A central paradigm in the field of lymphocyte biology asserts that replicatively senescent memory T cells express the carbohydrate epitope CD57. These cells nonetheless accumulate with age and expand numerically in response to persistent antigenic stimulation. Here, we use in vivo deuterium labeling and ex vivo analys...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108501
更新日期:2020-12-15 00:00:00
abstract::Transport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis of specific proteins at active synapses on demand, thereby contributing to learning and memory. To gain insight into the machinery controlling dendritic mRNA localization and translation, we established a stringent protocol to...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.11.023
更新日期:2013-12-26 00:00:00
abstract::CISD2 is located within the chromosome 4q region frequently deleted in hepatocellular carcinoma (HCC). Mice with Cisd2 heterozygous deficiency develop a phenotype similar to the clinical manifestation of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Cisd2 haploinsufficiency causes a...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.10.099
更新日期:2017-11-21 00:00:00
abstract::Mutations in BLM helicase predispose Bloom syndrome (BS) patients to a wide spectrum of cancers. We demonstrate that MIB1-ubiquitylated BLM in G1 phase functions as an adaptor protein by enhancing the binding of transcription factor c-Jun and its E3 ligase, Fbw7α. BLM enhances the K48/K63-linked ubiquitylation on c-Ju...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.06.101
更新日期:2018-07-24 00:00:00
abstract::The fossil record is widely informative about evolution, but fossils are not systematically used to study the evolution of stem-cell-driven renewal. Here, we examined evolution of the continuous growth (hypselodonty) of rodent molar teeth, which is fuelled by the presence of dental stem cells. We studied occurrences o...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.03.064
更新日期:2015-05-05 00:00:00
abstract::It has been proposed that sleep's contribution to memory consolidation is to reactivate prior encoded information. To elucidate the neural mechanisms carrying reactivation-related mnemonic information, we investigated whether content-specific memory signatures associated with memory reactivation during wakefulness reo...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.09.037
更新日期:2018-10-09 00:00:00
abstract::Bat cells and tissue have elevated basal expression levels of antiviral genes commonly associated with interferon alpha (IFNα) signaling. Here, we show Interferon Regulatory Factor 1 (IRF1), 3, and 7 levels are elevated in most bat tissues and that, basally, IRFs contribute to the expression of type I IFN ligands and ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108345
更新日期:2020-11-03 00:00:00
abstract::Early infantile epileptic encephalopathies (EOEE) are a debilitating spectrum of disorders associated with cognitive impairments. We present a clinical report of a KCNT2 mutation in an EOEE patient. The de novo heterozygous variant Phe240Leu SLICK was identified by exome sequencing and confirmed by Sanger sequencing. ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.09.088
更新日期:2017-10-24 00:00:00
abstract::Recent evidence supports the presence of an L-glutamyl methyltransferase(s) in eukaryotic cells, but this enzyme class has been defined only in certain prokaryotic species. Here, we characterize the human C6orf211 gene product as "acidic residue methyltransferase-1" (Armt1), an enzyme that specifically targets prolife...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.01.054
更新日期:2015-03-03 00:00:00
abstract::APOBEC family cytidine deaminases have recently been implicated as powerful mutators of cancer genomes. How APOBECs, which are ssDNA-specific enzymes, gain access to chromosomal DNA is unclear. To ascertain the chromosomal ssDNA substrates of the APOBECs, we expressed APOBEC3A and APOBEC3B, the two most probable APOBE...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.01.021
更新日期:2016-02-16 00:00:00
abstract::The hormone αKlotho regulates lifespan in mice, as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast, the overexpression of αKlotho increases lifespan. Given the severe mouse phenotype, we generated zebrafish mutants for αklotho as well as i...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.08.013
更新日期:2019-09-10 00:00:00