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The architecture of yeast DNA polymerase ζ.

Abstract：

:DNA polymerase ζ (Polζ) is specialized for the extension step of translesion DNA synthesis (TLS). Despite its central role in maintaining genome integrity, little is known about its overall architecture. Initially identified as a heterodimer of the catalytic subunit Rev3 and the accessory subunit Rev7, yeast Polζ has recently been shown to form a stable four-subunit enzyme (Polζ-d) upon the incorporation of Pol31 and Pol32, the accessory subunits of yeast Polδ. To understand the 3D architecture and assembly of Polζ and Polζ-d, we employed electron microscopy. We show here how the catalytic and accessory subunits of Polζ and Polζ-d are organized relative to each other. In particular, we show that Polζ-d has a bilobal architecture resembling the replicative polymerases and that Pol32 lies in proximity to Rev7. Collectively, our study provides views of Polζ and Polζ-d and a structural framework for understanding their roles in DNA damage bypass.

journal_name

Cell Rep

journal_title

Cell reports

authors

Gómez-Llorente Y,Malik R,Jain R,Choudhury JR,Johnson RE,Prakash L,Prakash S,Ubarretxena-Belandia I,Aggarwal AK

doi

10.1016/j.celrep.2013.08.046

subject

Has Abstract

pub_date

2013-10-17 00:00:00

pages

79-86

issue

1

issn

2211-1247

pii

S2211-1247(13)00506-8

journal_volume

5

pub_type

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