Abstract：
:Synaptic efficacy plays crucial roles in neuronal circuit operation and synaptic plasticity. Presynaptic determinants of synaptic efficacy are neurotransmitter content in synaptic vesicles and the number of vesicles undergoing exocytosis at a time. Bursts of presynaptic firings depress synaptic efficacy, mainly due to depletion of releasable vesicles, whereas recovery from strong depression is initiated by endocytic vesicle retrieval followed by refilling of vesicles with neurotransmitter. We washed out presynaptic cytosolic GABA to induce a rundown of IPSCs at cerebellar inhibitory cell pairs in slices from rats and then allowed fast recovery by elevating GABA concentration using photo-uncaging. The time course of this recovery coincided with that of IPSCs from activity-dependent depression induced by a train of high-frequency stimulation. We conclude that vesicular GABA uptake can be a limiting step for the recovery of inhibitory neurotransmission from synaptic depression.
journal_name
Cell Repjournal_title
Cell reportsauthors
Yamashita M,Kawaguchi SY,Hori T,Takahashi Tdoi
10.1016/j.celrep.2018.02.080subject
Has Abstractpub_date
2018-03-20 00:00:00pages
3134-3141issue
12issn
2211-1247pii
S2211-1247(18)30290-0journal_volume
22pub_type
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