Abstract：
:At presynaptic terminals, neurotransmitters are released by synaptic vesicle exocytosis at the active zone. In order to maintain efficient neurotransmission and proper synaptic structure, sites of vesicle fusion must be cleared rapidly by endocytosis. Therefore, the coupling of exo- and endocytosis is crucial. Despite many years of research, the exact molecular and biophysical requirements for the coupling of exo- and endocytosis remain unclear. We investigate whether endocytosis can be triggered in a calcium-independent fashion by evoking calcium-independent exocytosis using a hypertonic sucrose solution. We demonstrate that endocytosis can be triggered, in the absence of calcium influx, in a clathrin-independent manner that relies on actin polymerization. Our findings point to a central role of membrane tension dependent on actin for efficient coupling of exo- and endocytosis.
journal_name
Cell Repjournal_title
Cell reportsauthors
Orlando M,Schmitz D,Rosenmund C,Herman MAdoi
10.1016/j.celrep.2019.11.060subject
Has Abstractpub_date
2019-12-17 00:00:00pages
3767-3774.e3issue
12issn
2211-1247pii
S2211-1247(19)31551-7journal_volume
29pub_type
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