Abstract:
:The RUNX genes encode transcription factors involved in development and human disease. RUNX1 and RUNX3 are frequently associated with leukemias, yet the basis for their involvement in leukemogenesis is not fully understood. Here, we show that Runx1;Runx3 double-knockout (DKO) mice exhibited lethal phenotypes due to bone marrow failure and myeloproliferative disorder. These contradictory clinical manifestations are reminiscent of human inherited bone marrow failure syndromes such as Fanconi anemia (FA), caused by defective DNA repair. Indeed, Runx1;Runx3 DKO cells showed mitomycin C hypersensitivity, due to impairment of monoubiquitinated-FANCD2 recruitment to DNA damage foci, although FANCD2 monoubiquitination in the FA pathway was unaffected. RUNX1 and RUNX3 interact with FANCD2 independently of CBFβ, suggesting a nontranscriptional role for RUNX in DNA repair. These findings suggest that RUNX dysfunction causes DNA repair defect, besides transcriptional misregulation, and promotes the development of leukemias and other cancers.
journal_name
Cell Repjournal_title
Cell reportsauthors
Wang CQ,Krishnan V,Tay LS,Chin DW,Koh CP,Chooi JY,Nah GS,Du L,Jacob B,Yamashita N,Lai SK,Tan TZ,Mori S,Tanuichi I,Tergaonkar V,Ito Y,Osato Mdoi
10.1016/j.celrep.2014.06.046subject
Has Abstractpub_date
2014-08-07 00:00:00pages
767-82issue
3issn
2211-1247pii
S2211-1247(14)00530-0journal_volume
8pub_type
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