Abstract：
:The rate of protein synthesis varies according to the mRNA sequence in ways that affect gene expression. Global analysis of translational pausing is now possible with ribosome profiling. Here, we revisit an earlier report that Shine-Dalgarno sequences are the major determinant of translational pausing in bacteria. Using refinements in the profiling method as well as biochemical assays, we find that SD motifs have little (if any) effect on elongation rates. We argue that earlier evidence of pausing arose from two factors. First, in previous analyses, pauses at Gly codons were difficult to distinguish from pauses at SD motifs. Second, and more importantly, the initial study preferentially isolated long ribosome-protected mRNA fragments that are enriched in SD motifs. These findings clarify the landscape of translational pausing in bacteria as observed by ribosome profiling.
journal_name
Cell Repjournal_title
Cell reportsauthors
Mohammad F,Woolstenhulme CJ,Green R,Buskirk ARdoi
10.1016/j.celrep.2015.12.073subject
Has Abstractpub_date
2016-02-02 00:00:00pages
686-694issue
4issn
2211-1247pii
S2211-1247(15)01529-6journal_volume
14pub_type
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