Abstract：
:The 26S proteasome is the central ATP-dependent protease in eukaryotes and is essential for organismal health. Proteasome assembly is mediated by several dedicated, evolutionarily conserved chaperone proteins. These chaperones associate transiently with assembly intermediates but are absent from mature proteasomes. Chaperone eviction upon completion of proteasome assembly is necessary for normal proteasome function, but how they are released remains unresolved. Here, we demonstrate that the Nas6 assembly chaperone, homolog of the human oncogene gankyrin, is evicted from nascent proteasomes during completion of assembly via a conformation-specific allosteric interaction of the Rpn5 subunit with the proteasomal ATPase ring. Subsequent ATP binding by the ATPase subunit Rpt3 promotes conformational remodeling of the ATPase ring that evicts Nas6 from the nascent proteasome. Our study demonstrates how assembly-coupled allosteric signals promote chaperone eviction and provides a framework for understanding the eviction of other chaperones from this biomedically important molecular machine.
journal_name
Cell Repjournal_title
Cell reportsauthors
Nemec AA,Peterson AK,Warnock JL,Reed RG,Tomko RJ Jrdoi
10.1016/j.celrep.2018.12.042subject
Has Abstractpub_date
2019-01-08 00:00:00pages
483-495.e5issue
2issn
2211-1247pii
S2211-1247(18)31972-7journal_volume
26pub_type
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