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Synthesis of Isomeric Phosphoubiquitin Chains Reveals that Phosphorylation Controls Deubiquitinase Activity and Specificity.

Abstract：

:Ubiquitin is post-translationally modified by phosphorylation at several sites, but the consequences of these modifications are largely unknown. Here, we synthesize multi-milligram quantities of ubiquitin phosphorylated at serine 20, serine 57, and serine 65 via genetic code expansion. We use these phosphoubiquitins for the enzymatic assembly of 20 isomeric phosphoubiquitin dimers, with different sites of isopeptide linkage and/or phosphorylation. We discover that phosphorylation of serine 20 on ubiquitin converts UBE3C from a dual-specificity E3 ligase into a ligase that primarily synthesizes K48 chains. We profile the activity of 31 deubiquitinases on the isomeric phosphoubiquitin dimers in 837 reactions, and we discover that phosphorylation at distinct sites in ubiquitin can activate or repress cleavage of a particular linkage by deubiquitinases and that phosphorylation at a single site in ubiquitin can control the specificity of deubiquitinases for distinct ubiquitin linkages.

journal_name

Cell Rep

journal_title

Cell reports

authors

Huguenin-Dezot N,De Cesare V,Peltier J,Knebel A,Kristaryianto YA,Rogerson DT,Kulathu Y,Trost M,Chin JW

doi

10.1016/j.celrep.2016.06.064

subject

Has Abstract

pub_date

2016-07-26 00:00:00

pages

1180-1193

issue

4

issn

2211-1247

pii

S2211-1247(16)30818-X

journal_volume

16

pub_type

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