Abstract:
:As three-dimensional cell culture formats gain in popularity, there emerges a need for tools that produce vast amounts of data on individual cells within the spheroids or organoids. Here, we present a microfluidic platform that provides access to such data by parallelizing the manipulation of individual spheroids within anchored droplets. Different conditions can be applied in a single device by triggering the merging of new droplets with the spheroid-containing drops. This allows cell-cell interactions to be initiated for building microtissues, studying stem cells' self-organization, or observing antagonistic interactions. It also allows the spheroids' physical or chemical environment to be modulated, as we show by applying a drug over a large range of concentrations in a single parallelized experiment. This convergence of microfluidics and image acquisition leads to a data-driven approach that allows the heterogeneity of 3D culture behavior to be addressed across the scales, bridging single-cell measurements with population measurements.
journal_name
Cell Repjournal_title
Cell reportsauthors
Tomasi RF,Sart S,Champetier T,Baroud CNdoi
10.1016/j.celrep.2020.107670subject
Has Abstractpub_date
2020-05-26 00:00:00pages
107670issue
8issn
2211-1247pii
S2211-1247(20)30623-9journal_volume
31pub_type
杂志文章相关文献
Cell Reports文献大全abstract::Two-photon imaging with genetically encoded calcium indicators (GECIs) enables long-term observation of neuronal activity in vivo. However, there are very few studies of GECIs in primates. Here, we report a method for long-term imaging of a GECI, GCaMP6f, expressed from adeno-associated virus vectors in cortical neuro...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.10.050
更新日期:2015-12-01 00:00:00
abstract::Tissue-resident memory T (TRM) cells provide key adaptive immune responses in infection, cancer, and autoimmunity. However, transcriptional heterogeneity of human intestinal TRM cells remains undefined. Here, we investigate transcriptional and functional heterogeneity of human TRM cells through study of donor-derived ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108661
更新日期:2021-01-19 00:00:00
abstract::The joint contribution of pre-existing and de novo genetic variation to clonal adaptation is poorly understood but essential to designing successful antimicrobial or cancer therapies. To address this, we evolve genetically diverse populations of budding yeast, S. cerevisiae, consisting of diploid cells with unique hap...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.09.046
更新日期:2017-10-17 00:00:00
abstract::Our knowledge of transcriptional heterogeneities in epithelial stem and progenitor cell compartments is limited. Epidermal basal cells sustain cutaneous tissue maintenance and drive wound healing. Previous studies have probed basal cell heterogeneity in stem and progenitor potential, but a comprehensive dissection of ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.02.091
更新日期:2020-03-17 00:00:00
abstract::Autism spectrum disorders (ASDs) are associated with mutations affecting synaptic components, including GluN2B-NMDA receptors (NMDARs) and neurobeachin (NBEA). NBEA participates in biosynthetic pathways to regulate synapse receptor targeting, synaptic function, cognition, and social behavior. However, the role of NBEA...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.04.112
更新日期:2018-05-29 00:00:00
abstract::Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. Although aging and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this, we used a redox proteomic technique, oxidative isotope-coded affinity tags (OxICAT)...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.05.033
更新日期:2015-06-30 00:00:00
abstract::Oligodendrocyte progenitors (OPs) arise from distinct ventral and dorsal domains within the ventricular germinal zones of the embryonic CNS. The functional significance, if any, of these different populations is not known. Using dual-color reporter mice to distinguish ventrally and dorsally derived OPs, we show that, ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.03.069
更新日期:2016-04-26 00:00:00
abstract::Inflammasome activation is associated with numerous diseases. However, in vivo detection of the activated inflammasome complex has been limited by a dearth of tools. We have developed transgenic mice that ectopically express the fluorescent adaptor protein, apoptosis-associated speck-like protein containing a caspase ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.06.011
更新日期:2016-07-12 00:00:00
abstract::CRISPR/Cas-based technologies have revolutionized genetic approaches to addressing a wide range of neurobiological questions. The ability of CRISPR/Cas to introduce mutations into target genes allows us to perform in vivo loss-of-function experiments without generating genetically engineered mice. However, the lack of...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.06.038
更新日期:2019-07-09 00:00:00
abstract::ASXL2 is an ETP family protein that interacts with PPARγ. We find that ASXL2-/- mice are insulin resistant, lipodystrophic, and fail to respond to a high-fat diet. Consistent with genetic variation at the ASXL2 locus and human bone mineral density, ASXL2-/- mice are also severely osteopetrotic because of failed osteoc...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.05.019
更新日期:2015-06-16 00:00:00
abstract::Graft-versus-host disease (GVHD) limits the success of allogeneic hematopoietic cell transplantation (allo-HCT). Lysosomal acid lipase (LAL) mediates the intrinsic lipolysis of cells to generate free fatty acids (FFAs), which play an essential role in the development, proliferation, and function of T cells. Here, we f...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108316
更新日期:2020-10-27 00:00:00
abstract::The number of essential telomerase components in the active ribonucleoprotein (RNP) has important implications for its mechanism of action yet is by and large unknown. We report that two differentially tagged TLC1 RNAs endogenously expressed in a heterozygous diploid and simultaneously detected via multi-color fluores...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.06.045
更新日期:2015-07-21 00:00:00
abstract::Mutations in CEP290, a transition zone protein in primary cilia, cause diverse ciliopathies, including Leber congenital amaurosis (LCA) and Joubert-syndrome and related disorders (JSRD). We examined cilia biogenesis and function in cells derived from CEP290-LCA and CEP290-JSRD patients. CEP290 protein was reduced in L...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.06.045
更新日期:2017-07-11 00:00:00
abstract::Piwi-interacting RNAs (piRNAs), a class of 26- to 32-nt non-coding RNAs (ncRNAs), function in germline development, transposon silencing, and epigenetic regulation. We performed deep sequencing and annotation of untreated and periodate-treated small RNA cDNA libraries from human fetal and adult germline and reference ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.09.030
更新日期:2015-10-27 00:00:00
abstract::Mosquito-transmitted malaria parasites infect hepatocytes and asymptomatically replicate as liver stages. Using RNA sequencing, we show that a rodent malaria liver-stage infection stimulates a robust innate immune response including type I interferon (IFN) and IFNγ pathways. Liver-stage infection is suppressed by thes...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.03.018
更新日期:2014-04-24 00:00:00
abstract::RNAi screens have implicated hundreds of host proteins as HIV-1 dependency factors (HDFs). While informative, these early studies overlap poorly due to false positives and false negatives. To ameliorate these issues, we combined information from the existing HDF screens together with new screens performed with multipl...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.09.031
更新日期:2014-10-23 00:00:00
abstract::To understand how the Bcl6 transcriptional repressor functions in the immune system, we disrupted its RD2 repression domain in mice. Bcl6RD2(MUT) mice exhibit a complete loss of germinal center (GC) formation but retain normal extrafollicular responses. Bcl6RD2(MUT) antigen-engaged B cells migrate to the interfollicul...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.07.059
更新日期:2014-09-11 00:00:00
abstract::Alternatively activated (M2) macrophages promote wound healing but weaken antimicrobial defenses. The mechanisms that enforce macrophage divergence and dictate the phenotypic and metabolic characteristics of M2 macrophages remain elusive. We show that alternative activation with interleukin (IL)-4 induces expression o...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.05.093
更新日期:2019-06-25 00:00:00
abstract::The mechanisms by which the major Polycomb group (PcG) complexes PRC1 and PRC2 are recruited to target sites in vertebrate cells are not well understood. Building on recent studies that determined a reciprocal relationship between DNA methylation and Polycomb activity, we demonstrate that, in methylation-deficient emb...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.04.012
更新日期:2014-06-12 00:00:00
abstract::The circadian clock generates biological rhythms of metabolic and physiological processes, including the sleep-wake cycle. We previously identified a missense mutation in the flavin adenine dinucleotide (FAD) binding pocket of CRYPTOCHROME2 (CRY2), a clock protein that causes human advanced sleep phase. This prompted ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.03.041
更新日期:2017-04-11 00:00:00
abstract::Adipocytes arise from the commitment and differentiation of adipose precursors in white adipose tissue (WAT). In studying adipogenesis, precursor markers, including Pref-1 and PDGFRα, are used to isolate precursors from stromal vascular fractions of WAT, but the relation among the markers is not known. Here, we used t...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.09.086
更新日期:2018-10-23 00:00:00
abstract::Thymic epithelial cells (TECs) are critically required for T cell development, but the cellular mechanisms that maintain adult TECs are poorly understood. Here, we show that a previously unidentified subpopulation, EpCam(+)UEA1(-)Ly-51(+)PLET1(+)MHC class II(hi), which comprises <0.5% of adult TECs, contains bipotent ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.02.080
更新日期:2016-03-29 00:00:00
abstract::Isotope-based assessment of metabolic flux is achieved through a judicious balance of measurements and assumptions. Recent publications debate the validity of key assumptions used to model stable isotope labeling of liver metabolism in vivo. Here, we examine the controversy surrounding estimates of liver citric acid c...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.107986
更新日期:2020-08-04 00:00:00
abstract::Oxytocin and its receptor (Oxtr) play a crucial role in the postnatal transition of neuronal GABA neurotransmission from excitatory to inhibitory, a developmental process known as the GABA switch. Using hippocampal neurons from Oxtr-null mice, we show that (1) Oxtr is necessary for the correct timing of the GABA switc...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.03.013
更新日期:2016-04-05 00:00:00
abstract::MicroRNAs (miRNAs) are typically generated as ~22-nucleotide double-stranded RNAs via the processing of precursor hairpins by the ribonuclease III enzyme Dicer, after which they are loaded into Argonaute (Ago) proteins to form an RNA-induced silencing complex (RISC). However, the biogenesis of miR-451, an erythropoiet...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.09.029
更新日期:2013-11-14 00:00:00
abstract::In Drosophila larvae, the class IV dendritic arborization (da) neurons are polymodal nociceptors. Here, we show that ppk26 (CG8546) plays an important role in mechanical nociception in class IV da neurons. Our immunohistochemical and functional results demonstrate that ppk26 is specifically expressed in class IV da ne...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.10.020
更新日期:2014-11-20 00:00:00
abstract::Genetic and clinical association studies have identified disrupted in schizophrenia 1 (DISC1) as a candidate risk gene for major mental illness. DISC1 is interrupted by a balanced chr(1;11) translocation in a Scottish family in which the translocation predisposes to psychiatric disorders. We investigate the consequenc...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.07.061
更新日期:2015-09-01 00:00:00
abstract::Kappa opioid receptors (KORs) are involved in a variety of aversive behavioral states, including anxiety. To date, a circuit-based mechanism for KOR-driven anxiety has not been described. Here, we show that activation of KORs inhibits glutamate release from basolateral amygdala (BLA) inputs to the bed nucleus of the s...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.02.069
更新日期:2016-03-29 00:00:00
abstract::Hepatocyte nuclear factor 4α (HNF4α) is a dimeric transcription factor that controls as much as 60% of all liver genes. However, how it achieves such broad functional diversity is unknown. Here, we show that inflammation and immune pathway genes are differentially regulated in an isoform-dependent manner, confirming t...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.02.033
更新日期:2019-03-05 00:00:00
abstract::The serine/threonine protein phosphatase 5 (PP5) regulates multiple cellular signaling networks. A number of cellular factors, including heat shock protein 90 (Hsp90), promote the activation of PP5. However, it is unclear whether post-translational modifications also influence PP5 phosphatase activity. Here, we show a...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.10.074
更新日期:2017-11-14 00:00:00