Abstract：
:Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. Although aging and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this, we used a redox proteomic technique, oxidative isotope-coded affinity tags (OxICAT), to assess cysteine-residue redox changes in Drosophila melanogaster during aging and fasting. This approach enabled us to simultaneously identify and quantify the redox state of several hundred cysteine residues in vivo. Cysteine residues within young flies had a bimodal distribution with peaks at ∼10% and ∼85% reversibly oxidized. Surprisingly, these cysteine residues did not become more oxidized with age. In contrast, 24 hr of fasting dramatically oxidized cysteine residues that were reduced under fed conditions while also reducing cysteine residues that were initially oxidized. We conclude that fasting, but not aging, dramatically alters cysteine-residue redox status in D. melanogaster.
journal_name
Cell Repjournal_title
Cell reportsauthors
Menger KE,James AM,Cochemé HM,Harbour ME,Chouchani ET,Ding S,Fearnley IM,Partridge L,Murphy MPdoi
10.1016/j.celrep.2015.05.033subject
Has Abstractpub_date
2015-06-30 00:00:00pages
1856-65issue
12issn
2211-1247pii
S2211-1247(15)00569-0journal_volume
11pub_type
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