Abstract:
:In eukaryotic cells, inefficient splicing is surprisingly common and leads to the degradation of transcripts with retained introns. How pre-mRNAs are committed to nuclear decay is unknown. Here, we uncover a mechanism by which specific intron-containing transcripts are targeted for nuclear degradation in fission yeast. Sequence elements within these "decay-promoting" introns co-transcriptionally recruit the exosome specificity factor Mmi1, which induces degradation of the unspliced precursor and leads to a reduction in the levels of the spliced mRNA. This mechanism negatively regulates levels of the RNA helicase DDX5/Dbp2 to promote cell survival in response to stress. In contrast, fast removal of decay-promoting introns by co-transcriptional splicing precludes Mmi1 recruitment and relieves negative expression regulation. We propose that decay-promoting introns facilitate the regulation of gene expression. Based on the identification of multiple additional Mmi1 targets, including mRNAs, long non-coding RNAs, and sn/snoRNAs, we suggest a general role in RNA regulation for Mmi1 through transcript degradation.
journal_name
Cell Repjournal_title
Cell reportsauthors
Kilchert C,Wittmann S,Passoni M,Shah S,Granneman S,Vasiljeva Ldoi
10.1016/j.celrep.2015.11.026subject
Has Abstractpub_date
2015-12-22 00:00:00pages
2504-2515issue
11issn
2211-1247pii
S2211-1247(15)01326-1journal_volume
13pub_type
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