Abstract：
:This study evaluates HIV antibody responses and their evolution during the course of HIV infection. A phage display system is used to characterize antibody binding to >3,300 HIV peptides in 57 adults with early- to late-stage infection. We find that the number of unique epitopes targeted ("antibody breadth") increases early in infection and then stabilizes or declines. A decline in antibody breadth 9 months to 2 years after infection is associated with subsequent antiretroviral treatment (ART) initiation, and a faster decline in antibody breadth is associated with a shorter time to ART initiation. We identify 266 peptides with increasing antibody reactivity over time and 43 peptides with decreasing reactivity over time. These data are used to design a prototype four-peptide "serosignature" to predict duration of HIV infection. We also demonstrate that epitope engineering can be used to optimize peptide binding properties for applications such as cross-sectional HIV incidence estimation.
journal_name
Cell Repjournal_title
Cell reportsauthors
Eshleman SH,Laeyendecker O,Kammers K,Chen A,Sivay MV,Kottapalli S,Sie BM,Yuan T,Monaco DR,Mohan D,Wansley D,Kula T,Morrison C,Elledge SJ,Brookmeyer R,Ruczinski I,Larman HBdoi
10.1016/j.celrep.2019.03.097subject
Has Abstractpub_date
2019-04-30 00:00:00pages
1422-1433.e4issue
5issn
2211-1247pii
S2211-1247(19)30436-Xjournal_volume
27pub_type
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