Abstract:
:Sex determination is a unique process that allows the study of multipotent progenitors and their acquisition of sex-specific fates during differentiation of the gonad into a testis or an ovary. Using time series single-cell RNA sequencing (scRNA-seq) on ovarian Nr5a1-GFP+ somatic cells during sex determination, we identified a single population of early progenitors giving rise to both pre-granulosa cells and potential steroidogenic precursor cells. By comparing time series single-cell RNA sequencing of XX and XY somatic cells, we provide evidence that gonadal supporting cells are specified from these early progenitors by a non-sex-specific transcriptomic program before pre-granulosa and Sertoli cells acquire their sex-specific identity. In XX and XY steroidogenic precursors, similar transcriptomic profiles underlie the acquisition of cell fate but with XX cells exhibiting a relative delay. Our data provide an important resource, at single-cell resolution, for further interrogation of the molecular and cellular basis of mammalian sex determination.
journal_name
Cell Repjournal_title
Cell reportsauthors
Stévant I,Kühne F,Greenfield A,Chaboissier MC,Dermitzakis ET,Nef Sdoi
10.1016/j.celrep.2019.02.069subject
Has Abstractpub_date
2019-03-19 00:00:00pages
3272-3283.e3issue
12issn
2211-1247pii
S2211-1247(19)30248-7journal_volume
26pub_type
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