Dissecting Cell Lineage Specification and Sex Fate Determination in Gonadal Somatic Cells Using Single-Cell Transcriptomics.

Abstract:

:Sex determination is a unique process that allows the study of multipotent progenitors and their acquisition of sex-specific fates during differentiation of the gonad into a testis or an ovary. Using time series single-cell RNA sequencing (scRNA-seq) on ovarian Nr5a1-GFP+ somatic cells during sex determination, we identified a single population of early progenitors giving rise to both pre-granulosa cells and potential steroidogenic precursor cells. By comparing time series single-cell RNA sequencing of XX and XY somatic cells, we provide evidence that gonadal supporting cells are specified from these early progenitors by a non-sex-specific transcriptomic program before pre-granulosa and Sertoli cells acquire their sex-specific identity. In XX and XY steroidogenic precursors, similar transcriptomic profiles underlie the acquisition of cell fate but with XX cells exhibiting a relative delay. Our data provide an important resource, at single-cell resolution, for further interrogation of the molecular and cellular basis of mammalian sex determination.

journal_name

Cell Rep

journal_title

Cell reports

authors

Stévant I,Kühne F,Greenfield A,Chaboissier MC,Dermitzakis ET,Nef S

doi

10.1016/j.celrep.2019.02.069

subject

Has Abstract

pub_date

2019-03-19 00:00:00

pages

3272-3283.e3

issue

12

issn

2211-1247

pii

S2211-1247(19)30248-7

journal_volume

26

pub_type

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