Abstract：
:The enteroinvasive bacterium Shigella flexneri forces its uptake into non-phagocytic host cells through the translocation of T3SS effectors that subvert the actin cytoskeleton. Here, we report de novo actin polymerization after cellular entry around the bacterium-containing vacuole (BCV) leading to the formation of a dynamic actin cocoon. This cocoon is thicker than any described cellular actin structure and functions as a gatekeeper for the cytosolic access of the pathogen. Host CDC42, TOCA-1, N-WASP, WIP, the Arp2/3 complex, cortactin, coronin, and cofilin are recruited to the actin cocoon. They are subverted by T3SS effectors, such as IpgD, IpgB1, and IcsB. IcsB immobilizes components of the actin polymerization machinery at the BCV dependent on its fatty acyltransferase activity. This represents a unique microbial subversion strategy through localized entrapment of host actin regulators causing massive actin assembly. We propose that the cocoon promotes subsequent invasion steps for successful Shigella infection.
journal_name
Cell Repjournal_title
Cell reportsauthors
Kühn S,Bergqvist J,Gil M,Valenzuela C,Barrio L,Lebreton S,Zurzolo C,Enninga Jdoi
10.1016/j.celrep.2020.107638subject
Has Abstractpub_date
2020-05-12 00:00:00pages
107638issue
6issn
2211-1247pii
S2211-1247(20)30591-Xjournal_volume
31pub_type
杂志文章相关文献
Cell Reports文献大全abstract：:Kinase inhibitors represent the backbone of targeted cancer therapy, yet only a limited number of oncogenic drivers are directly druggable. By interrogating the activity of 1,505 kinase inhibitors, we found that BRD4-NUT-rearranged NUT midline carcinoma (NMC) cells are specifically killed by CDK9 inhibition (CDK9i) an...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.08.082
更新日期：2017-09-19 00:00:00
abstract：:During postnatal development, cerebellar climbing fibers alter their innervation strengths onto supernumerary Purkinje cell targets, generating a one-to-few connectivity pattern in adulthood. To get insight about the processes responsible for this remapping, we reconstructed serial electron microscopy datasets from mi...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.10.081
更新日期：2019-11-26 00:00:00
abstract：:Wnts are a family of 19 extracellular ligands that regulate cell fate, proliferation, and migration during metazoan embryogenesis and throughout adulthood. Wnts are acylated post-translationally at a conserved serine and bind the extracellular cysteine-rich domain (CRD) of Frizzled (FZD) seven-pass transmembrane recep...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.12.104
更新日期：2019-01-22 00:00:00
abstract：:The transcriptional response to infection with the bacterium Listeria monocytogenes (Lm) requires cooperative signals of the type I interferon (IFN-I)-stimulated JAK-STAT and proinflammatory NF-κB pathways. Using ChIP-seq analysis, we define genes induced in Lm-infected macrophages through synergistic transcriptional ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.06.021
更新日期：2015-07-14 00:00:00
abstract：:Genetic differences between humans and in vivo model systems, including mice and nonhuman primates, make it difficult to predict the efficacy of immunoglobulin G (IgG) activity in humans and understand the molecular and cellular mechanisms underlying that activity. To bridge this gap, we established a small-animal mod...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.02.041
更新日期：2014-04-10 00:00:00
abstract：:Glucagon, the principal hyperglycemic hormone, is secreted from pancreatic islet α cells as part of the counter-regulatory response to hypoglycemia. Hence, secretory output from α cells is under high demand in conditions of low glucose supply. Many tissues oxidize fat as an alternate energy substrate. Here, we show th...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.05.035
更新日期：2018-06-12 00:00:00
abstract：:In glioblastoma, invasion and proliferation are presumed to be mutually exclusive events; however, the molecular mechanisms that mediate this switch at the cellular level remain elusive. Previously, we have shown that phospho-OLIG2, a central-nervous-system-specific transcription factor, is essential for tumor growth ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.06.045
更新日期：2016-07-26 00:00:00
abstract：:The interferon (IFN)-induced transmembrane (IFITM) proteins are critical mediators of the host antiviral response. Here, we expand the role of IFITM proteins to host defense against intracellular bacterial infection by demonstrating that they restrict Mycobacterium tuberculosis (MTb) intracellular growth. Simultaneous...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.09.048
更新日期：2015-11-03 00:00:00
abstract：:Traditionally, hippocampal long-term potentiation (LTP) of synaptic strength requires Ca(2+)/calmodulin (CaM)-dependent protein kinase II (CaMKII) and other kinases, whereas long-term depression (LTD) requires phosphatases. Here, we found that LTD also requires CaMKII and its phospho-T286-induced "autonomous" (Ca(2+)-...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.01.005
更新日期：2014-02-13 00:00:00
abstract：:The Piwi-piRNA pathway is well known for its germline function, yet its somatic role remains elusive. We show here that Piwi is required autonomously not only for germline stem cell (GSC) but also for somatic cyst stem cell (CySC) maintenance in the Drosophila testis. Reducing Piwi activity in the testis caused defect...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.06.004
更新日期：2015-07-07 00:00:00
abstract：:Unconventional secretion of exosome vesicles from multivesicular endosomes (MVEs) occurs across a broad set of systems and is reported to be upregulated in cancer, where it promotes aggressive behavior. However, regulatory control of exosome secretion is poorly understood. Using cancer cells, we identified specialized...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2013.10.050
更新日期：2013-12-12 00:00:00
abstract：:Activation of CaV2.1 voltage-gated calcium channels is facilitated by preceding calcium entry. Such self-modulatory facilitation is thought to contribute to synaptic facilitation. Using knockin mice with mutated CaV2.1 channels that do not facilitate (Ca IM-AA mice), we surprisingly found that, under conditions of phy...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.01.114
更新日期：2019-02-26 00:00:00
abstract：:The distinct lineage potential is a key feature of hematopoietic stem cell (HSC) heterogeneity, but a subset of HSCs specialized for a single lymphoid compartment has not been identified. Here we report that HSCs expressing junctional adhesion molecule 2 (Jam2) at a higher level (Jam2high HSCs) have a greater T cell r...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.05.028
更新日期：2019-06-04 00:00:00
abstract：:Pneumonias caused by influenza A virus (IAV) co- and secondary bacterial infections are characterized by their severity and high mortality rate. Previously, we have shown that bacterial pore-forming toxin (PFT)-mediated necroptosis is a key driver of acute lung injury during bacterial pneumonia. Here, we evaluate the ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108062
更新日期：2020-08-25 00:00:00
abstract：:Parkinson's disease (PD) is a common neurodegenerative disorder that exhibits motor and non-motor symptoms, as well as pathological hallmarks, including dopaminergic (DA) neuron death and formation of α-synuclein (α-Syn) Lewy bodies. Cyclin-G-associated kinase (GAK), a PD susceptibility gene identified through genome-...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2017.01.005
更新日期：2017-01-31 00:00:00
abstract：:Senescence is a cellular response preventing tumorigenesis. The Ras oncogene is frequently activated or mutated in human cancers, but Ras activation is insufficient to transform primary cells. In a search for cooperating oncogenes, we identify the lysine demethylase JMJD2A/KDM4A. We show that JMJD2A functions as a neg...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2012.09.033
更新日期：2012-11-29 00:00:00
abstract：:Fragile X syndrome (FXS) is the leading heritable cause of intellectual disability and commonly co-occurs with autism spectrum disorder. Silencing of the Fmr1 gene leads to the absence of the protein product, fragile X mental retardation protein (FMRP), which represses translation of many target mRNAs. Excess translat...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.08.001
更新日期：2019-09-03 00:00:00
abstract：:Indoleamine 2,3-dioxygenase (IDO) has been described as a major mechanism of immunosuppression in tumors, though the mechanisms of this are poorly understood. Here, we find that expression of IDO by tumor cells results in aggressive tumor growth and resistance to T-cell-targeting immunotherapies. We demonstrate that I...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.08.077
更新日期：2015-10-13 00:00:00
abstract：:Durable humoral immunity against epidemic infectious disease requires the survival of long-lived plasma cells (LLPCs). LLPC longevity is dependent on metabolic programs distinct from short-lived plasma cells (SLPCs); however, the mechanistic basis for this difference is unclear. We have previously shown that CD28, the...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.107815
更新日期：2020-06-23 00:00:00
abstract：:The vascular endothelial growth factor-A (VEGF-A)-VEGFR2 pathway drives tumor vascularization by activating proangiogenic signaling in endothelial cells (ECs). Here, we show that EC-sphingosine-1-phosphate receptor 1 (S1PR1) amplifies VEGFR2-mediated angiogenic signaling to enhance tumor growth. We show that cancer ce...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2019.11.036
更新日期：2019-12-10 00:00:00
abstract：:Fear extinction is a component of cognitive flexibility that is relevant for important psychiatric diseases, but its molecular mechanism is still largely elusive. We established mice lacking the kinesin-4 motor KIF21B as a model for fear extinction defects. Postsynaptic NMDAR-dependent long-term depression (LTD) is sp...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.05.089
更新日期：2018-06-26 00:00:00
abstract：:Understanding how antibiotics impact bacterial metabolism may provide insight into their mechanisms of action and could lead to enhanced therapeutic methodologies. Here, we profiled the metabolome of Escherichia coli after treatment with three different classes of bactericidal antibiotics (?-lactams, aminoglycosides, ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.09.059
更新日期：2015-11-03 00:00:00
abstract：:During chronic infection, the inflammatory cytokine interferon gamma (IFNγ) damages hematopoietic stem cells (HSCs) by disrupting quiescence and promoting excessive terminal differentiation. However, the mechanism by which IFNγ hinders HSC quiescence remains undefined. Using intravital 3-dimensional microscopy, we fin...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2020.108530
更新日期：2020-12-22 00:00:00
abstract：:Modeling late-onset disorders such as Parkinson's disease (PD) using iPSC technology remains a challenge, as current differentiation protocols yield cells with the properties of fetal-stage cells. Here, we tested whether it is possible to accelerate aging in vitro to trigger late-onset disease phenotypes in an iPSC mo...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.09.062
更新日期：2016-10-18 00:00:00
abstract：:Mcm2-7 helicase is loaded onto double-stranded origin DNA as an inactive double hexamer (DH) in G1 phase. The mechanisms of Mcm2-7 remodeling that trigger helicase activation in S phase remain unknown. Here, we develop an approach to detect and purify the endogenous DHs directly. Through cellular fractionation, we pro...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.11.018
更新日期：2015-12-22 00:00:00
abstract：:The Fanconi anemia (FA) pathway is critically involved in the maintenance of hematopoietic stem cells and the suppression of carcinogenesis. A key FA protein, FANCD2, is monoubiquitinated and accumulates in chromatin in response to DNA interstrand crosslinks (ICLs), where it coordinates DNA repair through mechanisms t...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2014.04.005
更新日期：2014-05-22 00:00:00
abstract：:Endosomal sorting complexes required for transport (ESCRT) drive cell surface receptor degradation resulting in attenuation of oncogenic signaling and pointing to a tumor suppressor function. Here, we show that loss of function of an ESCRT protein (HD-PTP encoded by the PTPN23 gene, located on the tumor suppressor gen...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.04.076
更新日期：2016-05-31 00:00:00
abstract：:Exposure to low-dose irradiation causes transiently elevated expression of the long ncRNA PARTICLE (gene PARTICLE, promoter of MAT2A-antisense radiation-induced circulating lncRNA). PARTICLE affords both a cytosolic scaffold for the tumor suppressor methionine adenosyltransferase (MAT2A) and a nuclear genetic platform...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2015.03.043
更新日期：2015-04-21 00:00:00
abstract：:Even though hyperthermia is a promising treatment for cancer, the relationship between specific temperatures and clinical benefits and predictors of sensitivity of cancer to hyperthermia is poorly understood. Ovarian and uterine tumors have diverse hyperthermia sensitivities. Integrative analyses of the specific gene ...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2016.10.020
更新日期：2016-11-01 00:00:00
abstract：:Alzheimer's disease (AD) is the most common form of dementia, impairing cognitive and motor functions. One of the pathological hallmarks of AD is neuronal loss, which is not reflected in mouse models of AD. Therefore, the role of neuronal death is still uncertain. Here, we used a Drosophila AD model expressing a secre...
journal_title：Cell reports
pub_type： 杂志文章
doi：10.1016/j.celrep.2018.11.098
更新日期：2018-12-26 00:00:00
