Abstract：
:The transmission of malaria parasites to mosquitoes relies on the rapid induction of sexual reproduction upon their ingestion into a blood meal. Haploid female and male gametocytes become activated and emerge from their host cells, and the males enter the cell cycle to produce eight microgametes. The synchronized nature of gametogenesis allowed us to investigate phosphorylation signaling during its first minute in Plasmodium berghei via a high-resolution time course of the phosphoproteome. This revealed an unexpectedly broad response, with proteins related to distinct cell cycle events undergoing simultaneous phosphoregulation. We implicate several protein kinases in the process, and we validate our analyses on the plant-like calcium-dependent protein kinase 4 (CDPK4) and a homolog of serine/arginine-rich protein kinases (SRPK1). Mutants in these kinases displayed distinct phosphoproteomic disruptions, consistent with differences in their phenotypes. The results reveal the central role of protein phosphorylation in the atypical cell cycle regulation of a divergent eukaryote.
journal_name
Cell Repjournal_title
Cell reportsauthors
Invergo BM,Brochet M,Yu L,Choudhary J,Beltrao P,Billker Odoi
10.1016/j.celrep.2017.10.071subject
Has Abstractpub_date
2017-11-14 00:00:00pages
2017-2029issue
7issn
2211-1247pii
S2211-1247(17)31534-6journal_volume
21pub_type
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