Abstract：
:Although bacterial cell morphology is tightly controlled, the principles of size regulation remain elusive. In Escherichia coli, perturbation of cell-wall synthesis often results in similar morphologies, making it difficult to deconvolve the complex genotype-phenotype relationships underlying morphogenesis. Here we modulated cell width through heterologous expression of sequences encoding the essential enzyme PBP2 and through sublethal treatments with drugs that inhibit PBP2 and the MreB cytoskeleton. We quantified the biochemical and biophysical properties of the cell wall across a wide range of cell sizes. We find that, although cell-wall chemical composition is unaltered, MreB dynamics, cell twisting, and cellular mechanics exhibit systematic large-scale changes consistent with altered chirality and a more isotropic cell wall. This multiscale analysis enabled identification of distinct roles for MreB and PBP2, despite having similar morphological effects when depleted. Altogether, our results highlight the robustness of cell-wall synthesis and physical principles dictating cell-size control.
journal_name
Cell Repjournal_title
Cell reportsauthors
Tropini C,Lee TK,Hsin J,Desmarais SM,Ursell T,Monds RD,Huang KCdoi
10.1016/j.celrep.2014.10.027subject
Has Abstractpub_date
2014-11-20 00:00:00pages
1520-7issue
4issn
2211-1247pii
S2211-1247(14)00881-Xjournal_volume
9pub_type
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