Abstract:
:Permethrin is a synthetic pyrethroid extensively used as anti-woodworm agent and for indoor and outdoor pest control. The main route of human exposure is through fruit, vegetable and milk intake. Low dosage exposure to permethrin during neonatal brain development (from postnatal day 6 to postnatal day 21) leads to dopamine decrease in rat striatum nucleus, oxidative stress and behavioural changes linked to the development of Parkinson's like neurodegeneration later in life. The aim of this study was to evaluate the expression of genes involved in the dopaminergic pathway and epigenetic regulatory mechanisms in adolescent rats treated with permethrin during neonatal brain development. Furthermore, in order to shed light on the mechanisms associated with molecular impairments, in silico studies were performed. The outcomes show increased expression of genes related to the dopamine-synthesis pathway (Nurr1, Th, Snca), epigenetics (TET proteins and Mecp2) and exposure to toxicants (Pon1 and Pon2) in adolescent rats compared with control group. Furthermore, increased global 5mC and 5hmC levels were observed in the DNA extracted from striatum of early-life treated rats in comparison with controls. FAIRE-qPCR analysis shows that permethrin induces an enrichment of chromatin-free DNA at the level of Th and Nurr1 promoters, and ChIP-qPCR reveals a significant reduction in methylation levels at H3K9me3 position at both Th and Nurr1 promoter regions. In silico studies show that permethrin competes for the same two binding sites of known NURR1 agonists, with a lower binding free energy for permethrin, suggesting an important durable association of permethrin with the orphan receptor. Moreover, alpha-synuclein shows a strong affinity for NURR1, corroborating previous experimental outcomes on the interactions between them. This study focuses on an emerging role of early-life exposure to environmental pollutants in the regulation of late onset diseases through intriguing mechanisms that change crucial epigenetic patterns starting from adolescent age.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Bordoni L,Nasuti C,Fedeli D,Galeazzi R,Laudadio E,Massaccesi L,López-Rodas G,Gabbianelli Rdoi
10.1016/j.exger.2019.06.002subject
Has Abstractpub_date
2019-09-01 00:00:00pages
110629eissn
0531-5565issn
1873-6815pii
S0531-5565(19)30052-Xjournal_volume
124pub_type
杂志文章abstract::Cinnamon is a spice commonly used worldwide to flavor desserts, fruits, cereals, breads, and meats. Numerous health benefits have been attributed to its consumption, including the recent suggestion that it may decrease blood glucose levels in people with diabetes. Insulin signaling is an integral pathway regulating th...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2014.09.019
更新日期:2014-12-01 00:00:00
abstract:OBJECTIVES:To date studies evaluating the relation between circulating aldosterone levels and mortality in elderly female individuals are lacking. We therefore aimed to assess the relationship between circulating aldosterone levels and mortality in a population-based cohort study of female nursing home residents. METH...
journal_title:Experimental gerontology
pub_type: 杂志文章,多中心研究
doi:10.1016/j.exger.2013.01.004
更新日期:2013-03-01 00:00:00
abstract::Telomeres serve as a mitotic clock and biological marker of senescence. Diabetes mellitus (DM) is associated with damage to target organs and premature aging. We assessed the effect of glycemic control on telomere dynamics in arterial cells of 58 patients undergoing coronary artery bypass and in mononuclear blood cell...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2007.07.005
更新日期:2007-10-01 00:00:00
abstract::Aging is associated with altered immune responses, particularly with a diminished CD8 T cell response. Although both intrinsic and extrinsic factors are hypothesized to impact this decreased T cell response, the direct evidence of an intrinsic deficiency in virus-specific CD8 T cells is limited. In this study, a TCR t...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2013.02.027
更新日期:2013-06-01 00:00:00
abstract::Threat-related changes in postural control and their associations with changes in emotional and cognitive states are influenced by postural threat experience, however, limited work has explored individuals' capacity to adapt threat-related responses over longer periods of threat exposure. This study examined the effec...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2019.04.015
更新日期:2019-07-15 00:00:00
abstract::Caloric restriction (CR) without malnutrition in mice and rats reduces the incidence of spontaneous tumors and delays their appearance while increasing maximum life span. These results depend largely on CR per se, and not on low intakes of fat or other nutrients. Although most studies have tested CR imposed early in l...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/0531-5565(92)90012-o
更新日期:1992-09-01 00:00:00
abstract::As the analysis of apoptosis is of interest in many basic and clinically oriented investigations, we intend to give a brief overview on the recently most-used methods for detection of apoptotic cells, including the study of morphology, analysis of DNA degradation, DNA end-labeling techniques, flow cytometric analysis,...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/s0531-5565(98)00031-x
更新日期:1998-09-01 00:00:00
abstract::Plasma and pituitary concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and prolactin (Prl) were determined by radioimmunoassay in young (2-4 months-old) and aged CD-1 (14-18 months-old) and C57BL/6 (16-22 months-old) mice one month after ovariectomy. In young, ovariectomized mice, plasma ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/s0531-5565(82)80005-3
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND:Awareness of physical ability may impact fall risk during everyday tasks. Therefore, we investigated perceived reach (PR; estimation of furthest reach distance), maximal reach (MR) and reach judgement error (RJE), and their relationships with neuropsychological and physical performance, and falls in older pe...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2018.10.020
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVE:To compare body composition as assessed by conventional and vector bioelectrical impedance analysis according to the nutritional cataloging using body mass index (BMI) in a group of institutionalized elderly. METHODS:Cross-sectional study in 38 institutionalized elderly. Body composition was estimated by bio...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2016.10.002
更新日期:2016-12-01 00:00:00
abstract::Alpha-synuclein (α-Syn) accumulation is significantly correlated with motor deficits and mitochondrial dysfunction in Parkinson's disease (PD), but the molecular mechanism underlying its pathogenesis is unclear. In this study, we investigated the effects of treadmill exercise on motor deficits and mitochondrial dysfun...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2017.01.001
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVES:The present study investigated whether the performance on executive function tasks of patients with type 2 diabetes mellitus (T2DM) is as impaired as that of Alzheimer's disease (AD) patients and to compare their performance with that of a group of cognitively healthy older adults. We also investigated wheth...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2016.07.013
更新日期:2016-10-01 00:00:00
abstract::Degeneration of tongue muscles with aging may contribute to swallowing deficits observed in elderly people. However, the capacity for tongue muscle stem cells (SCs) to regenerate and repair the aged tongue and improve tongue strength following tongue exercise (a current clinical treatment) has never been examined. We ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2020.111104
更新日期:2020-12-01 00:00:00
abstract::Culturing Caenorhabditis elegans in axenic medium leads to a twofold increase in lifespan and considering the similar phenotypical traits with dietary restricted animals, it is referred to as axenic dietary restriction (ADR). The free radical theory of aging has suggested a pivotal role for mitochondria in the aging p...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2014.02.009
更新日期:2014-08-01 00:00:00
abstract::In the present paper, we investigated whether the phenotype, the antigen-presenting capacity, and the migration of antigen-presenting cells (APCs), are affected by the aging process. APCs were obtained incubating peritoneal monocyte-macrophage cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) (immat...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/s0531-5565(02)00087-6
更新日期:2002-08-01 00:00:00
abstract::Fatigue is a common complaint in older people. Laboratory-induced muscle fatigue has been found to affect physical functions in older populations but these protocols are rigorous and are unlikely to accurately reflect daily activities. This study used an ecological approach to determine the effects of a busy day on se...
journal_title:Experimental gerontology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.exger.2018.03.009
更新日期:2018-06-01 00:00:00
abstract::Aging adipose tissues (ATs) manifest reduced vascularity and increased hypoxia and inflammation that contribute to local and systemic metabolic dysfunction. However, the mechanisms that underlie these age-related changes are incompletely understood. In this study, we sought to examine insulin-stimulated vasodilation a...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2020.111126
更新日期:2020-12-01 00:00:00
abstract::Neural correlates of placebo analgesia (PA) in patients with neurocognitive disorders have not yet been elucidated. The present study aimed to evaluate how and to what extent executive (dys)functions of the medial prefrontal cortex (MPFC) may be related to PA. To this end, twenty-three subjects complaining of differen...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2019.01.011
更新日期:2019-04-01 00:00:00
abstract::Dystrophin-deficiency causes cardiomyopathies and shortens the life expectancy of Duchenne and Becker muscular dystrophy patients. Restoring Dystrophin expression in the heart by gene transfer is a promising avenue to explore as a therapy. Truncated Dystrophin gene constructs have been engineered and shown to alleviat...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2013.10.015
更新日期:2014-01-01 00:00:00
abstract:AIM:Sirtuins are proteins that connect energy metabolism, oxidative stress and aging. Expression of heat shock proteins (Hsps) is regulated by heat shock factors (HSFs) in response to various environmental and physiological stresses, such as oxidative stress. Oxidative stress accumulates during aging which makes cells ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2016.03.015
更新日期:2016-06-15 00:00:00
abstract::This study investigated the effects of two training programmes of 6 weeks combining strength and balance exercises in different proportions. One training programme [n=10; 71.4 (6.3) years] consisted mainly of strength exercises (ST) and the other programme [n=8; 71.4 (6.4) years] included a majority of balance exercis...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2014.11.013
更新日期:2015-01-01 00:00:00
abstract::Sex hormones are known to help maintaining the cognitive ability in male and female rats. Hypogonadism results in the reduction of the dendritic spines of central neurons which is believed to undermine memory and cognition and cause fatigue and poor concentration. In our previous studies, we have reported age-related ...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2014.06.010
更新日期:2014-09-01 00:00:00
abstract::Aging has a profound effect on the immune system, and both innate and adaptive arms of the immune system show functional decline with age. In response to infection with intracellular microorganisms, old animals mobilize decreased numbers of antigen-specific CD8+ T cells with reduced production of effector molecules an...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2018.01.011
更新日期:2018-05-01 00:00:00
abstract::Aging is the primary risk factor for functional decline; thus, understanding and preventing disability among older adults has emerged as an important public health challenge of the 21st century. The science of gerontology - or geroscience - has the practical purpose of "adding life to the years." The overall goal of g...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2020.111123
更新日期:2020-12-01 00:00:00
abstract::We tested the hypothesis that hesperidin would reverse age-related aortic stiffness, perivascular adipose (PVAT) mediated-arterial stiffening and PVAT advanced glycation end-products (AGE) accumulation. Aortic pulse wave velocity (aPWV) and intrinsic mechanical stiffness, two measures of arterial stiffness, were asses...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2017.08.003
更新日期:2017-10-15 00:00:00
abstract::Stem cells are located throughout the adult body of higher organisms, supporting a continuous renewal and repair of tissues. Unique abilities of stem cells are self-renewal and multipotential differentiation. It is, therefore, of critical importance for an organism to maintain and control quantity and quality of stem ...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2005.07.006
更新日期:2005-12-01 00:00:00
abstract::Psychological stress has often been described as a feeling of being overwhelmed by the necessity of constant adjustment to an individual's changing environment. Stress affects people of all ages, but the lives of the elderly may particularly be affected. Major changes can cause anxiety leading to feelings of insecurit...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2014.12.003
更新日期:2015-08-01 00:00:00
abstract::Changes associated with the age-related decline of physiological functions, and their relation with mortality rates, are thoroughly being investigated in the aging research field. We used the Mediterranean fruit fly Ceratitis capitata, largely studied by biodemographers, as a model for functional senescence studies. T...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2012.04.001
更新日期:2012-06-01 00:00:00
abstract::Advanced paternal age is associated with increased complications in pregnancy and genetic diseases in offspring. Oxidative stress is a major contributor to the damage accumulated in sperm during aging. Complex networks of antioxidants regulate reactive oxygen species (ROS) in the testis. While mounting evident shows t...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2016.08.012
更新日期:2016-11-01 00:00:00
abstract::Several studies have shown that centenarians have better cardiovascular risk profiles compared to younger old people. Some reports have revealed that cardiovascular diseases (i.e. hypertension, diabetes, angina and/or myocardial infarction) are less common in centenarians respect to 70 and 80 years old persons. In ord...
journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2007.06.009
更新日期:2008-02-01 00:00:00