Mitochondrial Membrane Potential Regulates Nuclear Gene Expression in Macrophages Exposed to Prostaglandin E2.


:Metabolic engagement is intrinsic to immune cell function. Prostaglandin E2 (PGE2) has been shown to modulate macrophage activation, yet how PGE2 might affect metabolism is unclear. Here, we show that PGE2 caused mitochondrial membrane potential (Δψm) to dissipate in interleukin-4-activated (M(IL-4)) macrophages. Effects on Δψm were a consequence of PGE2-initiated transcriptional regulation of genes, particularly Got1, in the malate-aspartate shuttle (MAS). Reduced Δψm caused alterations in the expression of 126 voltage-regulated genes (VRGs), including those encoding resistin-like molecule α (RELMα), a key marker of M(IL-4) cells, and genes that regulate the cell cycle. The transcription factor ETS variant 1 (ETV1) played a role in the regulation of 38% of the VRGs. These results reveal ETV1 as a Δψm-sensitive transcription factor and Δψm as a mediator of mitochondrial-directed nuclear gene expression.






Sanin DE,Matsushita M,Klein Geltink RI,Grzes KM,van Teijlingen Bakker N,Corrado M,Kabat AM,Buck MD,Qiu J,Lawless SJ,Cameron AM,Villa M,Baixauli F,Patterson AE,Hässler F,Curtis JD,O'Neill CM,O'Sullivan D,Wu D,Mittler




Has Abstract


2018-12-18 00:00:00
















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    authors: Pimentel-Muiños FX,Seed B

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    authors: Stetson DB,Mohrs M,Mallet-Designe V,Teyton L,Locksley RM

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    authors: Afonina IS,Müller C,Martin SJ,Beyaert R

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  • Inhaled Fine Particles Induce Alveolar Macrophage Death and Interleukin-1α Release to Promote Inducible Bronchus-Associated Lymphoid Tissue Formation.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Siggs OM,Miosge LA,Yates AL,Kucharska EM,Sheahan D,Brdicka T,Weiss A,Liston A,Goodnow CC

    更新日期:2007-12-01 00:00:00

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    pub_type: 杂志文章,评审


    authors: Lambrecht BN,Hammad H,Fahy JV

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    pub_type: 杂志文章


    authors: Ahern PP,Schiering C,Buonocore S,McGeachy MJ,Cua DJ,Maloy KJ,Powrie F

    更新日期:2010-08-27 00:00:00