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The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development.

Abstract：

:Previous in vitro studies defined the minimal regions of RAG1 and RAG2 essential for V(D)J recombination. In order to characterize the role of the C-terminal "dispensable" portion of RAG2, we generated core-RAG2 knock-in mice. We found that the core-RAG2-containing recombinase complex is selectively defective in catalyzing V-to-DJ rearrangement at the IgH and TCRbeta loci, resulting in partial developmental blocks in B and T lymphopoiesis. Analysis of recombination intermediates showed defects at the cleavage phase of the reaction. We also observed a reduction in overall recombinase activity in core-RAG2-expressing thymocytes, leading us to suggest that the interaction of a defective recombinase with RSS sequences unique to VH and Vbeta gene segments may underlie the specific V-to-DJ rearrangement defect in core-RAG2 mice.

journal_name

Immunity

journal_title

Immunity

authors

Liang HE,Hsu LY,Cado D,Cowell LG,Kelsoe G,Schlissel MS

doi

10.1016/s1074-7613(02)00448-x

keywords:

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

639-51

issue

5

eissn

1074-7613

issn

1097-4180

pii

S1074-7613(02)00448-X

journal_volume

17

pub_type

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