当前位置： SCI文献检索 > Stem Cell Reports期刊下所有文献 > The MicroRNA-371 Family as Plasma Biomarkers for Monitoring Undifferentiated and Potentially Malignant Human Pluripotent Stem Cells in Teratoma Assays.

The MicroRNA-371 Family as Plasma Biomarkers for Monitoring Undifferentiated and Potentially Malignant Human Pluripotent Stem Cells in Teratoma Assays.

Abstract：

:Predicting developmental potency and risk of posttransplantation tumor formation by human pluripotent stem cells (hPSCs) and their derivatives largely rely on classical histological analysis of teratomas. Here, we investigated whether an assay based on microRNAs (miRNA) in blood plasma is able to detect potentially malignant elements. Several hPSCs and human malignant germ cell tumor (hGCT) lines were investigated in vitro and in vivo after mouse xenografting. The multiple conventional hPSC lines generated mature teratomas, while xenografts from induced hPSCs (hiPSCs) with reactivated reprogramming transgenes and hGCT lines contained undifferentiated and potentially malignant components. The presence of these elements was reflected in the mRNA and miRNA profiles of the xenografts with OCT3/4 mRNA and the miR-371 and miR-302 families readily detectable. miR-371 family members were also identified in mouse plasma faithfully reporting undifferentiated elements in the xenografts. This study demonstrated that undifferentiated and potentially malignant cells could be detected in vivo.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Salvatori DCF,Dorssers LCJ,Gillis AJM,Perretta G,van Agthoven T,Gomes Fernandes M,Stoop H,Prins JB,Oosterhuis JW,Mummery C,Looijenga LHJ

doi

10.1016/j.stemcr.2018.11.002

subject

Has Abstract

pub_date

2018-12-11 00:00:00

pages

1493-1505

issue

6

issn

2213-6711

pii

S2213-6711(18)30448-X

journal_volume

11

pub_type

杂志文章

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